Browse > Article
http://dx.doi.org/10.5423/PPJ.OA.05.2019.0129

Biocontrol Potential of Fungal Endophytes against Fusarium oxysporum f. sp. cucumerinum Causing Wilt in Cucumber  

Abro, Manzoor Ali (Department of Plant Pathology, Faculty of Crop Protection, Sindh Agriculture University Tandojam)
Sun, Xiang (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences No. 1)
Li, Xingchun (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences No. 1)
Jatoi, Ghulam Hussain (Department of Plant Pathology, Faculty of Crop Protection, Sindh Agriculture University Tandojam)
Guo, Liang-Dong (State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences No. 1)
Publication Information
The Plant Pathology Journal / v.35, no.6, 2019 , pp. 598-608 More about this Journal
Abstract
Endophytic fungi have received much attention as plant growth promoters as well as biological control agents against many plant pathogens. In this study, 30 endophytic fungal species, isolated from various plants in China, were evaluated using in vitro dual culture assay against Fusarium oxysporum f. sp. cucumerinum, causing wilt in cucumber. The results of the present study clearly showed that all the 30 endophytic fungal isolates were highly capable of inhibiting the mycelial colony growth of Fusarium oxysporum f. sp. cucumerinum with inhibition % over 66% as compared to control treatments. Among all of them, 5 isolates were highly effective such as, Penicillium sp., Guignardia mangiferae, Hypocrea sp., Neurospora sp., Eupenicillium javanicum, and Lasiodiplodia theobromae, respectively. The Penicillium sp. and Hypocrea sp. were highly effective as compared to other isolates. From in vitro results 10 best isolates were selected for greenhouse studies. The results of the greenhouse studies showed that among all of them 3 endophytic fungal isolates successfully suppressed wilt severity when co-inoculation with pathogen Fusarium. oxysporum f. sp. cucumerinum. The endophytic fungi also enhanced plant growth parameters of the host plants, the antagonistic fungal isolates increased over all plant height, aerial fresh, and dry weight as compared to control.
Keywords
biocontrol; cucumber; Fusarium wilt; fungal endophytes;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Wiyakrutta, S., Sriubolmas, N., Panphut, W., Thongon, N., Danwisetkanjana, K., Ruangrungsi, N. and Meevootisom, V. 2004. Endophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plants. World J. Microbiol. Biotechnol. 20:265-272.   DOI
2 Worapong, J. and Strobel, G. A. 2009. Biocontrol of a root rot of kale by Muscodor albus strain MFC2. BioControl 54:301-306.   DOI
3 Wu, L., Shang, H., Wang, Q., Gu, H., Liu, G. and Yang, S. 2016. Isolation and characterization of antagonistic endophytes from Dendrobium candidum Wall ex Lindl., and the biofertilizing potential of a novel Pseudomonas saponiphila strain. Appl. Soil Ecol. 105:101-108.   DOI
4 Gava, C. A. T. and Pinto, J. M. 2016. Biocontrol of melon wilt caused by Fusarium oxysporum Schlect f. sp melonis using seed treatment with Trichoderma spp. and liquid compost. Biol. Control 97:13-20.   DOI
5 Zhang, Q., Zhang, J., Yang, L., Zhang, L., Jiang, D., Chen, W. and Li, G. 2014. Diversity and biocontrol potential of endophytic fungi in Brassica napus. Biol. Control 72:98-108.   DOI
6 Xiang, L., Gong, S., Yang, L., Hao, J., Xue, M., Zeng, F., Zhang, X., Shi, W., Wang, H. and Yu, D. 2016. Biocontrol potential of endophytic fungi in medicinal plants from Wuhan Botanical Garden in China. Biol. Control 94:47-55.   DOI
7 Youssef, S. A., Tartoura, K. A. and Abdelraouf, G. A. 2016. Evaluation of Trichoderma harzianum and Serratia proteamaculans effect on disease suppression, stimulation of ROSscavenging enzymes and improving tomato growth infected by Rhizoctonia solani. Biol. Control 100:79-86.   DOI
8 Zeilinger, S., Gupta, V. K., Dahms, T. E. S., Silva, R. N., Singh, H. B., Upadhyay, R. S., Gomes, E. V., Tsui, C. K.-M. and Nayak, S. C. 2016. Friends or foes? Emerging insights from fungal interactions with plants. FEMS Microbiol. Rev. 40:182-207.   DOI
9 Hanada, R. E., Pomella, A. W. V., Costa, H. S., Bezerra, J. L., Loguercio, L. L. and Pereira, J. O. 2010. Endophytic fungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuacu) trees and their potential for growth promotion and biocontrol of black-pod disease. Fungal Biol. 114:901-910.   DOI
10 Guo, L. D., Hyde, K. D. and Liew, E. C. Y. 2000. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol. 147:617-630.   DOI
11 Hong, P.-X., Qiu, S.-X., Chen, H., Zhao, X.-D. and Hu, F.-P. 2007. Isolation and screening of endophytic antagonistic bacteria from four species of Solanaceae. J. Fujian Agric. For. Univ. 36:347-351 (in Chinese).   DOI
12 Kaul, S., Gupta, S., Ahmed, M. and Dhar, M. K. 2012. Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochem. Rev. 11:487-505.   DOI
13 Kim, H.-Y., Choi, G. J., Lee, H. B., Lee, S. W., Lim, H. K., Jang, K. S., Son, S. W., Lee, S. O., Cho, K. Y., Sung, N. D. and Kim, J.-C. 2007. Some fungal endophytes from vegetable crops and their anti-oomycete activities against tomato late blight. Lett. Appl. Microbiol. 44:332-337.   DOI
14 Kim, J.-C., Choi, G. J., Park, J.-H., Kim, H. T. and Cho, K. Y. 2001. Activity against plant pathogenic fungi of phomalactone isolated from Nigrospora sphaerica. Pest Manag. Sci. 57:554-559.   DOI
15 Lim, G. T. T., Wang, G.-P., Hemming, M. N., Basuki, S., Mc-Grath, D. J., Carroll, B. J. and Jones, D. A. 2006. Mapping the I-3 gene for resistance to Fusarium wilt in tomato: application of an I-3 marker in tomato improvement and progress towards the cloning of I-3. Australas. Plant Pathol. 35:671-680.   DOI
16 McGovern, R. J. 2015. Management of tomato diseases caused by Fusarium oxysporum. Crop Prot. 73:78-92.   DOI
17 Lu, D., Ma, Z., Xu, X. and Yu, X. 2016. Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum. J. Basic Microbiol. 56:929-933.   DOI
18 Lugtenberg, B. J., Caradus, J. R. and Johnson, L. J. 2016. Fungal endophytes for sustainable crop production. FEMS Microbiol. Ecol. 92:fiw194.   DOI
19 Martinez, R., Aguilar, M. I., Guirado, M. L., Alvarez, A. and Gomez, J. 2003. First report of fusarium wilt of cucumber caused by Fusarium oxysporum in Spain. Plant Pathol. 52:410.   DOI
20 Medina-Cordova, N., Lopez-Aguilar, R., Ascencio, F., Castellanos, T., Campa-Cordova, A. I. and Angulo, C. 2016. Biocontrol activity of the marine yeast Debaryomyces hansenii against phytopathogenic fungi and its ability to inhibit mycotoxins production in maize grain (Zea mays L.). Biol. Control 97:70-79.   DOI
21 Mei, C. and Flinn, B. S. 2010. The use of beneficial microbial endophytes for plant biomass and stress tolerance improvement. Recent Pat. Biotechnol. 4:81-95.   DOI
22 Mejia, L. C., Rojas, E. I., Maynard, Z., van Bael, S., Arnold, A. E., Hebbar, P., Samuels, G. J., Robbins, N. and Herre, E. A. 2008. Endophytic fungi as biocontrol agents of Theobroma cacao pathogens. Biol. Control 46:4-14.   DOI
23 Miles, L. A., Lopera, C. A., Gonzalez, S., de Garcia, M. C. C., Franco, A. E. and Restrepo, S. 2012. Exploring the biocontrol potential of fungal endophytes from an Andean Colombian Paramo ecosystem. BioControl 57:697-710.   DOI
24 Norman, C. 1988. EPA sets new policy on pesticide cancer risks. Science 242:366-367.   DOI
25 Posada, F. and Vega, F. E. 2006. Inoculation and colonization of coffee seedlings (Coffea arabica L.) with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales). Mycoscience 47:284-289.   DOI
26 Ownley, B. H., Gwinn, K. D. and Vega, F. E. 2010. Endophytic fungal entomopathogens with activity against plant pathogens: ecology and evolution. BioControl 55:113-128.   DOI
27 Patel, H. A., Patel, R. K., Khristi, S. M., Parikh, K. and Rajendran, G. 2012. Isolation and characterization of bacterial endophytes from Lycopersicon esculentum plant and their plant growth promoting characteristics. Nepal J. Biotechnol. 2:37-52.
28 Paulitz, T. C., Park, C. S. and Baker, R. 1987. Biological control of Fusarium wilt of cucumber with nonpathogenic isolates of Fusarium oxysporum. Can. J. Microbiol. 33:349-353.   DOI
29 Prabhukarthikeyan, R., Saravanakumar, D. and Raguchander, T. 2014. Combination of endophytic Bacillus and Beauveria for the management of Fusarium wilt and fruit borer in tomato. Pest Manag. Sci. 70:1742-1750.   DOI
30 Premalatha, K. and Kalra, A. 2013. Molecular phylogenetic identification of endophytic fungi isolated from resinous and healthy wood of Aquilaria malaccensis, a red listed and highly exploited medicinal tree. Fungal Ecol. 6:205-211.   DOI
31 Radic, N. and Strukelj, B. 2012. Endophytic fungi: the treasure chest of antibacterial substances. Phytomedicine 19:1270-1284.   DOI
32 Singh, P. P., Shin, Y. C., Park, C. S. and Chung, Y. R. 1999. Biological control of Fusarium wilt of cucumber by chitinolytic bacteria. Phytopathology 89:92-99.   DOI
33 Rai, M., Rathod, D., Agarkar, G., Dar, M., Brestic, M., Pastore, G. M. and Marostica, M. R. Jr. 2014. Fungal growth promotor endophytes: a pragmatic approach towards sustainable food and agriculture. Symbiosis 62:63-79.   DOI
34 Bae, S.-J., Mohanta, T. K., Chung, J. Y., Ryu, M., Park, G., Shim, S., Hong, S.-B., Seo, H., Bae, D.-W., Bae, I., Kim, J.-J. and Bae, H. 2016. Trichoderma metabolites as biological control agents against Phytophthora pathogens. Biol. Control 92:128-138.   DOI
35 Barretti, P. B., Romeiro, R. S., Mizubuti, E. S. G. and de Souza, J. T. 2009. Screening of endophytic bacteria isolated from tomato plants as potencial biocontrol agents and growth promotion. Cienc. Agrotec. 33 Suppl:2038-2044 (in Portuguese).   DOI
36 Rania, A. B. A., Jabnoun-Khiareddine, H., Nefzi, A., Mokni-Tlili, S. and Daami-Remadi, M. 2016. Endophytic bacteria from Datura metel for plant growth promotion and bioprotection against Fusarium wilt in tomato. Biocontrol Sci. Technol. 26:1139-1165.   DOI
37 Raza, W., Ling, N., Zhang, R., Huang, Q., Xu, Y. and Shen, Q. 2017. Success evaluation of the biological control of Fusarium wilts of cucumber, banana, and tomato since 2000 and future research strategies. Crit. Rev. Biotechnol. 37:202-212.   DOI
38 Reis, A., Costa, H., Boiteux, L. S. and Lopes, C. A. 2005. First report of Fusarium oxysporum f. sp. lycopersici Race 3 on tomato in Brazil. Fitopatol. Bras. 30:426-428.   DOI
39 Saravanakumar, K., Yu, C., Dou, K., Wang, M., Li, Y. and Chen, J. 2016. Synergistic effect of Trichoderma-derived antifungal metabolites and cell wall degrading enzymes on enhanced biocontrol of Fusarium oxysporum f. sp. cucumerinum. Biol. Control 94:37-46.   DOI
40 Strobel, G. A. 2003. Endophytes as sources of bioactive products. Microbes Infect. 5:535-544.   DOI
41 Eksteen, D., Pretorius, J. C., Nieuwoudt, T. D. and Zietsman, P. C. 2001. Mycelial growth inhibition of plant pathogenic fungi by extracts of South African plant species. Ann. Appl. Biol. 139:243-249.   DOI
42 Costa, F. G., Zucchi, T. D. and de Melo, I. S. 2013. Biological control of phytopathogenic fungi by endophytic actinomycetes isolated from maize (Zea mays L.). Braz. Arch. Biol. Technol. 56:948-955.   DOI
43 Crozier, J., Arroyo, C., Morales, H., Melnick, R. L., Strem, M. D., Vinyard, B. T., Collins, R., Holmes, K. A. and Bailey, B. A. 2015. The influence of formulation on Trichoderma biological activity and frosty pod rot management in Theobroma cacao. Plant Pathol. 64:1385-1395.   DOI
44 Debbab, A., Aly, A. H. and Proksch, P. 2013. Mangrove derived fungal endophytes: a chemical and biological perception. Fungal Divers. 61:1-27.   DOI
45 El Komy, M. H., Saleh, A. A., Ibrahim, Y. E., Hamad, Y. K. and Molan, Y. Y. 2016. Trichoderma asperellum strains confer tomato protection and induce its defense-related genes against the Fusarium wilt pathogen. Trop. Plant Pathol. 41:277-287.   DOI
46 Fan, A. M. and Jackson, R. J. 1989. Pesticides and food safety. Regul. Toxicol. Pharmacol. 9:158-174.   DOI
47 Alabouvette, C., Lemanceau, P. and Steinberg, C. 1993. Recent advances in the biological-control of Fusarium wilts. Pestic. Sci. 37:365-373.   DOI
48 Vethavalli, S. and Sudha, S. S. 2012. In vitro and in silico studies on biocontrol agent of bacterial strains against Fusarium oxysporum f. sp. lycopersici. Res. Biotechnol. 3:22-31.
49 Vos, C. M., Yang, Y., De Coninck, B. and Cammue, B. P. A. 2014. Fungal (-like) biocontrol organisms in tomato disease control. Biol. Control 74:65-81.   DOI
50 Wang, S., Liang, Y., Shen, T., Yang, H. and Shen, B. 2016. Biological characteristics of Streptomyces albospinus CT205 and its biocontrol potential against cucumber Fusarium wilt. Biocontrol Sci. Technol. 26:951-963.   DOI
51 Anith, K. N., Sreekumar, A. and Sreekumar, J. 2015. The growth of tomato seedlings inoculated with co-cultivated Piriformospora indica and Bacillus pumilus. Symbiosis 65:9-16.   DOI
52 Arnold, A. E., Maynard, Z. and Gilbert, G. S. 2001. Fungal endophytes in dicotyledonous neotropical trees: patterns of abundance and diversity. Mycol. Res. 105:1502-1507.   DOI
53 Aydi Ben Abdallah, R., Mokni-Tlili, S., Nefzi, A., Jabnoun-Khiareddine, H. and Daami-Remadi, M. 2016. Biocontrol of Fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Nicotiana glauca organs. Biol. Control 97:80-88.   DOI
54 Backman, P. A. and Sikora, R. A. 2008. Endophytes: An emerging tool for biological control. Biol. Control 46:1-3.   DOI