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http://dx.doi.org/10.5423/RPD.2014.20.4.245

Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum  

Lee, Ji Hyun (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Kim, Jin-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University)
Jang, Kyoung Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Choi, Yong Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Choi, Gyung Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
Publication Information
Research in Plant Disease / v.20, no.4, 2014 , pp. 245-252 More about this Journal
Abstract
The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or $30^{\circ}C$ than at $20^{\circ}C$. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of $1.0{\times}10^6-1.0{\times}10^7$ conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at $25^{\circ}C$ for 3 weeks to develop Fusarium wilt.
Keywords
Breeding; Disease resistance; Fusarium wilt; Resistant screening;
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