Comparison of Conventional Culture Method and Real-time PCR for Detection of Yersinia enterocolitica in Sausage and Vegetable Salad
![]() |
Kim, Yun-Gyeong
(KU Center for Food Safety, College of Veterinary Medicine, Konkuk University)
Chon, Jung-Whan (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University) Lee, Jae-Hoon (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University) Kwak, Hyo-Sun (Division of Foodborne Diseases Prevention and Surveillance, National Institute of Food and Drug Safety Evaluation) Hwang, In-Gyun (Division of Food Chemical Residues, National Institute of Food and Drug Safety Evaluation) Seo, Kun-Ho (KU Center for Food Safety, College of Veterinary Medicine, Konkuk University) |
1 | Cotton LN, White CH. Listeria monocytogenes, Yersinia enterocolotica, and Salmonella in dairy plant environments. J. Dairy Sci. 75: 51-57 (1992) DOI |
2 | Park SG, Choi SM, Oh YH, Choi CS. Biotype, serotype and antimicrobial susceptibility of Yersinia enterocolotica isolated from cattle in Korea. J. Korean Soc. Microbiol. 28: 453-461 (1993) |
3 | Choi CS, Park SG, Youn YD, Chung SI, Yang YT. Production of heat-stable enterotoxine and virulence-associated cultural characteristics of porcine strains of Yersinia enterocolotica and related species with or without plasmid. J. Korean Soc. Microbiol. 25: 135-146 (1990) |
4 | Park SH, Kim HJ, Kim HY. Simultaneous detection of Yersinia enterocolotica, Staphylococcus aureus, and Shigella spp. in lettuce using multiplex PCR method. J. Microbiol. Biotech. 16: 1301- 1305 (2006) |
5 | Korea Food and Drug Administration. Food code. Available from: http://safefood.kfda.go.kr/RS/food_menu.jsp. Accessed Jun. 6, 2012. |
6 | Sachse K. Specificity and performance of diagnostic PCR assays. Method. Mol. Biol. 216: 3-29 (2003) |
7 | Yang C, Jiang Y, Huang K, Zhu C, Yin Y. Application of realtime PCR for quantitative detection of Campylobacter jejuni in poultry, milk and environmental water. FEMS Immunol. Med. Mic. 38: 265-271 (2003) DOI ScienceOn |
8 | Hyeon JY, Hwang IG, Kwak HS, Park JS, Heo S, Choi IS, Park CK, Seo KH. Evaluation of an automated ELISA (VIDAS) and real-time PCR by comparing with a conventional culture method for the detection of Salmonella spp. in steamed pork and raw broccoli sprouts. Korean J. Food Sci. An. 29: 506-512 (2009) 과학기술학회마을 DOI ScienceOn |
9 | Han SR, Hyeon JY, Kim HY, Park JS, Heo S, Shin HC, Seo KH. Evaluation of conventional culture methods and validation of immunoassays for rapid detection of Listeria monocytogenes in dairy and processed foods. Korean J. Food Sci. An. 28: 616-622 (2008) 과학기술학회마을 DOI ScienceOn |
10 | Lee JH, Song KY, Hyeon JY, Hwang IG, Kwak HS, Han JA, Chung YH, Seo KH. Comparison of standard culture method and real-time PCR assay for detection of Staphylococcus aureus in processed and unprocessed foods. Korean J. Food Sci. An. 30: 410-418 (2010) 과학기술학회마을 DOI ScienceOn |
11 | Chon JW, Hyeon JY, Hwang IG, Kwak HS, Han JA, Chung YH, Song KW, Seo KH. Comparison of standard culture method and real-time PCR for detection of Vibrio parahaemolyticus in seafoods and vegetables. Korean J. Food Sci. Technol. 42: 355-360 (2010) 과학기술학회마을 |
12 | Sen K. Rapid identification of Yersinia enterocolotica in blood by the 5' nuclease PCR assay. J. Clin. Microbiol. 38: 1953-1958 (2000) |
13 | Chon JW, Hyeon JY, Hwang IG, Kwak HS, Han JA, Kim MS, Kim JH, Song KW, Seo KH. Comparison of real-time PCR and culture methods for detection of Campylobacter jejuni in various foods. Korean J. Food Sci. Technol. 43: 119-123 (2011) 과학기술학회마을 DOI ScienceOn |
14 | Malorny B, Paccassoni E, Fach P, Bunge C, Martin A, Helmuth R. Diagnostic real-time PCR for detection of Salmonella in food. Appl. Environ. Microb. 70: 7064-7052 (2004) |
15 | United States Department of Agriculture/Food Safety and Inspection Service. Microbiology Laboratory Guidebook. Available from: http://www.fsis.usda.gov/Science/Microbiological_Lab_Guidebook/ index.asp. Accessed Jun. 6, 2012. |
![]() |