The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Cucurbit Powdery Mildew: First Insights for the Identification of the Causal Agent and Screening for Resistance of Squash Genotypes (Cucurbita moschata (Duchesne ex Lam.) Duchesne ex Poir.) in Mendoza, Argentina

  • Received : 2022.01.04
  • Accepted : 2022.06.06
  • Published : 2022.08.01
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Abstract

The cucurbit powdery mildew (CPM) caused by different fungal species is a major concern for cucurbit crops around the world. In Argentina CPM constitutes the most common and damaging disease for cucurbits, especially for squash crops (Cucurbita moschata). The present study displays initial insights into the knowledge of the disease in western Argentina, including the determination of the prevalent species causing CPM, as well as the evaluation of the resistance of squash cultivars and breeding lines. Fungal colonies were isolated from samples collected in Mendoza province, Argentina. A field trial was also performed to assess the resistance of five squash accessions, including commercial cultivars and breeding lines. The severity of CPM was analyzed and epidemiological models were built based on empirical data. The morphological determinations and analysis with specific molecular markers confirmed Podosphaera xanthi as the prevalent causal agent of CPM in Mendoza. The results od the field trial showed differences in the resistance trait among the squash accessions. The advanced breeding line BL717/1 showed promising results as source of CPM resistance for the future development of open pollinated resistant cultivars, a crucial tool for an integrative control of the disease.

Keywords

Acknowledgement

The authors wish to thank Dr. Rients E. Niks for his critical reading of the manuscript and his useful annotations; to Eng. M. Rocio Rizzo for her assistance during the sampling; to Mr. Segundo Ramirez and the field crew of EEA La Consulta INTA for their help in field work. This study was funded by INTA Research Projects 2019-PE-E6-I508-001 Mejoramiento genetico de especies horticolas de uso intensivo and 2019-PD-E4-I090-001 Analisis de patosistemas en los principales cultivos agricolas y caracterizacion de sus componentes.

References

  1. Chen, R.-S., Chu, C., Cheng, C.-W., Chen, W.-Y. and Tsay, J.-G. 2008. Differentiation of two powdery mildews of sunflower (Helianthus annuus) by a PCR-mediated method based on ITS sequences. Eur. J. Plant Pathol. 121:1-8. https://doi.org/10.1007/s10658-007-9234-5
  2. Delhey, R., Braun, U. and Kiehr, M. 2003. Some new records of powdery mildew fungi from Argentina (2). Schlechtendalia 10:79-90.
  3. Della Gaspera, P. 2013. Manual del cultivo del zapallo Anquito (Cucurbita moschata Duch.). National Institute of Agricultural Technology, Mendoza, Argentina.
  4. Di-Rienzo, J. A., Casanoves, F., Balzarini, M. G., Gonzalez, L., Tablada, M. and Robledo, C. W. 2020. InfoStat. URL http://www.infostat.com.ar/ [4 January 2022].
  5. Kiehr, M. and Delhey, R. 2013. Enfermedades de Zapallo (Cucurbita spp.). In: Manual del cultivo del zapallo Anquito (Cucurbita moschata Duch.), ed. by P. Della Gaspera, pp. 189-244. National Institute of Agricultural Technology, Mendoza, Argentina.
  6. Kousik, C. S., Levi, A., Ling, K.-S. and Wechter, W. P. 2008. Potential sources of resistance to cucurbit powdery mildew in U.S. Plant Introductions of Bottle Gourd. HortScience 43:1359-1364. https://doi.org/10.21273/hortsci.43.5.1359
  7. Kristkova, E., Lebeda, A. and Sedlakova, B. 2009. Species spectra, distribution and host range of cucurbit powdery mildews in the Czech Republic, and in some other European and Middle Eastern countries. Phytoparasitica 37:337-350. https://doi.org/10.1007/s12600-009-0045-4
  8. Lebeda, A., Kristkova, E., Sedlakova, B., McCreight, J. D. and Coffey, M. D. 2016. Cucurbit powdery mildews: methodology for objective determination and denomination of races. Eur. J. Plant Pathol. 144:399-410. https://doi.org/10.1007/s10658-015-0776-7
  9. Lebeda, A. and Sedlakova, B. 2010. Screening for resistance to cucurbit powdery mildews (Golovinomyces cichoracearum, Podosphaera xanthii). In: Mass screening techniques for selecting crops resistant to disease, eds. by M. M. Spencer and A. Lebeda, pp. 295-307. International Atomic Energy Agency (IAEA), Vienna, Austria.
  10. Madden, L. V., Hughes, G. and van den Bosch, F. 2007a. The study of plant disease epidemics. American Phytopathological Society Press, St. Paul, MN, USA. 421 pp.
  11. Madden, L. V., Turechek, W. W. and Nita, M. 2007b. Evaluation of generalized linear mixed models for analyzing disease incidence data obtained in designed experiments. Plant Dis. 86:316-325. https://doi.org/10.1094/pdis.2002.86.3.316
  12. Mandal, M. K., Suren, H. and Kousik, C. 2020. Elucidation of resistance signaling and identification of powdery mildew resistant mapping loci (ClaPMR2) during watermelon-Podosphaera xanthii interaction using RNA-Seq and wholegenome resequencing approach. Sci. Rep. 10:14038. https://doi.org/10.1038/s41598-020-70932-z
  13. Mazzanti de Castanon, M. A., Alvarez, R. E. and Cabrera de Alvarez, M. G. 1987. A contribution to the aethiologic knowledge of powdery mildew on cucurbits cultivated in northeastern Argentine. Fitopatologia 22:21-29.
  14. Morishita, M., Sugiyama, K., Saito, T. and Sakata, Y. 2003. Powdery mildew resistance in cucumber. Jpn. Agric. Res. Q. 37:7-14. https://doi.org/10.6090/jarq.37.7
  15. Niks, R. E., Parlevliet, J. E., Lindhout, P. and Bai, Y. 2011. Breeding crops with resistance to diseases and pests. Wageningen Academic Publishers, Wageningen, The Netherlands. 200 pp.
  16. Perez-Garcia, A., Romero, D., Fernadez-Ortuno, D., Lopez-Ruiz, F., De Vicente, A. and Tores, J. A. 2009. The powdery mildew fungus Podosphaera fusca (synonym Podosphaera xanthii), a constant threat to cucurbits. Mol. Plant Pathol. 10:153-160. https://doi.org/10.1111/j.1364-3703.2008.00527.x
  17. Robinson, R. W. and Decker-Walters, D. S. 1997. Cucurbits: crop production science in horticulture. CAB International, Cambridge, UK. 240 pp.
  18. Silveira Maia, G. 2012. Isolation, identification and characterization of cucurbit powdery mildew in North Central Florida. Ph.D. thesis. University of Florida, Gainesville, FL, USA.
  19. Sistema Nacional Argentino de Vigilancia y Monitoreo de Plagas. 2021. Golovinomyces cichoracearum. URL https://www.sinavimo.gov.ar/plaga/golovinomyces-cichoracearum [3 January 2022].
  20. U.S. Geological Survey. 2022. URL https://www.usgs.gov/ [3 January 2022].