Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Lentivirus-mediated Gene Transfer to Bovine Embryos

  • Kim, Young-Mi (Sangji Youngseo College) ;
  • Kwon, Mo-Sun (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kim, Teo-An (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Yom, Heng-Cherl (Department of Life Science, Hoseo University) ;
  • Ko, Dae-Hwan (Sangji Youngseo College)
  • Published : 2008.03.31
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Abstract

Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.

Keywords

References

  1. Brinster RL, Chen HY, Trumbauer M, Senear AW, Warren R, Palmiter RD (1981): Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene into eggs. Cell 27:223-231 https://doi.org/10.1016/0092-8674(81)90376-7
  2. Cabot RA, Kuhholzer B, Chan AW, Lai L, Park KW, Chong KY, Schatten G, Murphy CN, Abeydeera LR, Day BN, Prather RS (2001): Transgenic pigs produced using in vitro matured oocytes infected with a retroviral vector. Anim Biotechnol 12:205-214 https://doi.org/10.1081/ABIO-100108347
  3. Chan AWS, Homan EJ, Ballou LU, Burns JC, Bremel DR (1998): Transgenic cattle produced by reversetranscribed gene transfer in oocytes. 95:14028-14033 https://doi.org/10.1073/pnas.95.24.14028
  4. Cherry SR, Biniszkiewicz D, van Parijs L, Baltimore D, Jaenisch R (2000): Mol Cell Biol 20:7419-7426 https://doi.org/10.1128/MCB.20.20.7419-7426.2000
  5. Covarrubias L, Nishida Y, Mintz B (1986): Early postimplantation embryo lethality due to DNA rearrangement in a transgenic mouse strain. c 83:6020-6024 https://doi.org/10.1073/pnas.83.16.6020
  6. Goff SP (2001): Retroviridae: The retroviruses and their replication. In: Knipe DM, Howley PM (eds.). Fields Virology. 4th ed. Lippincott Williams and Wilkins, Philadelphia, pp 1871-1939
  7. Gordon JW, Scangos GA, Plotkin DJ, Barbosa JA, Ruddle FH (1980): Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci USA 77:7380-7384 https://doi.org/10.1073/pnas.77.12.7380
  8. Hofmann A, Kessler B, Ewerling S, Weppert M, Vogg B, Ludwig H, Stojkovic M, Boelhauve M, Brem G, Wolf E, Pfeifer A (2003): Efficient transgenesis in farm animals by lentiviral vectors. EMBO Rep 4:1054-1060 https://doi.org/10.1038/sj.embor.7400007
  9. Hofmann A, Zakhartchenko V, Weppert M, Sebald H, Wenigerkind H, Brem G, Wolf E, Pfeifer A (2004): Generation of transgenic cattle by lentiviral gene transfer into oocytes. Biol Reprod 71:405-409 https://doi.org/10.1095/biolreprod.104.028472
  10. Jaenisch R (1976): Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proc Natl Acad Sci USA 73:1260-1264 https://doi.org/10.1073/pnas.73.4.1260
  11. Jaenisch R, Mintz B (1974): Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci USA 71:1250-1254 https://doi.org/10.1073/pnas.71.4.1250
  12. Kim T, Leibfried-Rutledge ML, First NL (1993): Gene transfer in bovine blastocysts using replication-defective retroviral vectors packaged with Gibbon ape leukemia virus envelopes. Mol Reprod Dev 35:105-113 https://doi.org/10.1002/mrd.1080350202
  13. Mahon KA, Overbeek PA, Westphal H (1988): Prenatal lethality in a transgenic mouse line is the result of a chromosome translocation. Proc Natl Acad Sci USA 85:1165-1168 https://doi.org/10.1073/pnas.85.4.1165
  14. Massey JM (1990): Animal production industry in the year 2000 A.D. J Reprod Fert Suppl 41:199-208
  15. Parrish JJ, Susko-Parrish JL, Leibfried-Rutledge ML, Cristser ES, Eyestone WH, First NL (1986): Bovine in vitro fertilization with frozen-thawd semen. Theriogenology 25:591-600 https://doi.org/10.1016/0093-691X(86)90143-3
  16. Pfeifer A, Verma IM (2001a): Gene therapy: promises and problems. Annu Rev Genomics Hum Genet 2:177-211 https://doi.org/10.1146/annurev.genom.2.1.177
  17. Pfeifer A, Verma IM (2001b): Virus vectors and their applications. In: Knipe DM, Howley PM (eds.). Fields Virology. 4th ed. Lippincott Williams and Wilkins, Philadelphia, pp 469-491
  18. Popa I, Harris ME, Donello JE, Hope TJ (2002): CRM1-dependent function of a cis-acting RNA export element. Mol Cell Biol 22:2057-2067 https://doi.org/10.1128/MCB.22.7.2057-2067.2002
  19. Pursel VG, Pinkert CA, Miller KF, Bolt DJ, Campbell RG, Palmitter RD, Brinster RL, Hammer RE (1989): Genetic engineering of livestock. Science 244:1281-1288 https://doi.org/10.1126/science.2499927
  20. Rosenkrans CF Jr, Zeng GQ, Mcnamara PK, Schoff PK, First NL (1993): Development of bovine embryos in vitro as affected by energy substrates. Biol Reprod 49:459-462 https://doi.org/10.1095/biolreprod49.3.459
  21. Sauter SL, Gasmi M (2001): FIV Vector Systems. Somat Cell Mol Genet 26:99-129 https://doi.org/10.1023/A:1021078714105
  22. Saeki K, Hoshi M, Leibfried-Rutledge ML, First NL (1990): In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone. Theriogenology 34:1035-1039 https://doi.org/10.1016/S0093-691X(05)80002-0
  23. Sirard MA, Parrish JJ, Ware CB, Liebfried-Rutledge ML, First NL (1988): The culture of bovine oocytes to obtain developmentally competent embryos. Biol Reprod 39:546-552 https://doi.org/10.1095/biolreprod39.3.546
  24. Vogt VM (1997): Retroviral virons and genomes. In: Coffin JM, Hughes SH, Varmus HE (eds.). Retroviruses. Cold Spring Harbor Lab. Press, Plainview, NY, pp 27-69
  25. Wall RJ (1996): Transgenic livestock: progress and prospects for the future. Theriogenology 45:57-68 https://doi.org/10.1016/0093-691X(95)00355-C
  26. Walton JR, Murray JD, Marshall JT, Nancarrow CD (1987): Zygote viability in gene transfer experiments. Biol Reprod 37:957-967 https://doi.org/10.1095/biolreprod37.4.957
  27. Whitelaw C, Radcliffe P, Ritchie W, Carlisle A, Ellard F, Pena R, Rowe J, Clark A, King T, Mitrophanous K (2004): Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector. FEBS Letters 571:233-236 https://doi.org/10.1016/j.febslet.2004.06.076
  28. Zufferey R, Donello JE, Trono D, Hope TJ (1999): Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J Virol 73:2886-2892