• Reproductive and Developmental Biology
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• 제32권1호
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• pp.15-20
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• 2008

Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.

• 한국정밀공학회지
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• 제19권9호
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• pp.172-178
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• 2002

One of the ever-increasing demands on the performances of heterodyne interferometers is to improve the measurement resolution, of which current state -of-the-art reaches the region of sub-nanometers. So far, the demand has been met by increasing the clock speed that drives the electronics involved fur the phase measurement of the Doppler shift, but its further advance is being hampered by the technological limit of modem electronics. To cope with the problem, in this investigation, we propose a new scheme of phase -measuring electronics that reduces the measurement resolution without further increase in clock speed. Our scheme adopts a super-heterodyne technique that lowers the original beat frequency to a level of 1 MHz by mixing it with a stable reference signal generated from a special phase- locked-loop. The technique enables us to measure the phase of Doppler shift with a resolution of 1.58 nanometer at a sampling rate of 1 MHz. To avoid the undesirable decrease in the maximum measurable speed caused by the lowered beat frequency, a special form of frequency up-down counting technique is combined with the super-heterodyning. This allows performing required phase unwrapping simply by using programmable digital gates without 2n ambiguities up to the maximum velocity guaranteed by the original beat frequency.

• Archives of Pharmacal Research
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• 제27권6호
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• pp.633-639
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• 2004

The expression of therapeutic transgenes in recombinant adenoviral vectors is a major cause of toxicity in dividing cancer cells as well as non dividing normal cells. To solve the problem of toxicity to normal cells, we have reported on a recombinant adenoviral vector system （AdLP-） in which the expression of the transgene is directed by the tumor-specific L-plastin promoter （LP） （Chung et al., 1999）. The object of this study was to generate a recombinant adenoviral vector system which would generate tumor cell specific expression of cytosine deaminase （CD） gene. We report the construction of a replication-incompetent adenoviral vector in which CD is driven by the L-plastin promoter （AdLPCD）. Infection of 293 cells by AdLPCD generated the functional CD protein as measured by HPLC analysis for the conversion of 5-Fluorocy-tosine （5-FC） to 5-Fluorouracil （5-FU）. HPLC analysis in conjunction with counting radioactivity for ［6-$^3$H］-5FC and ［6-$^3$H］-5FU demonstrated vector dose-dependent conversion of 5-FC to 5-FU in AdLPCD infected ovarian cancer cells. The results from present and previous studies（Peng et al., 2001； Akbulut et al., 2003） suggest that the use of the AdLPCD／5-FC system may be of value in the treatment of cancer including microscopic ovarian cancer in the peritoneal cavity.

• Transactions on Electrical and Electronic Materials
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• 제8권4호
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• pp.166-169
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• 2007

In applying LCD to TV application, one of the most significant factors to be improved is image sticking on the moving picture. LCD is different from CRT in the sense that it's continuous passive device, which holds images in entire frame period, while impulse type device generate image in very short time. To reduce image sticking problem related to hold type display mode, we made an experiment to drive TN-LCD like CRT. We made articulate images by turn on-off backlight, and we realized the ratio of Back Light on-off time by counting between on time and off time for video signal input during 1 frame (16.7 ms). Conventional CCFL (cold cathode fluorescent lamp) cannot follow fast on-off speed, so we evaluated new fluorescent substances of light source to improve residual light characteristic of CCFL. We realized articulate image generation similar to CRT by CCFL blinking drive and TN-LCD overdriving. As a result, reduced image sticking phenomenon was validated by naked eye and response time measurement.

• JSTS:Journal of Semiconductor Technology and Science
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• 제11권2호
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• pp.73-79
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• 2011

This paper describes a reset-free delay-locked loop (DLL) for a memory controller application, with the aid of a hysteresis coarse lock detector. The coarse lock loop in the proposed DLL adjusts the delay between input and output clock within the pull-in range of the main loop phase detector. In addition, it monitors the main loop's lock status by dividing the input clock and counting its multiphase edges. Moreover, by using hysteresis, it controls the coarse lock range, thus reduces jitter. The proposed DLL neither suffers from harmonic lock and stuck problems nor needs an external reset or start-up signal. In a 0.13-${\mu}m$ CMOS process, post-layout simulation demonstrates that, even with a switching supply noise, the peak-to-peak jitter is less than 30 ps over the operating range of 500-1200 MHz. It occupies 0.04 $mm^2$ and dissipates 16.6 mW at 1.2 GHz.

• 자동차안전학회지
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• 제11권2호
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• pp.29-34
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• 2019

This paper presents a new method for construction of a static obstacle map. A static obstacle is important since it is utilized to path planning and decision. Several established approaches generate static obstacle map by grid method and counting algorithm. However, these approaches are occasionally ineffective since the density of LiDAR layer is low. Our approach solved this problem by applying probability theory. First, we converted all LiDAR point to Gaussian distribution to considers an uncertainty of LiDAR point. This Gaussian distribution represents likelihood of obstacle. Second, we modeled dynamic transition of a static obstacle map by adopting the Hidden Markov Model. Due to the dynamic characteristics of the vehicle in relation to the conditions of the next stage only, a more accurate map of the obstacles can be obtained using the Hidden Markov Model. Experimental data obtained from test driving demonstrates that our approach is suitable for mapping static obstacles. In addition, this result shows that our algorithm has an advantage in estimating not only static obstacles but also dynamic characteristics of moving target such as driving vehicles.

• 대한건축학회논문집:계획계
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• 제35권9호
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• pp.41-48
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• 2019

Recently as the rapid increasing of the elderly, silver housing has grown up. Though much fire evacuation and safety law, fire accident is most dangerous problem in our society. So this study is the purpose to analyze evacuation safety in urban silver housing of floor planning by corridor types using human behavior simulation. The methodology of this study is 'Literature Review' and 'Simulation'. This study has been carried out on silver housing's definition, types, fire safety theory and relation law. To proof evacuation safety, this study measured escaping time, longest distance, and bottleneck counting using human behavior simulation. This study use game engine simulation program to analyze 6 corridor types experimental model. As the result of simulation, this study compare between ASET and simulation result. The result come down to 3 part. First, double loaded corridor type is the most dangerous on urban silver housing. Second, Safe shelter's location and number cause increasing of escaping time. Lastly escaping time is influenced by behavior of agent, bottle neck strike frequency.

• 한국가정과교육학회지
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• 제8권1호
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• pp.33-49
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• 1996

The objectives of this study were (1) to determine the preferences for three home economics curriculum Models(Concept-based curriculum Model, Competency-based curriculum Model, and Practical Problem-based curriculum Model) of Korean home economics(HE) teachers and HE teacher educators, (2) to determine the difference between HE teachers and HE teacher educators according to purposes of HE, focus of learning, organization of HE subject matter, focus of HE curriculum, focus of HE content, HE knowledge, main questions addressed through HE curriculum, teaching strategies, students’progress, and systems of action, and (3) to determine the relationships between preferences for three curriculum Models and personal and professional characteristics of HE teachers. Respondents in this study were 225 HE teachers and 35 HE teacher educators. The survey method was used in this descriptive study. The survey method was used in this descriptive study. The overall curriculum Model preference of each teacher respondent was determined by counting number of times a given Model among 10 identified variables. The data were analyzed by using Chi-square to compare the differences between the two groups. To determine the relationships between preferences for three curriculum Models and personal and professional characteristics of HE teachers, coefficient of contingency tables was used. Both of HE teacher group(79.4%) and HE teacher educator group(67.6%) preferred the practical problem-based curriculum Model the most. There was a difference between the two groups on preferences for the curriculum Models related to systems of action. No significant difference emerged when Chi-square was applied to determine difference between the two groups on overall preferences for three HE curriculum Model. The chi square values between preferences for three curriculum Model and level of school, type of school were statistically significant. Each contingency coefficient for level of school(middle school and high school) and form of school(private and public school) was 27, which means there is a low association between the preferences and level of school and the preferences and form of school.

• 한국식품위생안전성학회:학술대회논문집
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• 한국식품위생안전성학회 1996년도 제11회 학술대회 및 정기총회 - 식품의 위생 안전성에 관한 최근 연구 동향
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• pp.33-33
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• 1996

Interests in the field of rapid methods and automation in microbiology have been growing steadily on an international scale in recent years. International meetings concerned this problem have been held in elsewhere in the world countries since the past twenty years. But, unfortunately in the field of microbial examination in food hygiene, this problem have not yet been developed so much as in the field of clinical microbiology. Today, I would like to introduce you here present aspects of rapid and automation technologies, those which are manly carrying in milk and meats industries. My illustration will be given recent improved technologies using automatic apparatus and instruments along with process of microbial count procedure. Recent direct microbiological counting system (ChemeScan \ulcorner) as real time ultrasensitive analysis created by Cheminex Ltd., France is now most evolutional instrument to provide direct microbial counts, down to one cell, within 30 minutes. The results from these evaluations how a good correlation between the ChemScan system and the standard plate count method. This system will be successful application for not only in the field of pharmacology but also food microbiology. In addition, current identification of microbes by sophisticated instruments suitable for food microbiology, one of which Biology is manual system (BIOLOG\ulcorner), provides reference-level capability at a modes price. For the manual system, the color reactions in the microplate are read by eye and manually keyed into personal computer. Species identification appears on the computer screen within seconds, along with biotype patterns, a list of closely related species, and other useful statistics. In present this is useful application for microbial ecology and epidemiological survey. RiboPrinter system newly produced by DuPont is now focusing among microbiologists in the world, and is one of the biggest microbial characterization system using a DNA-based approach. The technology analyzer is bacterial culture for its genetic fingerprint or riboprint pattern. Finally Bio-cellTracer system for automatic measurement of fungal growth and Fukitori-Maseter, a Surface Hygiene Monitoring Kit by using swabe procedure in food processing environment are briefly illustrated in this presentation.

• 사물인터넷융복합논문지
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• 제8권5호
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• pp.77-83
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• 2022

낸드 플래시 메모리는 구조적으로 쓰기 전 지우기(Erase-Before-Write) 동작이 요구된다. 이것을 해결하기 위해서는 데이터 업데이트 동작이 빈번히 발생하는 페이지(Hot data page)를 구분하여 별도에 블록에 저장함으로 해결할 수 있으며 이러한 Hot data를 분류하는 기법을 핫 데이터 판단기법이라 한다. MHF(Multi Hash Function Framework)기법은 데이터 갱신요청의 빈도를 시스템 메모리에 기록하고 그 기록된 값이 일정 기준 이상일 때 해당 데이터 갱신요청을 Hot data로 판단한다. 하지만 데이터 갱신요청에 빈도만을 단순히 카운트하는 방법으로는 정확한 Hot data로 판단에 한계가 있다. 또한 데이터 갱신요청의 지속성을 판단 기준으로 하는 기법의 경우 갱신요청 사실을 시간 간격을 기준으로 순차적으로 기록한 뒤 Hot data로 판단하는 방법이다. 이러한 지속성을 기준으로 하는 방법의 경우 그 구현과 운용이 복잡한 단점이 있으며 갱신요청에 빈도를 고려하지 않는 경우 부정확하게 판단되는 문제가 있다. 본 논문은 데이터 갱신요청에 빈도와 지속성을 함께 고려한 경량화된 핫 데이터 판단기법을 제안한다.