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http://dx.doi.org/10.7235/hort.2015.15025

Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato  

Lee, Hyung Jin (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University)
Kim, Boyoung (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University)
Bae, Chungyun (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University)
Kang, Won-Hee (Department of Plant Science, Seoul National University)
Kang, Byoung-Cheorl (Department of Plant Science, Seoul National University)
Yeam, Inhwa (Horticulture and Breeding Major, School of Bioresource Sciences, Andong National University)
Oh, Chang-Sik (Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University)
Publication Information
Horticultural Science & Technology / v.33, no.5, 2015 , pp. 730-736 More about this Journal
Abstract
Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.
Keywords
Genotypes; HRM; marker-assisted selection; SNP marker; TSWV;
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1 Saidi, M. and S. D. Warade. 2008. Tomato breeding for resistance to Tomato spotted wilt virus (TSWV): an overview of conventional and molecular approaches. Czech J. Genet. Plant Breed. 44:83-92.
2 Shi, A., R. Vierling, R. Grazzini, P. Chen, H. Caton, and D. Panthee. 2011. Identification of molecular markers for Sw-5 gene of tomato spotted wilt virus resistance. Am. J. Biotechnol. Mol. Sci. 1:8-16.   DOI
3 Smiech, M., Z. Rusinowski, S. Malepszy, and K. Niemirowicz- Szczytt. 2000. New RAPD markers of Tomato spotted wilt virus (TSWV) resistance in Lycopersicon esculentum Mill. Acta Physiol. Plantarum 22:299-303.   DOI
4 Spassova, M. I., T. W. Prins, R. T. Folkertsma, R. M. Klein- Lankhorst, J. Hille, R. W. Goldbach, and M. Prins. 2001. The tomato gene Sw5 is a member of the coiled coil, nucleotide binding, leucine-rich repeat class of plant resistance genes and confers resistance to TSWV in tobacco. Mol. Breed. 7:151-161.   DOI
5 Stevens, M., E. Lamb, and D. Rhoads. 1995. Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomatoes using RAPD and RFLP analyses. Theor. Appl. Genet. 90:451-456.
6 Stevens, M., S. Scott, and R. Gergerich. 1991. Inheritance of a gene for resistance to Tomato spotted wilt virus (TSWV) from Lycopersicon peruvianum Mill. Euphytica 59:9-17.
7 Wu, S.-B., M. G. Wirthensohn, P. Hunt, J. P. Gibson, J, and M. Sedgley. 2008. High resolution melting analysis of almond SNPs derived from ESTs. Theor. Appl. Genet. 118, 1-14.   DOI
8 Yang, W., X. Bai, E. Kabelka, C. Eaton, S. Kamoun, E. van der Knaap, and D. Francis. 2004. Discovery of single nucleotide polymorphisms in Lycopersicon esculentum by computer aided analysis of expressed sequence tags. Mol. Breed. 14:21-34.   DOI
9 Brommonschenkel, S. H., A. Frary, A. Frary, A, and S. D. Tanksley. 2000. The broad-spectrum tospovirus resistance gene Sw-5 of tomato is a homolog of the root-knot nematode resistance gene Mi. Mol. Plant-Microbe Interact. 13:1130-1138.   DOI
10 Dianese, E. C., M. E. N, de Fonseca, R. Goldbach, R. Kormelink, A. K. Inoue-Nagata, R. O. Resende, and L. S. Boiteux. 2010. Development of a locus-specific, co-dominant SCAR marker for assisted-selection of the Sw-5 (Tospovirus resistance) gene cluster in a wide range of tomato accessions. Mol. Breed. 25:133-142.   DOI
11 Finlay, K. 1953. Inheritance of spotted wilt resistance in the tomato II. Five genes controlling spotted wilt resistance in four tomato types. Aust. J. Biol. Sci. 6:153-163.
12 Foolad, M. R. 2007. Genome mapping and molecular breeding of tomato. Int. J. Plant Genomics 2007:64358.
13 Foolad, M. R. and D. R. Panthee. 2012. Marker-assisted selection in tomato breeding. Crit. Rev. Plant Sci. 31:93-123.   DOI
14 Foolad, M. R. and A. Sharma. 2004. Molecular markers as selection tools in tomato breeding. Acta Hortic. 695:225-240.
15 Garland, S., M. Sharman, D. Persley, and D. McGrath. 2005. The development of an improved PCR-based marker system for Sw-5, an important TSWV resistance gene of tomato. Crop Pasture Sci. 56:285-289.   DOI
16 German, T. L., D. E. Ullman, and J. W. Moyer. 1992. Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu. Rev. Phytopathol. 30:315-348.   DOI
17 Langella, R., M. Ercolano, L. Monti, L. Frusciante, and A. Barone. 2004. Molecular marker assisted transfer of resistance to TSWV in tomato elite lines. J. Hortic. Sci. Biotechnol. 79:806-810.   DOI
18 Yang, W., S. Miller, J. Scott, J. Jones, and D. Francis. 2005. Mining tomato genome sequence databases for molecular markers: application to bacterial resistance and marker assisted selection. Acta Hortic. 695:241-250.
19 Giancola, S., H. I. McKhann, A. Berard, C. Camilleri, S. Durand, S, P. Libeau, F. Roux, X. Reboud, I. G. Gut, and D. Brunel. 2006. Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploid plants. Theor. Appl. Genet. 112:1115-1124.   DOI
20 Labate, J. A. and A. M. Baldo. 2005. Tomato SNP discovery by EST mining and resequencing. Mol. Breed. 16:343-349.   DOI
21 Murray, M. and W. F. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321-4326.   DOI
22 Nasu, S., J. Suzuki, R. Ohta, K. Hasegawa, R. Yui, N. Kitazawa, L. Monna, and Y. Minobe. 2002. Search for and analysis of single nucleotide polymorphisms (SNPs) in rice (Oryza sativa, Oryza rufipogon) and establishment of SNP markers. DNA Res. 9:163-171.   DOI
23 Price, D. L., F. D. Memmott, J. W. Scott, S. M. Olson, and M. R. Stevens. 2007. Identification of molecular markers linked to a new Tomato spotted wilt virus resistance source in tomato. Tomato Genet. Coop. 57:35-36.
24 Rafalski, A. 2002. Applications of single nucleotide polymorphisms in crop genetics. Curr. Opin. Plant Biol. 5:94-100.   DOI
25 Rosello, S., B. Ricarte, M. J. Diez, and F. Nuez. 2001. Resistance to Tomato spotted wilt virus introgressed from Lycopersicon peruvianum in line UPV 1 may be allelic to Sw-5 and can be used to enhance the resistance of hybrids cultivars. Euphytica 119:357-367.   DOI