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Differential Symbiotic Response of Phage-typed Strains of Bradyrhizobium japonicum with Soybean Cultivars  

Appunu Chinnaswamy (Microbial Genetics Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University)
Dhar Banshi (Microbial Genetics Laboratory, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University)
Publication Information
Journal of Microbiology / v.44, no.3, 2006 , pp. 363-368 More about this Journal
Abstract
In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.
Keywords
B. japonicum; cultivars interaction; phage types; soybean;
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1 Barnet, Y.M. 1979. Properties of Rhizobium trifolii isolates surviving exposure to specific bacteriophages. Can. J. Microbiol. 25, 979-986   DOI   ScienceOn
2 Dhar, B., K.K. Upadhyay, and R.M. Singh. 1993. Isolation and characterization of bacteriophages specific for Rhizobium leguminosarum biovar phaseoli. Can. J. Microbiol. 30, 775-779
3 Israel, D.W, J.N. Mathis, WM. Barbour, and G.H. Elkan. 1986. Symbiotic effectiveness and host-strain interactions of Rhizobium fredii USDA191 on different soybean cultivars. Appl. Environ. Microbiol. 51, 898-903
4 Kleczkowska, J. 1971. Genetical changes in Rhizobium bacteria and in their bacteriophages during co-existence. Plant Soil. (Special issue) 47-63
5 Shutsrirung, A., S. Thampibool, A. Bhromsiri, K. Senoo, S. Tajima, and M. Hisamatsu. 2002. Symbiotic efficiency and compatibility of native rhizobia in northern Thailand with different soybean cultivars. II. Laboratory experiment using native isolates from irrigated soybean-growing areas, Soil Sci. Plant Nutri. 48, 501-510   DOI   ScienceOn
6 Date, R.A. and R.J. Roughley. 1977. Preparation of legume inoculations, p. 243-276. In R.WF. Hardy and WS. Silver (eds.), A Treatise on dinitrogen fixation-section III: Biology. John Wiley and Sons, New York
7 Dhar, B. and B. Kumar. 1998. Occurrence and characteristics of rhizobiophages in pigeon pea fields. Indian J. Microbiol. 38, 141-144
8 Vincent, lM. 1970. A manual for the practical study of root nodule bacteria- IBH Hand Book, p. 1-164. Blackwell Scientific Publications, Oxford and Edinburgh
9 Dhar, B. and K. Ramkrishna. 1987. Morphology and general characteristics of phages of chickpea rhizobia. Arch. Microbiol. 147, 121-125   DOI
10 Hardarson, G., F. Zapata, and S.K.A. Danso. 1984. Effect of plant genotypes and nitrogen fertilizer on symbiotic nitrogen fixation by soybean cultivars. Plant Soil 82, 397-405   DOI
11 Patel, J.J. 1978. Symbiotic effectiveness of phage resistant mutants of two strains of lotus strain of lotus rhizobia. Plant Soil. 49, 251-257   DOI
12 Caldwell, B.W and H.G. Vest. 1977. Genetic aspects of nodulation and dinitrogen fixation by legumes: The macro-symbiont, p.1-557. In R.W.F. Hardy and W.S. Silver (eds.), A Treatise on dinitrogen fixation-Section III: Biology. John Wiley and Sons, New York
13 Palmer, R.G. and T.C. Kilen. 1987. Quantitative genetics and cytogenetics, p. 135-147. In J.R. Wilcox (ed.), soybeans: Improvements, production and uses. American Society of Agronomy, WI
14 Kucey, R.M.V., P. Snitwongse, P. Chaiwanakupt, P. Wadisirisuk, C. Siripaipool, T. Arayangkool, N. Boonkerd, and R.J. Rennie. 1988. Nitrogen fixation ($^{15}N$ dilution) with soybeans under Thai field conditions. I. Developing protocols for screening Bradyrhizobium japonicum strains. Plant Soil 108, 33-41   DOI
15 Sornasegaran, P. and H.J. Hoben. 1994. Handbook for Rhizobia, Methods in Legume-Rhizobium Technology, p.1-450. Springer-Verlag, Heidelberg, Germany
16 Dhar, B., B.D. Singh, R.B. Singh, J.S. Srivastava, and R.M. Singh. 1980. Seasonal incidence of rhizobiophages in soil around Varanasi. Indian J. Exptl. Biol. 18, 1168-1170
17 Singleton, P.W. and J.W. Tavares. 1986. Inoculation response of legumes in relation to the number and effectiveness of indigenous Rhizobium populations. Appl. Environ. Microbiol. 51, 1013-1018
18 Hunt, P.G., S.E. Sojka, T.A. Mathery, and A.G. Wollum II. 1985. Soybean response to Rhizobium japonicum strain, row orientation and irrigation. Agron. J. 77, 720-725   DOI
19 Zhang, F. and D.L. Smith. 2002. Interorganismal signaling in suboptimum envirouments: The legume rhizobia symbiosis. Adv. Agron. 76, 125-157   DOI
20 Sims, A.P., B.F. Folkes, D.J. Barber, and D. Walls. 1983. The relative importance of nitrogen assimilation and dinitrogen fixation in the economy of the pea crop, p. 159-174. In D.G. Jones, and D.R. Davies (eds.), Temperate Legumes Physiology, genetics and nodulation. Pitman Books Ltd., London
21 Pate, J.S. and C.A. Atkins. 1983. Nitrogen uptake, transport and utilization, p. 245-298. In W.J. Broughton (ed.), Nitrogen fixation-Legumes. Clarendon Press, Oxford
22 Israel, D.W. 1981. Cultivar and Rhizobium strain effects on nitrogen fixation and remobilization by soybeans. Agron. J. 73, 509-516   DOI
23 Dhar, B., B.D. Singh, R.B. Singh, J.S. Srivastava, Y.P. Singh, and R.M. Singh. 1979. Occurrence and distribution of rhizobiophages in Indian soils. Acta Microbiol. Pol. 28, 319-324