• The Plant Pathology Journal
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• 제31권4호
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• pp.371-378
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• 2015

Surveys of yellowing viruses in plastic tunnels and in open field crops of melon (Cucumis melo cultivar catalupo), oriental melon (C. melo cultivar oriental melon), and cucumber (C. sativus) were carried out in two melon-growing areas in 2014, Korea. Severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. The symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thickening of older leaves. RT-PCR analysis using total RNA extracted from diseased leaves did not show any synthesized products for four cucurbit-infecting viruses; Beet pseudo-yellows virus, Cucumber mosaic virus, Cucurbit yellows stunting disorder virus, and Melon necrotic spot virus. Virus identification using RT-PCR showed Cucurbit aphid-borne yellows Virus (CABYV) was largely distributed in melon, oriental melon and cucumber. This result was verified by aphid (Aphis gossypii) transmission of CABYV. The complete coat protein (CP) gene amplified from melon was cloned and sequenced. The CP gene nucleotide and the deduced amino acid sequence comparisons as well as phylogenetic tree analysis of CABYV CPs showed that the CABYV isolates were undivided into subgroups. Although the low incidence of CABYV in infections to cucurbit crops in this survey, CABYV may become an important treat for cucurbit crops in many different regions in Korea, suggesting that CABYV should be taken into account in disease control of cucurbit crops in Korea.

• 농업과학연구
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• 제49권1호
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• pp.121-128
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• 2022

Fusarium wilt disease caused by Fusarium species is a major problem affecting cultivated cucurbit plants worldwide. Fusarium species are well-known soil-borne pathogenic fungi that cause Fusarium wilt disease in several cucurbit plants. In this study, we aimed to identify and classify pathogenic Fusarium species from cultivated Korean cucurbit plants, specifically watermelon and cucumber. Thirty-six Fusarium isolates from different regions of Korea were obtained from the National Institute of Horticulture and Herbal Science Germplasm collection. Each isolate was morphologically and molecularly identified using an internal transcribed spacer of ribosomal DNA, elongation factor-1α, and the beta-tubulin gene marker sequence. Fusarium species that infect the cucurbit plant family could be divided into three groups: Fusarium oxysporum (F. oxysporum), Fusarium solani (F. solani), and Fusarium equiseti (F. equieti). Among the 36 isolates examined, six were non-pathogenic (F. equiseti: 15-127, F. oxysporum: 14-129, 17-557, 17-559, 18-369, F. solani: 12-155), whereas 30 isolates were pathogenic. Five of the F. solani isolates (11-117, 14-130, 17-554, 17-555, 17-556) were found to be highly pathogenic to both watermelon and cucumber plants, posing a great threat to cucurbit production in Korea. The identification of several isolates of F. equiseti and F. oxysporum, which are both highly pathogenic to bottle gourd, may indicate waning resistance to Fusarium species infection.

• 식물병연구
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• 제29권1호
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• pp.52-59
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• 2023

Cucurbit chlorotic virus (박과퇴록황화바이러스, CCYV)는 수박, 오이 등의 박과작물에 영향을 미치는 식물바이러스로 담배가루이에 의해 전반된다. 2018년 충북 오이에서 처음 진단된 이후 경상도 등 다른 지역에서도 보고되고 있다. 2020년 충북 진천, 음성지역의 박과작물 온실의 황화 바이러스병 조사에서 멜론, 수박, 잡초 등 채집시료 총 79시료에 대한 CCYV 특이 프라이머를 이용한 reverse transcription-polymerase chain reaction 결과 멜론 4개의 시료에서 CCYV가 확인되었다. 3점은 CCYV 단독감염, 1점는 Cucurbit aphid borne yellows virus와 Watermelon mosaic virus 복합감염으로 나타났다. 4개의 CCYV ES 분리주로부터 RNA 1, 2의 전체게놈을 얻었고, 이전에 GenBank에 보고된 CCYV 분리주들과 염기서열을 분석하였다. MEGA를 이용하여 계통분석결과 ES 분리주들은 하나의 그룹으로 나타났고, 중국 분리주들과 밀접한 관련이 있었다. ES 분리주들 유전적 다양성이 낮은 것으로 나타났고, 일반적으로 CCYV은 기주 및 지리적 기원에 따라 유전적 다양성이 거의 없었다. CCYV는 박과작물 생산에 심각한 위협이 될 가능성이 있다. 수박, 잡초 등을 포함한 다양한 CCYV 분리주에 대한 병원성, 가루이 전염 등의 특성에 대한 추가적인 연구가 필요하다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.150.1-150
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• 2003

A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses： cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.

• 대한화학회지
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• 제49권1호
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• pp.112-116
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• 2005

• 환경생물
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• 제38권3호
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• pp.486-495
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• 2020

토마토와 박과류채소의 유기농산물을 생산하기 위하여 무농약으로 재배하는 시설하우스에서 최근 문제되는 병해를 조사한 결과 토마토 잎곰팡이병과 토마토반점위조바이러스병, 토마토퇴록바이러스병, 박과류 쥬키니황화모자이크바이러스병, 박과류 흰가루병 등의 피해가 심한 것으로 조사되었다. 농약을 사용하여 일반재배를 하는 시설하우스에 비해 무농약재배 하우스에서 재배되는 작물에서 병 발생 시기가 더 빠르고 병 발생도 심한 것으로 나타났으며, 총채벌레와 진딧물 등에 의해 매개되는 바이러스병 피해가 심한 것으로 조사되었다. 따라서 우수한 유기농산물을 생산하기 위해서는 병해충방제를 효율적으로 하는 것이 중요하며, 병 발생 피해를 최소화기 위해서는 포장위생과 물리적 차단, 유기농업자재나 미생물제 등을 종합적으로 활용하여 예방적으로 방제하는 것이 가장 중요하다.

• Mass Spectrometry Letters
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• 제7권1호
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• pp.16-20
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• 2016

Characterization of intact protein structures in the gas phase using electrospray ionization combined with ion mobility mass spectrometry has become an important tool of research. However, the biophysical properties that govern the structures of protein ions in the gas phase remain to be understood. Here, we investigated the impact of host-guest complexation of ubiquitin (Ubq) with macrocyclic host molecules, cucurbit[n]urils (CB[n]s, n = 6, 7), on its structure in the gas phase. We found that CB[n] complexation induces the formation of compact Ubq ions. Both CB[6] and CB[7] exhibited similar effects despite differences in their binding properties in solution. In addition, CB[n] attachment prevented Ubq from unfolding by collisional activation. Based on the experimental results, we suggest that CB[n]s prevent unfolding of Ubq during transfer to the gas phase to promote the formation of compact protein ions. Furthermore, interaction with positively charged residues per se is suggested to be the most important factor for the host-guest complexation effect.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.514-521
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• 2022

We report the effect of pH on the supramolecular complexation of two biothiols, viz., homocysteine (Hcy) and cysteine (Cys), with cucurbit[7]uril (CB[7]). Under basic pH conditions, Cys did not complex with CB[7], whereas Hcy efficiently complexed with CB[7], as confirmed by ¹H NMR spectroscopy and Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid), DTNB) assay. ¹H NMR and Raman spectroscopic studies revealed that, in the absence of CB[7], Hcy auto-oxidized slowly (~36 h) to homocystine (HSSH) under basic pH conditions. However, the rate of Hcy oxidation increased by up to 150 fold in the presence of CB[7], as suggested by the DTNB assay. Thus, supramolecular complexation under basic pH conditions led to the formation of a HSSH-CB[7] complex, and not Hcy-CB[7]. The results indicate that Hcy is rapidly oxidized to HSSH under the catalysis of CB[7], which acts as a reaction chamber, in basic pH conditions. Our studies suggest that Hcy concentration, a risk factor for cardiovascular disease, can be selectively and more easily quantified by supramolecular complexation with CB [7].

• The Plant Pathology Journal
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• 제38권4호
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• pp.296-303
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• 2022

The cucurbit powdery mildew (CPM) caused by different fungal species is a major concern for cucurbit crops around the world. In Argentina CPM constitutes the most common and damaging disease for cucurbits, especially for squash crops (Cucurbita moschata). The present study displays initial insights into the knowledge of the disease in western Argentina, including the determination of the prevalent species causing CPM, as well as the evaluation of the resistance of squash cultivars and breeding lines. Fungal colonies were isolated from samples collected in Mendoza province, Argentina. A field trial was also performed to assess the resistance of five squash accessions, including commercial cultivars and breeding lines. The severity of CPM was analyzed and epidemiological models were built based on empirical data. The morphological determinations and analysis with specific molecular markers confirmed Podosphaera xanthi as the prevalent causal agent of CPM in Mendoza. The results od the field trial showed differences in the resistance trait among the squash accessions. The advanced breeding line BL717/1 showed promising results as source of CPM resistance for the future development of open pollinated resistant cultivars, a crucial tool for an integrative control of the disease.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2001년도 임시총회 및 추계학술대회연구발표회(한국결정학회)
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• pp.19-19
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• 2001