Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Phylogenetic Analysis of Cucurbit Chlorotic Yellows Virus from Melon in 2020 in Chungbuk, Korea

2020년 충북지역 멜론에서 발생한 Cucurbit Chlorotic Yellows Virus의 계통분석

  • Taemin Jin (Department of Plant Medicine, Chungbuk National University) ;
  • Hae-Ryun Kwak (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hong-Soo Choi (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Byeongjin Cha (Department of Plant Medicine, Chungbuk National University) ;
  • Jong-Woo Han (Department of Plant Medicine, Chungbuk National University) ;
  • Mikyeong Kim (Department of Plant Medicine, Chungbuk National University)
  • 진태민 (충북대학교 식물의학과) ;
  • 곽해련 (국립농업과학원 작물보호과) ;
  • 최홍수 (국립농업과학원 작물보호과) ;
  • 차병진 (충북대학교 식물의학과) ;
  • 한종우 (충북대학교 식물의학과) ;
  • 김미경 (충북대학교 식물의학과)
  • Received : 2023.02.21
  • Accepted : 2023.03.03
  • Published : 2023.03.31
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Abstract

Cucurbit chlorotic yellows virus (CCYV) is a plant virus that causes damage to cucurbit crops such as watermelon and cucumber, and is transmitted by an insect vector known as the whitefly. Since CCYV was first detected on cucumber in Chungbuk in 2018, it has been reported in other areas including Gyeongsang in Korea. In 2020, we performed field surveys of yellowing diseases in the greenhouses growing melon and watermelon in Chungbuk (Jincheon and Eumseong). Reverse transcription-polymerase chain reaction analysis of 79 collected samples including melon, watermelon, and weeds resulted in detection of CCYV in 4 samples: Three samples were singly infected with CCYV and one samples was mixed infected with CCYV, Cucurbit aphid borne yellows virus, and Watermelon mosaic virus. The complete genome sequences of the four collected CCYV melon isolates (ES 1-ES 4) were determined and genetically compared with those of previously reported CCYV isolates retrieved from GenBank. Phylogenetic analyses of RNA 1 and 2 sequences revealed that four ES isolates were clustered in one group and closely related to the CCYV isolates from China. The analysis also revealed very low genetic diversity among the CCYV ES isolates. In general, CCYV isolates showed little genetic diversity, regardless of host or geographic origins. CCYV has the potential to pose a serious threat to melon, watermelon, and cucumber production in Korea. Further studies are needed to examine the pathogenicity and transmissibility of CCYV in weeds and other cucurbits including watermelon.

Cucurbit chlorotic virus (박과퇴록황화바이러스, CCYV)는 수박, 오이 등의 박과작물에 영향을 미치는 식물바이러스로 담배가루이에 의해 전반된다. 2018년 충북 오이에서 처음 진단된 이후 경상도 등 다른 지역에서도 보고되고 있다. 2020년 충북 진천, 음성지역의 박과작물 온실의 황화 바이러스병 조사에서 멜론, 수박, 잡초 등 채집시료 총 79시료에 대한 CCYV 특이 프라이머를 이용한 reverse transcription-polymerase chain reaction 결과 멜론 4개의 시료에서 CCYV가 확인되었다. 3점은 CCYV 단독감염, 1점는 Cucurbit aphid borne yellows virus와 Watermelon mosaic virus 복합감염으로 나타났다. 4개의 CCYV ES 분리주로부터 RNA 1, 2의 전체게놈을 얻었고, 이전에 GenBank에 보고된 CCYV 분리주들과 염기서열을 분석하였다. MEGA를 이용하여 계통분석결과 ES 분리주들은 하나의 그룹으로 나타났고, 중국 분리주들과 밀접한 관련이 있었다. ES 분리주들 유전적 다양성이 낮은 것으로 나타났고, 일반적으로 CCYV은 기주 및 지리적 기원에 따라 유전적 다양성이 거의 없었다. CCYV는 박과작물 생산에 심각한 위협이 될 가능성이 있다. 수박, 잡초 등을 포함한 다양한 CCYV 분리주에 대한 병원성, 가루이 전염 등의 특성에 대한 추가적인 연구가 필요하다.

Keywords

Acknowledgement

This research was supported by Chungbuk National University Korea National University Development Project (2020).

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