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Analysis of Iridoid Glycoside and GABA Content in the Roots of the Rehmannia glutinosa Cultivars (지황 품종별 뿌리에서 Iridoid 배당체와 GABA 분석)

  • Lee, Sang Hoon;Yoon, Jeong Su;Kim, Jae Kwang;Park, Chun Geun;Chang, Jae Ki;Kim, Yeon Bok
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.3
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    • pp.146-151
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    • 2017
  • Background: Rehmannia glutinosa is a perennial herb belonging to the family Scrophulariaceae. Its roots have been utilized as a traditional medicine. The aim of this study was to elucidate the basic information of the roots of the R. glutinosa cultivars and their utilization. Methods and Results: The roots of R. glutinosa cultivars were harvested in the end of March. The two iridoid glycosides, aucubin and catalpol, were analyzed by liquid chromatography/mass spectrometry (LC/MS), whereas ${\gamma}-aminobutyric$ acid (GABA) was analyzed by gas chromatography/mass spectrometry (GC/MS). The aucubin content was the highest in the Dakang cultivar, whereas no aucubin was detected in the five cultivars. All cultivars had more than 12 mg/g catalpol content, and the maximum catalpol content was found in Jihwang 1. The GABA content was the highest in Suwon 1, and it was 40 times more than that in the Yeongang cultivar. Conclusions: The highest aucubin, catapol and GABA contents were detected in the Dakang, Jihwang 1, and Suwon 1, cultivars respectively. This study provides the crucial information regarding the versatile utilization and pedigree selection of R. glutinosa cultivars.

Differential Symbiotic Response of Phage-typed Strains of Bradyrhizobium japonicum with Soybean Cultivars

  • Appunu Chinnaswamy;Dhar Banshi
    • Journal of Microbiology
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    • v.44 no.3
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    • pp.363-368
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    • 2006
  • In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.

A Study on the Korean Native Woody Plants of Trees in the North American Landscape (한국자생으로서 미국 및 카나다에서 재배되고 있는 조경수목(교목) 에 관한 연구)

  • 심경구;서병기
    • Journal of the Korean Institute of Landscape Architecture
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    • v.22 no.4
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    • pp.95-117
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    • 1995
  • This study was carried out to investigate Korean native trees in the America landscape through arboretums, the plant inventory of America, computer files for accessions from Korea, source lists of plants and seeds, and seed and plant nursery company. The results were as follows; 1. Korean native trees introduced in America and Canada were 119 species, 116 species were being cultivated in arbrids were being sold in nurseries, new cultivars were selected from 32 species, and hybrids were bred from 5 species. 2. Abies koreana, Malus baccata, and Stewartia koreana were being cultivated in many arboretums and sold in many nurseries. Also there were new hybrids as well as new cultivars. 3. 25 species including Albizzia julibrissin were being cultivated in many arboretums and sold in many nurseries. Also there were new hybrids. 4. Ilex integra and Ilex rotunda were being cultivated in many arboretums and sold in many nurseries. There were no new cultivars, but there were new hybrids. 5. 44 species including Abies holophylla were being cultivated in many arboretums and sold in many nurseries. There were no new cultivars or hybrids. 6. Populus tremula var. davidiana was being cultivated in many arboretums. There were new cultivars of 'Erecta', 'Gigas', 'Pendula', but no new hybrids. They were not sold by any nurseries. 7. 41 species including Abies nephrolepsis were being cultivated in many arboretums. But they were not sold by any nurseries. They didn't have any new cultivars or hybrids. 8. Diospyros kaki 'Kyungsan Bansi', Elaeocarpus sylvestris var. ellipticus, and Pyrus serotina 'Dankae' were not being cultivated in arboretums in America but some nurseries were selling those species as well as new cultivars. 9. Only 3 species (Berchemia berchemiaefolia, Carpinus eximia, and Chionanthus retusus var. coreana which are native Korean plants) were not introduced in America or Canada.

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Variation of Nitrogen Use Efficiency and Its Relationships with Growth Characteristics in Rice Cultivars

  • Lee, Seung-Hun;Lee, Ho-Jin;Chung, Ji-Hoon;Cho, Young-Chul;Lee, Jae-Hong;Kim, Hee-Dong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.2
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    • pp.89-93
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    • 2004
  • This experiment was conducted to investigate the variation of nitrogen use efficiency, nitrogen uptake efficiency, physiological utilization efficiency and their relationships with growth characteristics in the 28 Korean rice cultivars. Nitrogen use efficiency of 28 rice cultivars was 47.74, nitrogen uptake efficiency was 0.71, and physiological utilization efficiency was 68.76 in average. Nitrogen use efficiency of rice cultivars had low variation ranged from 44.09 to 51.91, but nitrogen uptake efficiency were relatively high variation from 0.51 to 0.90, and physiological utilization efficiency was from 51.71 to 94.26. The high efficient group in nitrogen uptake efficiency whose value was calculated above 0.80 included Daeanbyeo, Seojinbyeo, Ansungbyeo, Dongjinbyeo, and Hwaanbyeo, while the low efficient group with below 0.60 was Kwanganbyeo, Sampyeongbyeo, Soorabyeo, and Hwasungbyeo. Hwasungbyeo, Sampyeongbyeo, Soorabyeo for physiological utilization efficiency were more efficient cultivars, while Daeanbyeo, Seojinbyeo, Ansungbyeo were less efficient cultivars. Nitrogen uptake efficiency had positive correlation coefficients between dry matter weight of plant ($0.842^{**}$), leaf area index ($0.761^{**}$), and leaf nitrogen content ($0.599^{**}$), respectively. Therefore, the dry matter weight of rice plant and leaf area index was important characters to evaluate nitrogen uptake efficiency in rice cultivars. Also, more efficient cultivar in nitrogen uptake had higher chlorophyll meter value, which was appeared dark green color.

Comparison of Physicochemical and Sensory Characteristics of Soybean Sprouts from Different Cultivars (나물콩 품종별 콩나물의 물리화학적 및 관능적 특성 비교)

  • Kim, Sung-Soo;Hong, Hee-Do;Lee, Jin-Yeol;Choi, Hee-Don
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.207-212
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    • 2000
  • Physicochemical and sensory characteristics of soybean sprouts from domestic and foreign soybean cultivars were investigated. 100-seed weights of domestic cultivars were much larger than that of Canadian cultivar and recommended cultivars were larger than traditional cultivars. Water absorption of soybeans increased sharply to initial 6 hrs during steeping, and did not change much thereafter. In all soybean cultivars 16 amino acids were detected and the contents of Asp, Glu, Lys and Arg were more than 50% of total amino acid contents. All soybean cultivars showed some difference in composition of fatty acids and the content of linoleic acid was highest. The contents of K and P were high and those of Fe and Zn were low. The compression force of hypocotyl after cultivation was the highest in Eunha and the lowest in Junjeori and the compression force of cotyledon was the highest in Danyeop and the lowest in Canadian cultivar. Qualitative discription analysis for cooked soybean sprouts and soups showed significant difference in 6 characteristics of yellowness of cotyledon, size of cotyledon and so on.

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Screening of Resistance Cultivar to Clubroot Caused by Plasmodiophora brassicae for Organic Cultivation of Chinese Cabbage

  • Kim, Min-Jeong;Shim, Chang-Ki;Kim, Yong-Ki;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Lee, Min-Ho;Jee, Hyeong-Jin
    • Research in Plant Disease
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    • v.18 no.2
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    • pp.123-128
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    • 2012
  • We investigated the resistance of 50 commercial Chinese cabbage cultivars against clubroot disease caused by Plasmodiophora brassicae in the three difference fields, Suwon, Hwacheon, and Pyeongchang. Wilting symptom on Chinese cabbage was first observed at 15 days after transplanting in Pyeongchang and Hwacheon, while disease symptoms appeared later in Suwon after the rainy season. Among 50 cultivars, eight cultivars, SC26, SC29, SC30, SC31, SC34, SC46, SC47 and SC50 showed highly susceptible symptoms like wilting and heavy root galls in all three fields. Meanwhile, seven cultivars such as SC05, SC06, SC07, SC09, SC11, SC17, and SC36 showed moderate resistance with delayed wilting and few root galls. Only two cultivars, Chuwol (CB22) and Gohyangssam (CB23) were highly resistant to clubroot disease until the harvest season in all of the three fields. These two commercial cultivars may be considered as candidate cultivars for cultivation of organic Chinese cabbage in Suwon, Hwacheon, and Pyeongchang.

Pathotype Classification of Plasmodiophora brassicae Isolates Using Clubroot-Resistant Cultivars of Chinese Cabbage

  • Kim, Hun;Jo, Eun Ju;Choi, Yong Ho;Jang, Kyoung Soo;Choi, Gyung Ja
    • The Plant Pathology Journal
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    • v.32 no.5
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    • pp.423-430
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    • 2016
  • Clubroot disease caused by Plasmodiophora brassicae is one of the most serious diseases in Brassica crops worldwide. In this study, the pathotypes of 12 Korean P. brassicae field isolates were determined using various Chinese cabbage including 22 commercial cultivars from Korea, China, and Japan, and 15 inbred lines. All P. brassicae isolates exhibited the typical clubroot disease on non-clubroot resistant cultivar, indicating that the isolates were highly pathogenic. According to the reactions on the Williams' hosts, the 12 field isolates were initially classified into five races. However, when these isolates were inoculated onto clubroot-resistant (CR) cultivars of Chinese cabbage, several isolates led to different disease responses even though the isolates have been assigned to the same race by the Williams' host responses. Based on the pathogenicity results, the 12 field isolates were reclassified into four different groups: pathotype 1 (GN1, GN2, GS, JS, and HS), 2 (DJ and KS), 3 (HN1, PC, and YC), and 4 (HN2 and SS). In addition, the CR cultivars from Korea, China, and Japan exhibited distinguishable disease responses to the P. brassicae isolates, suggesting that the 22 cultivars used in this study, including the non-CR cultivars, are classified into four different host groups based on their disease resistance. Combining these findings, the four differential hosts of Chinese cabbage and four pathotype groups of P. brassicae might provide an efficient screening system for resistant cultivars and a new foundation of breeding strategies for CR Chinese cabbage.

Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato

  • Lee, Hyung Jin;Kim, Boyoung;Bae, Chungyun;Kang, Won-Hee;Kang, Byoung-Cheorl;Yeam, Inhwa;Oh, Chang-Sik
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.730-736
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    • 2015
  • Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.

Resistance of Cowpea Cultivars to Meloidogyne arenaria and M. incognita in Korea (동부품종의 국내 분포 뿌리혹선충 저항성 검정)

  • Kang, Heonil;Jun, Taehwan;Kwon, Soonwook;Kim, Suntae;Kang, Hangwon;Kim, Yongchul;Kim, Donggeun;Ko, Hyoungrai;Choi, Insoo
    • Journal of Environmental Science International
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    • v.27 no.12
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    • pp.1241-1247
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    • 2018
  • This study was conducted to evaluate resistance of 10 different cowpea cultivars which were introduced from the United States against two major root-knot nematodes, Meloidogyne arenaria and M. incognita, that affect greenhouse crop cultivation in Korea. The results showed that the resistance of the tested cultivars to the root-knot nematodes varied with the cultivar. Texas Cream 40 showed moderate resistance to M. arenaria whereas the other cultivars were susceptible. Purple Hull Pinkeye and Texas Pinkeye Purple Hull were found to be resistant to M. incognita, whereas Mississippi Silver showed moderate resistance and the other cultivars were susceptible. As the cultivars exhibit resistance to M. arenaria and M. incognita, such cultivars resistant to root-knot nematodes should be considered during the cultivation of cowpea as a green manure crop.

Variation in the Resistance of Japanese Soybean Cultivars to Phytophthora Root and Stem Rot during the Early Plant Growth Stages and the Effects of a Fungicide Seed Treatment

  • Akamatsu, Hajime;Kato, Masayasu;Ochi, Sunao;Mimuro, Genki;Matsuoka, Jun-ichi;Takahashi, Mami
    • The Plant Pathology Journal
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    • v.35 no.3
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    • pp.219-233
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    • 2019
  • Soybean cultivars susceptible to Phytophthora root and stem rot are vulnerable to seed rot and damping-off of seedlings and young plants following an infection by Phytophthora sojae. In this study, the disease responses of Japanese soybean cultivars including currently grown main cultivars during the early growth stages were investigated following infections by multiple P. sojae isolates from Japanese fields. The extent of the resistance to 17 P. sojae isolates after inoculations at 14, 21, and 28 days after seeding varied significantly among 18 Japanese and two US soybean cultivars. Moreover, the disease responses of each cultivar differed significantly depending on the P. sojae isolate and the plant age at inoculation. Additionally, the treatment of 'Nattosyo-ryu' seeds with three fungicidal agrochemicals provided significant protection from P. sojae when plants were inoculated at 14-28 days after seeding. These results indicate that none of the Japanese soybean cultivars are completely resistant to all tested P. sojae isolates during the first month after sowing. However, the severity of the disease was limited when plants were inoculated during the later growth stages. Furthermore, the protective effects of the tested agrochemicals were maintained for at least 28 days after the seed treatment. Japanese soybean cultivars susceptible to Phytophthora root and stem rot that are grown under environmental conditions favorable for P. sojae infections require the implementation of certain practices, such as seed treatments with appropriate agrochemicals, to ensure they are protected from P. sojae during the early part of the soybean growing season.