• Title/Summary/Keyword: cucumber plants

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Characterization and Sequence Analysis of a Lily Isolate of Cucumber mosaic virus from Lithium tsingtauense

  • Ryu, Ki-Hyun;Park, Hye-Won;Park, Jang-Kyung
    • The Plant Pathology Journal
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    • v.18 no.2
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    • pp.85-92
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    • 2002
  • A new isolate of Cucumber mosaic virus (CMV), identified as Li-CMV was isolated from a diseased Korean native lily (Lithium tsingtauense Gilg). Biological and serological properties of Li-CMV were characterized, and reverse transcription-polymerase chain reaction (RT-PCR) analysis, restriction enzyme profiling of RT-PCR products, and nucleotide sequence analysis of RNA3 of the virus were performed in this study. Remarkable differences in symptoms between Li-CMV and ordinary CMV strains were found in tobacco plants and Datura stramonium. Li-CMV-infected tobacco plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic ringspots on uninoculated upper leaves, and the symptom expression was delayed or faint whereas, ordinary CMV strains induced green mosaic symptoms on the plant. Systemic infections were observed on Nicotiana benthamiana with severe mosaic symptom. Restriction mapping analysis of RT-PCR products using MspI showed that Li-CMV belonged to CMV subgroup I. A full-length CDNA copy of RNA3 for the virus was amplified by RT-PCR, cloned, and its complete nucleotide sequence was determined. The RNA3 of Li-CMV was 2, 232 nucleotides long, and consisted of two open reading frames of 843 and 657 bases encoding 3a protein (movement protein) and coat protein, respectively. Results of this study indicate that Li-CMV is a novel strain and belongs to subgroup I of CMV in the genus Cucumovirus.

Biocontrol Potential of Fungal Endophytes against Fusarium oxysporum f. sp. cucumerinum Causing Wilt in Cucumber

  • Abro, Manzoor Ali;Sun, Xiang;Li, Xingchun;Jatoi, Ghulam Hussain;Guo, Liang-Dong
    • The Plant Pathology Journal
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    • v.35 no.6
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    • pp.598-608
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    • 2019
  • Endophytic fungi have received much attention as plant growth promoters as well as biological control agents against many plant pathogens. In this study, 30 endophytic fungal species, isolated from various plants in China, were evaluated using in vitro dual culture assay against Fusarium oxysporum f. sp. cucumerinum, causing wilt in cucumber. The results of the present study clearly showed that all the 30 endophytic fungal isolates were highly capable of inhibiting the mycelial colony growth of Fusarium oxysporum f. sp. cucumerinum with inhibition % over 66% as compared to control treatments. Among all of them, 5 isolates were highly effective such as, Penicillium sp., Guignardia mangiferae, Hypocrea sp., Neurospora sp., Eupenicillium javanicum, and Lasiodiplodia theobromae, respectively. The Penicillium sp. and Hypocrea sp. were highly effective as compared to other isolates. From in vitro results 10 best isolates were selected for greenhouse studies. The results of the greenhouse studies showed that among all of them 3 endophytic fungal isolates successfully suppressed wilt severity when co-inoculation with pathogen Fusarium. oxysporum f. sp. cucumerinum. The endophytic fungi also enhanced plant growth parameters of the host plants, the antagonistic fungal isolates increased over all plant height, aerial fresh, and dry weight as compared to control.

Rapid Detection and Identification of Cucumber Mosaic Virus by Reverse Transcription and Polymerase Chain Reaction (RT-PCR) and Restriction Analysis (역전사 중합효소련쇄반응(RT-PCR)과 제한효소 분석을 이용한 오이 모자이크 바이러스의 신속한 검정과 동정)

  • Park, Won Mok
    • Journal of Plant Biology
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    • v.38 no.3
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    • pp.267-274
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    • 1995
  • Based upon the nucleotide sequence of As strain of cucumber mosaic virus (CMV-As0 RNA4, coat protein (CP) gene was selected for the design of oligonucleotide primers of polymerase chain reaction (PCR) for detection and identification of the virus. Reverse transcription and polymerase chain reaction (RT-PCR) was performed with a set of 18-mer CMV CP-specific primers to amplify a 671 bp fragment from crude nucleic acid extracts of virus-infected leaf tissues as well as purified viral RNAs. The minimum concentrations of template viral RNA and crude nucleic acids from infected tobacco tissue required to detect the virus were 1.0 fg and 1:65,536 (w/v), respectively. No PCR product was obtained when potato virus Y-VN RNA or extracts of healthy plants were used as templates in RT-PCR using the same primers. The RT-PCR detected CMV-Y strain as well as CMV-As strain. Restriction analysis of the two individual PCR amplified DNA fragments from CMV-As and CMV-Y strains showed distinct polymorphic patterns. PCR product from CMV-As has a single recognition site for EcoRI and EcoRV, respectively, and the product from CMV-Y has no site for EcoRI or EcoRV but only one site for HindIII. The RT-PCR was able to detect the virus in the tissues of infected pepper, tomato and Chinese cabbage plants.

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Occurence of Viruses in Lilies (Lilium spp.) in Highland Areas and Their Detection by One-step RT-PCR (고랭지 나리의 바이러스 발생과 RT-PCR에 의한 검정)

  • 김수정;함영일;신관용;류승열;유동림;정효원;최장경
    • Research in Plant Disease
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    • v.7 no.2
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    • pp.80-85
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    • 2001
  • This study was carried out to examine tne incidences of virus diseases in lily plants cultivated in highland areas, and to develop an effective detection method. Viral symptoms on lilies in the highland areas were differentiated into mosaic, crinkle, mottle, stripe and line pattern. The distribution of symptoms on infected plants was 43.8% of mosaic, 29.2% of crinkle, and 10.9% of mottle symptoms. Six viruses such as Lily symptomless vires(LSV), Cucumber mosaic virus (CMV), Lily mottle virus (LMoV), Lily virus X (LVX, Potexvirus), Tabacco mosaic virus (TMV,Tobamovirus), and Tabacco rattle virus (TRV,Tobravirus) were detected from the infected lilies. Infection rate of Lilium oriental (cvs. Casablanca and Marcopolo) was 2~4 times higher than that of L. asiatic (cvs. Solemio and Prato). Virus detection on lilies by one-step RT-PCR (by using reverse transcription and polymerase chain reaction simultaneously) was more rapid rapid and reliable than by the conventional RT-PCR method.

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Development of a Robotic Transplanter Using Machine Vision for Bedding Plants (기계시각을 이용한 육묘용 로봇 이식기의 개발)

  • 류관희;김기영;이희환;한재성;황호준
    • Journal of Bio-Environment Control
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    • v.6 no.1
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    • pp.55-65
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    • 1997
  • This study was conducted to develop a robotic transplanter for bedding plants. The robotic transplanter consisted of machine vision system, manipulator attached with the specially designed gripper, and plug tray transfer system. Results of this study were as follows. 1. A machine vision system for a robotic transplanter was developed. The success rates of detecting empty cells and bad seedlings in 72-cell and 128-cell plug-trays for cucumber seedlings were 98.8% and 94.9% respectively. The success rates of identifying leaf orientation for 72- cell and 128-cell plug-trays were 93.5% and 91.0%, respectively. 2. A cartesian coordinate manipulator for a robotic transplanter with 3 degrees of freedom was constructed. The accuracy of position control was $\pm$ 1mm. 3. The robotic transplanter was tested with a shovel-type finger. Without considering leaf orientation, the success rates of transplanting healthy cucumber seedlings for 72-cell and 128-cell plug-trays were 95.5% and 94.5%, respectively. Considering leaf orientation, the success rates of transplanting healthy cucumber seedling in 72-cell and 128-cell plug-trays were 96.0% and 95.0%, respectively.

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Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum (오이 덩굴쪼김병에 대한 효율적인 저항성 검정 방법)

  • Lee, Ji Hyun;Kim, Jin-Cheol;Jang, Kyoung Soo;Choi, Yong Ho;Choi, Gyung Ja
    • Research in Plant Disease
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    • v.20 no.4
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    • pp.245-252
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    • 2014
  • The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or $30^{\circ}C$ than at $20^{\circ}C$. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of $1.0{\times}10^6-1.0{\times}10^7$ conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at $25^{\circ}C$ for 3 weeks to develop Fusarium wilt.

Effects of Deep Sea Water Treatment on the Inhibition of Over-Growth of Plug Seedlings (해양심층수 처리가 육묘 시 도장억제에 미치는 영향)

  • Kang Won-He;Hong Sung-Yu
    • Korean Journal of Organic Agriculture
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    • v.14 no.1
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    • pp.85-95
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    • 2006
  • The investigation of the effect of the deep sea water in different concentration (0%, 10%, 20%, 30%, 40%) on the inhibition of growth, fresh weight, dry weight of the tomato, hot pepper, cucumber plug seedlings were conducted. As the concentration of the deep sea water was higher, the greater inhibition was noticed. The height were decreased at the rate of 8%, 25%, 32% in tomato, 9%, 26%, 27% in hot pepper, and 21%, 50%, 58% in cucumber plug seedlings as compare to local check. The fresh weights were decreased highly, as the concentration of the deep sea water increased. Tomato seedlings showed the decrease rate of 12%, 23%, 33% at above the ground part of plants, and 2%, 30%, 52% at under the ground parts. Likewise, Hot pepper seedlings showed the decrease rate of 1.1%, 5.7%, 15.4% at above the ground part of plants, and 22.1%, 25%, 47.1%, respectively at under the ground parts. The fresh weight of cucumber was decreased at the rate of 8.1%, 36%, 51% in the above the ground, and 6.2%, 11%, 65% at the under the ground parts. In tomato, hot pepper, and cucumber seedlings, the dry weight was decreased as the concentration of deep sea water was increased. Thirty percent of deep sea water treatment reduced the dry weight of the seedlings as half of the control seedlings. One of the important factors of the seedling quality is compactness. Compactness was highest at 20% deep sea water in all the three crop seedlings.

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Supplemental Lighting by HPS and PLS Lamps Affects Growth and Yield of Cucumber during Low Radiation Period (약광기 HPS와 PLS lamp를 이용한 오이의 보광재배효과)

  • Kwon, Joon-Kook;Yu, In-Ho;Park, Kyoung-Sub;Lee, Jae-Han;Kim, Jin-Hyun;Lee, Jung-Sup;Lee, Dong-Soo
    • Journal of Bio-Environment Control
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    • v.27 no.4
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    • pp.400-406
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    • 2018
  • In this experiment the effect of supplemental lighting on the growth and yield of cucumber (Cucumis sativus L. 'Fresh') plants during low radiation period of winter season were investigated in glasshouses using common high-pressure sodium (HPS) lamps and newly developed plasma lighting system (PLS) lamps. Plants grown without supplemental lighting were considered as a control. Supplemental lighting was provided from November 20th, 2015 to March 15th, 2016 to ensure 14-hour photoperiod (natural+supplemental light), also lamps were operated automatically when the outside sun radiation levels were less than $100W{\cdot}m^{-2}$. Spectral analysis showed that HPS lamp had a discrete spectrum, lacked of the radiation in the 400-550 nm wave band (blue-green light), but had a high output in the orange-red region (550-650 nm). A higher red light output resulted in an increased red to far-red (R/FR) ratio in HPS lamp. PLS had a continuous spectrum and had a peak radiation in green region (490-550 nm). HPS has 12.6% lower output in photosynthetically active radiation (PAR) but 12.6% higher output in near infra-red (NIR) spectral regions compared to PLS. Both HPS and PLS lamps emitted very low levels of ultra-violet radiation (300-400 nm). Supplemental lighting both from HPS and PLS lamps increased plant height, leaf number, internode number and dry weight of cucumber plants compared to control. Photosynthetic activity of cucumber plants grown under two supplemental lighting systems was comparable. Number of fruits per cucumber plant (fruit weight per plant) in control, PLS, and HPS plots were 21.2 (2.9 kg), 38.7 (5.5 kg), and 40.4 (5.6 kg), respectively, thereby increasing yield by 1.8-1.9 times in comparison with control. An analysis of the economic feasibility of supplemental lighting in cucumber cultivation showed that considering lamp installation and electricity costs the income from supplemental lighting increased by 37% and 62% for PLS and HPS lamps, respectively.

Analysis of Water Stress of Greenhouse Crops Using Infrared Thermography (열영상 정보를 이용한 온실 재배 작물의 수분 스트레스 분석)

  • 김기영;류관희;채희연
    • Journal of Biosystems Engineering
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    • v.24 no.5
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    • pp.439-444
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    • 1999
  • Automated greenhouse production systems often require crop growth monitoring involving accurate quantification of plant physiological properties. Conventional methods are usually burdensome, inaccurate, and harmful to crops. A thermal image analysis system can accomplish rapid and accurate measurements of physiological-property changes of stressed crops. In this research a thermal imaging system was used to measure the leaf-temperature changes of several crops according to water deficit. Thermal images were obtained from lettuce, cucumber, pepper, and chinese cabbage plants. Results showed that there were significant differences in the temperature of stressed plants and non-stressed plants. The temperature differences between these two group of plants were 0.7 to 3$^{\circ}C$ according to species.

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A Study on the Vegetables Mentioned in the Bible (성서에 언급된 채소류에 관한 연구)

  • Woo, Ja-Won
    • Journal of the Korean Society of Food Culture
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    • v.22 no.1
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    • pp.1-9
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    • 2007
  • This paper is intended to study what kinds of the vegetables are mentioned in the Bible and how they were used in those days. While one hundred and twenty-eight different plants are mentioned in the Bible, there are today 2,384 plant species in modem Israel, most of which have been introduced in recent centuries. These plants obviously did not exist there in biblical times and were only recently introduced from Australia and South America, respectively. This article will study only the vegetables mentioned in the Bible and known to have existed in the old and new testament times. Since the first book devoted exclusively to biblical botany was that of Levinus Lemmens in 1566, the modem systematic study of biblical plants, began with F. Hasselquist, a student of Linnaeus, the founder of modem botany. In 1928, Immanuel Loew approached the subject differently, reviewing all known data pertaining to biblical plants. His work not only discussed biblical plants, but also plants in later Jewish literature, particularly the Talmud. The British scholar G. E. Post provided a broad field study of modem plants in Palestine, Syria, Lebanon, and Jordan. More recent major treatments of the subject include those of A. and H. Moldenke (1952), M. Zohary (1982), N. Hareuveni (1984), and Y. J. Choi(1996). Today, articles on specific biblical plants listed in the Bible can be found in any number of encyclopedias. This study attempts to provide a synthesis of the work of a number of scholars who studied the vegetables and plants mentioned in the Bible. As a preliminary study on the culture of food in the biblical period, this study has focused on the identity and features of the vegetables of the Bible. In only a limited number of instances, because of the paucity of the informations and the broad and generic descriptions of the plants, we can't be certain about the identification of the vegetables named in the Bible. In many instances the traditions established by the Greek, Aramaic, and English translations are helpful, although sometimes they are misleading. This paper subdivides the vegetables into broad areas, the general vegetables and the flavoring herbs. Vegetables formed very important part of the diet in the biblical times. Two main types were used: those whose nutritious seeds could be easily stored and those which were eaten freshly gathered from gardens. Pulse seeds provided a useful source of vegetable protein, while fresh green vegetables were vitamin rich. Pulses could be eaten boiled, or their dried seeds could be ground up into flour and then made into nutritious soups. Fresh vegetables were eaten either raw or lightly cooked, usually by boiling in water. The general vegetables in the Bible are herbs(garden rocket), cucumber(snake cucumber), watermelon, leeks, chicory, and onions. Also the flavoring herbs in the Bible are rue, dill, cummin, black cummin, frankincense, cinnamon, cassia, myrrh, black mustard, coriander, mint, saffron, ginger grass, syrian hyssop, aloes(eagle wood), manna which have the flavor, aroma, and medical values.