Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Efficient Screening Method for Resistance of Cucumber Cultivars to Fusarium oxysporum f. sp. cucumerinum

오이 덩굴쪼김병에 대한 효율적인 저항성 검정 방법

  • Lee, Ji Hyun (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Kim, Jin-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Environmentally-Friendly Agriculture, Chonnam National University) ;
  • Jang, Kyoung Soo (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong Ho (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung Ja (Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology)
  • 이지현 (한국화학연구원 바이오화학연구센터) ;
  • 김진철 (전남대학교 응용생물공학부) ;
  • 장경수 (한국화학연구원 바이오화학연구센터) ;
  • 최용호 (한국화학연구원 바이오화학연구센터) ;
  • 최경자 (한국화학연구원 바이오화학연구센터)
  • Received : 2014.09.03
  • Accepted : 2014.10.31
  • Published : 2014.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The study was performed to establish an efficient screening method for resistant cucumber to Fusarium oxysporum f. sp. cucumerinum. The isolate KR5 was identified as F. oxysporum f. sp. cucumerinum based on molecular analyses of ITS and TEF genes and host-specificity test on cucurbits including melon, oriental melon, cucumber, and watermelon. Then four cucumber and two rootstock cultivars showing different resistance degrees to the Fusarium wilt pathogen KR5 were selected. And development of Fusarium wilt of the six cultivars according to several conditions, including incubation temperature after inoculation, inoculum concentration, root wounding, and growth stages of seedlings, was investigated. Disease severity of Fusarium wilt on the resistant cultivars was changed with incubation temperatures after inoculation. The resistant cultivars showed the higher resistance when inoculated plants were kept at 25 or $30^{\circ}C$ than at $20^{\circ}C$. Among four different growth stages of the seedlings, seven-day-old seedling represented the most difference of resistance and susceptibility to Fusarium wilt. From above results, we suggest that an efficient screening method for resistant cucumber to F. oxysporum f. sp. cucumerinum is to dip the non-cut roots of seven-day-old seedlings in spore suspension of $1.0{\times}10^6-1.0{\times}10^7$ conidia/ml and to transplant the seedling into a non-infected soil, and then to incubate the inoculated plants in a growth room at $25^{\circ}C$ for 3 weeks to develop Fusarium wilt.

본 실험은 Fusarium oxysporum f. sp. cucumerinum에 의해 발생하는 오이 덩굴쪼김병의 효율적인 저항성 검정 방법을 확립하기 위하여 수행되었다. ITS와 TEF 유전자 염기서열 분석과 멜론, 참외, 오이 및 수박을 포함한 박과 작물에 대한 기주 특이성 분석을 통해 KR5 균주를 F. oxysporum f. sp. cucumerinum으로 동정하였다. 그리고 덩굴쪼김병균 KR5에 저항성 정도가 다른 오이 네 품종과 오이용 대목 두 품종을 선발하여 유묘의 생육 시기, 뿌리 상처, 접종원 농도 및 접종 후 재배 온도에 따라 이들 여섯 품종의 덩굴쪼김병 발생을 조사하였다. 저항성 품종의 덩굴쪼김병 발병도는 접종 후 재배 온도에 따라 차이를 나타냈으며, 저항성 품종들은 접종 후에 $20^{\circ}C$보다 $25^{\circ}C$나 $30^{\circ}C$에서 재배하였을 때 높은 저항성을 보였다. 그리고 유묘의 생육 시기 중 파종 후 7일된 유묘에 덩굴쪼김병균을 접종하였을 때 가장 큰 저항성과 감수성 차이를 나타내었다. 이상의 결과로부터 오이 덩굴쪼김병에 대한 저항성을 효과적으로 검정하는 방법으로 파종하고 7일 동안 재배한 유묘의 뿌리로부터 흙을 제거하고, 이들을 $1.0{\times}10^6-1.0{\times}10^7$ conidia/ml 농도의 F. oxysporum f. sp. cucumerinum 포자현탁액에 침지하여 접종한 후에 건전한 원예용 상토에 이식하고 $25^{\circ}C$ 생육상에서 3주간 재배하는 방법을 제안한다.

Keywords

References

  1. Ahn, I. P., Chung, H. S. and Lee, Y. H. 1998. Vegetative compatibility groups and pathogenicity among isolates of Fusarium oxysporum f. sp. cucumerinum. Plant Dis. 82: 244-246. https://doi.org/10.1094/PDIS.1998.82.2.244
  2. Armstrong, G. M. and Armstrong, J. K. 1981. Formae speciales and races of Fusarium oxysporum causing wilt disease. In: Fusarium: Disease, Biology, and Taxonomy, ed. by P. E. Nelson, T. A. Toussoun and R. J. Cook, pp. 391-399. Pennsylvania State University Press, University Park.
  3. Armstrong, G. M., Armstrong, J. K. and Netzer, D. 1978. Pathogenic races of the cucumber-wilt Fusarium. Plant Dis. 62: 824-828.
  4. Both, C. 1971. The genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, England.
  5. Bouhot, D. 1981. Some aspects of the pathogenic potential in formae speciales and races of Fusarium oxysporum on Cucurbitaceae. In: Fusarium: Disease, Biology, and Taxonomy. ed. by P. E. Nelson, T. A. Toussoun and R. J. Cook, pp. 318-326. Pennsylvania State University Press, University Park.
  6. Chen, F., Wang, M., Zheng, Y., Luo, J. M., Yang, X. R. and Wang, X. L. 2010. Quantitative changes of plant defense enzymes and phytohormone in biocontrol of cucumber Fusarium wilt by Bacillus subtilis B579. World J. Microbiol. Biotechnol. 26: 675-684. https://doi.org/10.1007/s11274-009-0222-0
  7. Chun, J. 1995. Computer-assisted classification and identification of Actinomycetes. Ph. D. thesis, University of Newcastle, Newcastle upon Tyne, UK.
  8. Davis, D. 1966. Cross-infection in Fusarium wilt diseases. Phytopathology 56: 825-828.
  9. Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791. https://doi.org/10.2307/2408678
  10. Freeman, S. and Rodriquez, R. J. 1993. A rapid inoculation technique for assessing pathogenicity of Fusarium oxysporum f. sp. niveum and F. o. melonis on cucurbits. Plant Dis. 77: 1198-1201. https://doi.org/10.1094/PD-77-1198
  11. Freeman, S., Zveibil, A., Vintal, H. and Maymon, M. 2002. Isolation of nonpathogenic mutants of Fusarium oxysporum f. sp. melonis for biological control of Fusarium wilt in cucurbits. Phytopathology 92: 164-168. https://doi.org/10.1094/PHYTO.2002.92.2.164
  12. Gerlagh, M. and Blok, W. J. 1988. Fusarium oxysporum f. sp. cucurbitacearum n. f. embracing all formae speciales of F. oxysporum attacking cucurbitaceous crops. Neth. J. Plant Pathol. 94: 17-31. https://doi.org/10.1007/BF01999804
  13. Kanno, T., Han, X. and Tabei, Y. 1991. Genetic analysis of resistance in cucumber to Fusarium oxysporum f. sp. cucumerinum. Bull. Natl. Res. Inst. Veg. Ornament. Plants Tea 4: 27-30.
  14. Kawai, I., Suzuki, H. and Kawai, K. 1958. On the pathogenicity of the wilt Fusarium of the cucurbitaceous plants and their forms. Shizuoka Agric. Exp. Stn. Bull. 3: 49-68.
  15. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequence. J. Mol. Evol. 16: 111-120. https://doi.org/10.1007/BF01731581
  16. Latin, R. X. and Snell, S. J. 1986. Comparison of methods for inoculation of muskmelon with Fusarium oxysporum f. sp. melonis. Plant Dis. 70: 297-300. https://doi.org/10.1094/PD-70-297
  17. Leach, J. G. and Currence, T. M. 1938. Fusarium wilt of muskmelon in Minnesota. Minn. Agric. Exp. Stn. Tech. Bull. 129: 32.
  18. Lee, S. G. and Lee, W. H. 1994. Control of Fusarium wilt of watermelon with the root-stock grafting of Sicyos angulatus L. Korean J. Plant Pathol. 10: 240-244.
  19. Martyn, R. D. and McLaughlin, R. J. 1983a. Effects of inoculum concentration on the apparent resistance of watermelons to Fusarium oxysporum f. sp. niveum. Plant Dis. 67: 493-495. https://doi.org/10.1094/PD-67-493
  20. Martyn, R. D. and McLaughlin, R. J. 1983b. Susceptibility of summer squash to the watermelon wilt pathogen (Fusarium oxysporum f. sp. niveum). Plant Dis. 67: 263-266. https://doi.org/10.1094/PD-67-263
  21. Matuo, T. and Yamamoto, I. 1957. On Fusarium oxysporum f. sp. lagenariae n. f. causing wilt of Lagenaria vulgaris var. hispida. Trans. Mycol. Soc. Jpn. 2: 61-63.
  22. McMillan, R. T. 1986. Cross pathogenicity studies with isolates of Fusarium oxysporum from either cucumber or watermelon pathogenic to both crop species. Ann. Appl. Biol. 109: 101-105. https://doi.org/10.1111/j.1744-7348.1986.tb03188.x
  23. Netzer, D., Niego, S. and Galun, E. 1977. A dominant gene conferring resistance to Fusarium wilt in cucumber. Phytopathology 67: 525-527.
  24. O' Donnell, K., Cigelnik, E. and Casper, H. H. 1998. Molecular phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum. Fungal Genet. Biol. 23: 57-67. https://doi.org/10.1006/fgbi.1997.1018
  25. Owen, J. H. 1955. Fusarium wilt of cucumber. Phytopathology 45: 435-439.
  26. Owen, J. H. 1956. Cucumber wilt, caused by Fusarium oxysporum f. sp. cucumerinum. Phytopathology 46: 153-157.
  27. Sherf, A. F. and MacNab, A. A. 1985. Vegetable disease and their control. John Wiley and Sons, New York. 728 pp.
  28. Snyder, W. C. and Hansen, H. N. 1940. The species concept in Fusarium. Am. J. Bot. 27: 64-67. https://doi.org/10.2307/2436688
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24: 4876-4882.
  30. Toshimitsh, Y. and Noguchi, T. 1975. Inheritance of resistance to Fusarium wilt in cucumber. Proc. Mtg. Jpn. Hort. Society 150-151.
  31. Vakalounakis, D. J. 1993. Inheritance and genetic linkage of fusarium wilt (Fusarium oxysporum f. sp. cucumerinum race 1) and scab (Cladosporium cucumerinum) resistance genes in cucumber (Cucumis sativus). Ann. Appl. Biol. 123: 359-365. https://doi.org/10.1111/j.1744-7348.1993.tb04098.x
  32. Vakalounakis, D. J. 1995. Inheritance and linkage of resistance in cucumber line SMR-18 to races 1 and 2 of Fusarium oxysporum f. sp. cucumerinum. Plant Pathol. 44: 169-172.
  33. Vakalounakis, D. J. 1996. Allelism of the Fcu-1 and Foc genes conferring resistance to Fusarium wilt in cucumber. Eur. J. Plant Pathol. 102: 855-858. https://doi.org/10.1007/BF01877055
  34. Vakalounakis, D. J. and Smardas, K. 1995. Genetics of resistance to Fusarium oxysporum f. sp. cucumerinum races 1 and 2 in cucumber line Wisconsin-2757. Ann. Appl. Biol. 127: 457-461. https://doi.org/10.1111/j.1744-7348.1995.tb07604.x
  35. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, In: PCR protocols: A guide to methods and applications, ed. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego.
  36. Yang, S. S. and Kim, C. H. 1994. Studies on cross protection of Fusarium wilt of cucumber III. Selection of nonpathogenic isolates and their protective effects in the greenhouse. Korean J. Plant Pathol. 10: 29-33. (In Korean)
  37. Zhou, X. G., Everts, K. L. and Bruton, B. D. 2010. Race 3, a new and highly virulent race of Fusarium oxysporum f. sp. niveum causing Fusarium wilt in watermelon. Plant Dis. 94: 92-98. https://doi.org/10.1094/PDIS-94-1-0092