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Lentivirus-mediated Gene Transfer to Bovine Embryos  

Kim, Young-Mi (Sangji Youngseo College)
Kwon, Mo-Sun (Department of Physiology, Catholic University of Daegu School of Medicine)
Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine)
Kim, Teo-An (Department of Physiology, Catholic University of Daegu School of Medicine)
Yom, Heng-Cherl (Department of Life Science, Hoseo University)
Ko, Dae-Hwan (Sangji Youngseo College)
Publication Information
Reproductive and Developmental Biology / v.32, no.1, 2008 , pp. 15-20 More about this Journal
Abstract
Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.
Keywords
Bovine embryos; Gene transfer; Lentivirus-based vector; Enhanced green fluorescent protein (EGFP) gene;
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