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http://dx.doi.org/10.9721/KJFST.2013.45.1.133

Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad  

Kim, Yun-Gyeong (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University)
Chon, Jung-Whan (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University)
Lee, Jae-Hoon (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University)
Kwak, Hyo-Sun (Division of Foodborne Diseases Prevention and Surveillance, National Institute of Food and Drug Safety Evaluation)
Hwang, In-Gyun (Division of Food Chemical Residues, National Institute of Food and Drug Safety Evaluation)
Seo, Kun-Ho (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University)
Publication Information
Korean Journal of Food Science and Technology / v.45, no.1, 2013 , pp. 133-136 More about this Journal
Abstract
The purpose of this study was to compare a conventional culture method and real-time PCR for the detection of Yersinia enterocolitica (Y. enterocolitica) in sausage and in vegetable salad. Food samples inoculated with Y. enterocolitica were enriched in peptone-sorbitol bile-broth, and swabs were then streaked onto cefsulodin-irgasan-novobiocin agar. Biochemical tests for suspected colonies were performed with an API 20E strip. In parallel, real-time PCR was performed, targeting the 16S rRNA gene using 1 mL of enrichment broth. In sausage, the number of positive samples detected by culture method (49 out of 60) was similar (p>0.05) with that of real-time PCR (50 out of 60). However, the number of positive samples of real-time PCR (26 out of 60) was significantly higher (p<0.05) than that of the conventional culture method (6 out of 60) in vegetable salad. Real-time PCR could be an effective screening tool for detecting Y. enterocolitica, particularly in food samples with high levels of background flora, such as a vegetable salad.
Keywords
Yersinia enterocolitica; culture method; real-time PCR; food;
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Times Cited By KSCI : 5  (Citation Analysis)
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