• Title/Summary/Keyword: culture time

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Nonlinear Time-Varying Control Based on Differential Geometry

  • Lee, Jong-Yong;Jung, Kye-dong;Cho, Seongsoo;Strzelecki, Michat
    • International Journal of Internet, Broadcasting and Communication
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    • v.6 no.2
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    • pp.1-9
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    • 2014
  • This paper presents a study on nonlinear time varying systems based on differential geometry. A brief introduction about controllability and involutivity will be presented. As an example, the exact feedback linearization and the approximate feedback linearization are used in order to show some application examples.

Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad (소시지와 야채 샐러드에서 Yersinia enterocolitica 검출을 위한 배지법과 real-time PCR법의 비교)

  • Kim, Yun-Gyeong;Chon, Jung-Whan;Lee, Jae-Hoon;Kwak, Hyo-Sun;Hwang, In-Gyun;Seo, Kun-Ho
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.133-136
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    • 2013
  • The purpose of this study was to compare a conventional culture method and real-time PCR for the detection of Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y. enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasan-novobiocin agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-time PCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positive samples detected by culture method (49 out of 60) was similar (p>0.05) with that of real-time PCR (50 out of 60). However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p<0.05) than that of the conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool for detecting Y. enterocolitica, particularly in food samples with high levels of background flora, such as a vegetable salad.

TaqMan Probe Real-Time PCR for Quantitative Detection of Mycoplasma during Manufacture of Biologics (생물의약품 제조공정에서 마이코플라스마 정량 검출을 위한 TaqMan Probe Real-Time PCR)

  • Lee, Jae Il;Kim, In Seop
    • KSBB Journal
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    • v.29 no.5
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    • pp.361-371
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    • 2014
  • Mycoplasma is well recognized as one of the most prevalent and serious microbial contaminants of biologic manufacturing processes. Conventional methods for mycoplasma testing, direct culture method and indirect indicator cell culture method, are lengthy, costly and less sensitive to noncultivable species. In this report, we describe a new TaqMan probe-based real-time PCR method for rapid and quantitative detection of mycoplasma contamination during manufacture of biologics. Universal mycoplasma primers were used for mycoplasma PCR and mycoplasma DNA was quantified by use of a specific TaqMan probe. Specificity, sensitivity, and robustness of the real-time PCR method was validated according to the European Pharmacopoeia. The validation results met required criteria to justify its use as a replacement for the culture method. The established real-time PCR assay was successfully applied to the detection of mycoplasma from human keratinocyte and mesenchymal stem cell as well as Vero cell lines artificially infected with mycoplasma. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of mycoplasma contamination during manufacture of biologics.

Gender Differences of Leisure Time based on Simultaneous Activities (여가시간의 동시적 활동 분석을 통한 성차 연구)

  • Yoon, So-Young;Kim, Hyun
    • Journal of Family Resource Management and Policy Review
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    • v.11 no.2
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    • pp.85-93
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    • 2007
  • The purpose of this study was to assess gender differences in the quantity and quality of leisure time. It uses time diary for a nationally representative sample of Koreans(10 years old over) collected in 2004 by KNSO. This time use surveys provide information about a primary activity and a secondary activity (that is simultaneous activity accompanying the primary activity). The results was that married women experience less of leisure time and more contaminated leisure time by a secondary activity(especially household labor) than married men. This research presents that men and women have different quantity and quality of leisure time, and gender discrepancies persist in the experience of leisure time.

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Analysis of Trends in Housing Culture Until the Recent Year Since 1980s - Focused on the keywords of previous researches - (1980년대 이후 최근까지 주거문화에 관한 연구 경향 분석 - 선행연구의 주제어를 중심으로 -)

  • Kim, Young-Joo;Lee, Soo-Min;Kim, Eun-Bin;Park, Soo-Yeon
    • Korean Institute of Interior Design Journal
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    • v.22 no.4
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    • pp.104-112
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    • 2013
  • Housing culture can be defined as a subculture that characterizes a society, a region, and a time period. Therefore, identifying the housing needs of people is an effective way of understanding the housing culture. The purpose of this study was to identify the research trend of housing culture since 1980s until the recent year based on the keywords of previous literature. Seventy-one research articles were analyzed using content analysis as a research method and analysis framework which was composed of 4 aspects - physical, social, psychological, and living/welfare - was drawn for further analysis. As a main result, housing needs in social and living/welfare aspect were increasing as time passes. In general, researches related to physical aspect showed the highest ratio among 4 aspects. Specifically, research interests in community and social relationship among neighbors, natural environment, and housing welfare has remarkably increased since 2000s. The findings of this study showed that the housing culture is the comprehensive result or the process reflecting the needs and context in political, social, and economical background of those days.

Hydrogen Evolution by Mixed Culture of Clostridia with Rhodopseudornonas sphaeroides (Rhodopseudomonas sphaeroides와 Clostridia의 혼합)

  • Yi, Hye-Joo;Bae, Moo
    • Microbiology and Biotechnology Letters
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    • v.20 no.4
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    • pp.430-436
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    • 1992
  • Hydrogen evolution by mixed fermentation of Clostn"dium butyn"cum and photosynthetic bacteria which were capable of consuming clostridial metabolites and evolving hydrogen was investigated. Acetate and butyrate formed from anaerobic clostridial fermentation were efficiently utilized by Rhodopseudomonas sPhaeroides K-7. For complete bioconversion of clostridial metabolites such as acetate and butyrate into hydrogen, mixed culture of both anaerobic organisms forming molecular hydrogen was performed. By the mixed culture, the yield of hydrogen production increased by 20 to 75% and the levels of clostridial metabolites such as acetate, butyrate decreased in the fermentation broth. Influence of cell mixing ratio. mixing time and inoculum level on hydrogen evolution by mixed culture were examined. And then cometabolic pattern compared with in pure culture was observed as time course.

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System Thinking Perspective on the Dynamic Relationship between Organizational Characteristics of Nuclear Safety Culture

  • Kim, Byung Suk;Oh, Youngmin
    • Journal of the Ergonomics Society of Korea
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    • v.33 no.2
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    • pp.77-86
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    • 2014
  • Objective: The purpose of this study is to grasp the fundamental structure of incident occurrence in nuclear organizations based on system thinking, and analyze how various causes are interrelated in terms of the causal loop diagram. Background: The recent domestic and overseas nuclear power plant-related incidents and accidents are directly or indirectly associated with safety culture, and thus effective plans for the improvement of safety culture are being called for. While the safety of a nuclear power plant is highly dependent upon technology and equipment, the utilization, maintenance and inspection of the technology and equipment are conducted by workers of the nuclear power plant. Method: Methodology of system thinking perspective using causal loop analysis. Results: As a result of the analysis, first, it turned out that the fundamental cause of incident occurrence in nuclear organizations is time constraint. Second, if a workload of workers increases, their adherence to regulations and procedures comes to be reduced due to time constraint. Third, it is needed, through organizational learning education, to increase actions made from thoughts considering safety as the utmost priority in advance. Fourth, it is necessary to improve professionalism by enhancing educational programs for new workers, and to develop various scenarios with which they can cope with certain situations. Application: This paper provides a base for system dynamics simulation model for future study.

The Effects of Family-Friendly Culture on Employees' Work-Family Balance and Qualities of Lives (가족친화적 조직문화가 근로자의 일-가족 조화와 삶의 질에 미치는 영향)

  • Yoo, Gye-Sook
    • Journal of Families and Better Life
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    • v.26 no.5
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    • pp.27-37
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    • 2008
  • The main objective of this study was to examine the effects of family-friendly culture in various organizations in the private sectors on employee self-reported work-family balance and qualities of lives. It also attempted to clarify whether employee perceived work-family culture would vary according to size and type of organization, gender, and dual career partnership. The data came from 365 married full-time workers employed in a wide variety of organizations located in Seoul and GyeongGi-Do. The major findings of this study were as follows: Work-family culture was perceived more family-friendly in large-sized organizations and consumer industries. Female employees and workers in dual-career partnerships perceived the work-family culture of their organizations less family-friendly. The hierarchical multiple regression analyses revealed that firm size, supervisory support for employees' family activities, and the perception of negative career development opportunities as a consequence of utilizing family-friendly policies significantly predicted employees' work-family balance and qualities of lives, while organizational time demands having no significant effects on them. The implications of study results were discussed.

Food Consumption Discussion in 1950's Women's Culture (1950년대 여성문화장(場)에서의 밀가루음식 소비담론)

  • Kim, Mi-Hye;Chung, Hae-Kyung
    • Journal of the Korean Society of Food Culture
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    • v.25 no.6
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    • pp.639-651
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    • 2010
  • This study investigated women's magazines and women's food consumption stories of the 1950/s. That is, it attempted to comprehend the connection between the public and private aspects of food consumption as discussed in the 1950's. The public aspect of culture was investigated using the women's magazine "Yeo-won" which reflected the social and intellectual hegemony of the time. The private aspects of culture were investigated by reviewing the daily life of women though in-depth interviews. Mass media reflected the social and intellectual hegemony and indicated that a cultivated woman who supported western food was a wise mother and a good wife, and that a woman who consumed flour-based food was a reasonable and modern consumer, ahead of her time The admiration for the U.S. and its advanced civilization through free handouts of flour and powdered milk accelerated the consumption of industrialized flour-based foods such as noodles, hardtack, and steamed bread. This lead to the rigid traditional food-eating habits of boiled rice, and side dishes changed to flour-based and processed foods. That is, food represented a cultural identity.

The origination and Changes of Street Style on the Development of Rock Music (락(Rock) 음악의 발전에 따른 스트리트 스타일의 발생과 변천)

  • 정미진;정흥숙;김선화
    • Journal of the Korean Society of Costume
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    • v.52 no.5
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    • pp.173-186
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    • 2002
  • Street Style, occurred in British and America, has been expressed the character of the new generation by repeating developments and changes. Nowadays, pop music reflects the phase of society. and simultaneously it has influence on from culture to society. Rock Music was rooted in Country 8l western of America. Upon Country & Western, Afro-Americans Rhythm & Blues was added, and that was the birth of Rockabilly. Rockabilly developed to Rock'n 'roll and it started to change to various forms of Rock since 1950s. As the commercial impact and the breakthrough of teenagers emotion, the rock culture comprised the base of the youth culture. However. it formed the anti-establishment culture against the established value, accepting working class subculture. The teenager culture was affected by the Rock culture, also found relief in the opulence provided by the established generation, imitating it as it was. Simultaneously, it had a contrary aspect as anti-establishment form under the banner of revolt against languor in richness. The youth culture created street style that was escaped from high fashion, every time Rock music had changes. Since Street style was based on resistance of established culture and it fully refused vogue, it was indifferent from high fashion. The results of this study were as following. First, every time Rock music had changes, the new youth culture was concomitant with, the youth culture created street style. Secondly, rockahibilly style was characterized as diamond shaped design, embroidery, extra wide shirt collar, vivid contrast color . Rockers style was represented as metal studs, beads, denim, leather jacket, boots. Fusion of hippies style and Psychedelic, long hair, beads. worn denim were elements of headbangers style. Punks style was characterized as ripped T-shirt. rooster hair, over decorated jacket, short skirt. net or plastic T-shirt. Lastly, the firm relation between popular art and fashion was proved by examining the history of Rock Music and Street Style.