• 대한한의정보학회지
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• 제15권1호
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• pp.77-84
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• 2009

Objectives : The aim of the present study is to evaluate the effects Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae extracton 3T3-L1 preadipocytes differentiation. Methods : We adopted Oil Red O staining methods to observe the formed lipid droplets. And the amount of lipids in adipocytes was measured using absorptiometric analysis. Results : The extracts of Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae stimulated the preadipocytes differentiation and lipid droplet formation. And the complex extract of the traditional herbal medicines stimulated there actions more than single extracts. Conclusions : The extract of Rehmannia glutinosa, Radix puerariae, Semen coicis, Fructus hordei, Cortex acanthopanacis, Fructus chaenomelis, or Radix glycyrrhizae has stimulatory effects. on adipogenesis. Moreover, the complex extract of the traditional herbal medicines has more effect than single extracts.

• Journal of Ginseng Research
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• 제46권4호
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• pp.601-608
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• 2022

Background: Various treatments are used to relieve menopausal symptoms for women. However, herbal substances are frequently used as complementary and alternative therapies as other treatments can increase ovarian and breast cancer risk. While the herbal substances' therapeutic effect is essential, the safety of their use is considered more important. This study aims to confirm the safety of red ginseng and herb extract complex (RHC), which are used to relieve menopausal symptoms. Methods: This randomized, double-blind, placebo-controlled clinical study recruited and divided 120 women experiencing menopausal symptoms into the RHC and placebo groups (60 women per group). Subjects were administered with 2 g RHC or placebo daily for 12 wk. Adverse reactions, female hormonal changes, and uterine thickness were observed and recorded on wk 0, 6, and 12. Hematologic and blood chemistry tests were also conducted. Results: The reactions of the subjects who received RHC or placebo at least once were analyzed. A total of six adverse reactions occurred in the RHC group, while nine occurred in the placebo group; common reactions observed in both groups were genital, subcutaneous tissue, and vascular disorders. However, there was no statistically significant difference between the administration groups (p = 0.5695), and no severe adverse reactions occurred in both groups.

• 한국발생생물학회지:발생과생식
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• 제5권2호
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• pp.123-129
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• 2001

포유동물의 난포내 난자의 성숙 시에는 난자를 둘러싸고 있는 난구세포의 확장 현상이 일어나는데 이 현상에는 hyaluronic acid 뿐만 아니라 다른 성분도 관여하는 것으로 알려져 있다. 본 연구는 조직 재구성 과정에서 중요한 역할을 하는 matrix metalloproteinase(MMP)가 사람의 성숙한 난자-난구 복합체의 extracellular matrix(ECM)에 존재하는지의 여부를 알아보고자 하였다. 체외수정 시술 시에 얻어지는 사람의 난자-난구 복합체를 재료로 zymography와 western blotting 방법으로 조사한 결과 난자-난구 복합체의 ECM에는 300kDa, 240kDa, 200kDa, 180kDa, 116kDa, 97kDa, 그리고 84kDa의 분자량을 갖는 적어도 7종류의 gelatinase들이 존재하는 것이 관찰되었다. 이들 gelatinase가 MMP인지를 확인하기 위해 zymography 동안에 ethylenediaminetetraacetic acid 혹은 phenanthroline 등의 MMP 억제제를 처리한 결과 7종류 모두의 gelatinase 효소활성이 사라졌다. 또한 MMP의 활성제인 aminophenylmercuric acetate를 zymography를 시행하기 전에 ECM에 처리한 결과 200kDa, 180kDa, 97kDa, 84kDa의 gelatinase활성이 사라지고, 대신에 80kDa, 65kDa, 60kDa의 분자량을 갖는 새로운 gelatinase 단백질의 효소활성이 나타났다. 이로 미루어 사람 난자-난구 복합체의 ECM에는 여러 종류의 gelatinase들이 있으며 이들 중 일부는 MMP-2와 MMP-9의 동위효소들인 것으로 여겨진다.

• 한국실내디자인학회논문집
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• 제24권6호
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• pp.30-37
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• 2015

This study has the purpose to investigate the landscape plans focused on the public sector housing complexes in the Seoul Metropolitan Area in the 2000s. The targeted subjects of this study are 24 districts conducted by the Corporation, and the spatial extent is limited to Seoul metropolitan area. The scope of the research is limited to the public sector, because this sector has been conducted preferentially for public needs rather than the development profit, and has positively reflected the will "pre plan - post development". In view of the study methodologies, this study examined the transition process in Korea housing complex and analyzed the design reports to extract the key planning concepts and planning methods of landscape planning. The main concepts of landscape planning analyzed in this study were urban landscape, natural landscape, streetscape, architectural landscape, axis for viewing, landmark, skyline and landscape by areas. The key planning methods of landscape planning were landmark, which is a point landscape element, axis for viewing and skyline, which are linear landscape elements, landscape by areas which is an area landscape element, and finally complex landscape, walking landscape and nightscape, which are three-dimensional landscape elements.

• 한국미생물·생명공학회지
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• 제22권6호
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• pp.614-620
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• 1994

The effects of growth temperature and nutritional components on the synthesis of poly-3-hydroxybutyric acid, PHB, by filamentation-suppressed recombinant Escherichia coli XL1-Blue (pSYL107) were studied. After culturing XL1-Blue(pSYL107) for 48 hours in complex medium at 30$\circ$C, 7Al g/l of PHB could be obtained with the PHB content and PHB yield of 82% and 0.371 g PHB/g glucose, respectively. Lower concentration of PHB(3.2 g/l) was obtained when cultu- red at 37$\circ$C, which seemed to be due to the instability of this strain having amplified FtsZ activity. The PHB concentration of 3.75 g/l was obtained after culturing 60 hours in R medium supplemen- ted with 20 g/l glucose at 30$\circ$C, which was more than twice higher than that obtained with XL1-Blue(pSYL105). This suggested that the enhancement of PHB synthesis by suppressing filamenta- tion was more significant in a defined medium than complex medium. PHB synthesis could be further enhanced by supplementing a small amount of various complex nitrogen sources. When 5 g/l of beef extract was added to a defined medium, PHB concentration, PHB content, and PHB yield obtained after 60 hours of cultivation at 30$\circ$C were 7.46 g/l, 86%, and 0.375 g PHB/g glucose,respectively.

• 대한화학회지
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• 제7권2호
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• pp.170-173
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• 1963

A tri-thiocyanate molybdenum (Ⅴ) complex containing pyridine has been prepared by one step process; namely, the solvent extraction of molybdenum (Ⅴ)-thiocyanate complex into organic solvents followed by precipitation of the compound by addition of pyridine to the extract. It is concluded that the compound has a definite composition regardless of the various mole ratios of molybdenum to thiocyanate ion employed in the preparation. The use of hydrazine as the reducing agent eliminates the necessity of working under inert atmosphere and of further purification of the product. Molybdenum (Ⅴ)-thiocyanate (1:3) complex can be quantitatively and selectively extracted with slightly polar organic solvents such as ethyl acetate, and the pyridine complex is quantitatively obtained due to the insolubility of the compound in them. The oxidation state of molybdenum in the compound is + 5 and the formula appears to be $MoH_2O_2(NCS)_3Py_2$ from the contents of Mo and NCS in the compound. The solubility of the compound in various has been studied at $25^{\circ}C$.

• 한국광학회지
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• 제20권6호
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• pp.328-333
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• 2009

본 논문에서는 복소수 홀로그램을 탈 축 홀로그램으로 변환해 쌍 영상 잡음 없이 3차원 홀로그램 디스플레이를 구현하는 방법 을 제안하고 실연하였다. 이러한 기술을 구현하기 위해, 서로 다른 깊이에 위치하는 슬라이드 패턴으로 구성된 3차원 물체의 복소수 홀로그램을 광 스캐닝 홀로그램 시스템을 이용해 추출한 후, 추출된 복소수 홀로그램에 공간 캐리어를 수치적인 방법으로 인가하고 실수 부분만을 추출해 복소수 홀로그램을 탈 축 홀로그램으로 변환한다. 변환된 탈 축 홀로그램을 진폭만을 변조하는 공간 광 변조기에 인가하고, 시준된 레이저 빔을 공간 광 변조기에 투사하여 탈 축 홀로그램을 복원하는 방식으로 3차원 디스플레이를 구현한다.

• Journal of Applied Biological Chemistry
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• 제59권1호
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• pp.37-43
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• 2016

Bioactive components of ultra-fine ground Saururus, the extraction yield increases when the leaves are ultra-fine ground. Comparison of normal-ground and ultra-fine ground Saururus chinensis leaves showed that the solid content and antiinflammatory activity of ultra-fine ground extracts was higher than that of normal-ground extracts. Lipopolysaccharide (LPS)-stimulated Raw 264.7 cells were treated with different concentrations of Saururus chinensis extract and the amount of nitric oxide (NO) was determined; LPS-treated cells produced 2 times more NO than cells that were not treated with LPS. Moreover, the NO production in cells treated with Saururus chinensis extract was inhibited in a concentration-dependent manner. Because the stimulant-induced NO production is regulated by the inducible nitric oxide synthase (iNOS), we measured the iNOS protein level to elucidate the mechanism by which the NO production was inhibited. We found that the amount of iNOS decreased dose-dependently. It was reduced by 53% at a Saururus chinensis extract concentration of $100{\mu}g/mL$. The protein expression of cyclooxygenase-2 (COX-2) in LPS-treated Raw 264.7 cells was inhibited by 31% at $100{\mu}g/mL$ of Saururus chinensis extract. Gel shift of the nuclear factor kappa B-DNA complex occurred in LPS-treated cells and the intensity of the band decreased gradually in a concentration-dependent manner. Ultra-fine ground Saururus chinensis extract had a concentration-dependent inhibitory effect on the production of prostaglandin $E_2$, tumor necrosis factor ${\alpha}$, interleukin $1{\beta}$ (IL-$1{\beta}$), IL-6, and IL-8 in LPS-treated Raw 264.7 cells, i.e., at $50{\mu}g/mL$ of Saururus chinensis extract, their levels were decreased by 53, 67, 52, 37, and 21% respectively.

• 생명과학회지
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• 제21권6호
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• pp.875-883
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• 2011

마늘추출물 및 비타민 B군 강화 식이를 4주간 급이한 후 흰쥐에 수영부하 실험으로 스트레스를 가하였을 때, 피로회복 인자와 관련된 흰쥐의 체내 지질성분 및 간 기능에 미치는 영향을 분석하였다. 혈당은 대조군($193.41{\pm}3.85$ mg/dl)보다 실험식이군이 $152.85{\pm}6.57{\sim}169.93{\pm}8.75$ mg/dl로 유의적으로 낮았다. 혈청 중 총 지질, 총 콜레스테롤 및 LDL-콜레스테롤은 대조군에 비해 낮은 경향이었으며 마늘추출물과 비타민 B군 강화 시료를 각각 첨가한 SG군과 SV군 보다는 이들을 혼합 급이한 SGV군에서 유의적으로 더 낮았다. 혈청 중의 TBARS 함량은 정상군에 비해 대조군에서 17.25%나 증가하였으며 마늘추출물 및 비타민 B군 강화 급이로 낮아지는 경향이었다. 간조직의 지질 성분 중 총지질은 정상군에 비해 SGV군이 10% 더 낮은 함량이었고, 중성지질은 대조군에 비해 SGV군에서 46% 더 낮게 정량 되었다. 수영부하 스트레스에 의한 간조직 중의 글리코겐 함량은 대조군이 정상군보다 유의적으로 낮았으며 SG, SV 및 SGV 군은 정상군과 유의차가 없었다. 이상의 결과를 종합하여 볼 때 마늘추출물과 비타민 B군 강화급이는 혈중 혈당, 총콜레스테롤 및 LDL-콜레스테롤을 유의적으로 감소시켰으며 또한 수영부하 스트레스로 혈중 및 간장에 다량 생성될 수 있는 지질과산화물 함량도 유의적으로 감소시키므로 피로회복 및 스트레스 개선에 효과가 있을 것으로 판단된다.

• 대한본초학회지
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• 제38권1호
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• pp.11-19
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• 2023

Objectives : Neuroinflammation is a common pathological mechanism of neurodegenerative diseases, and the development of therapeutic agents is urgently needed. Red ginseng has been known to be good for the immune stimulation in Eastern Asia. Although the immuno-stimulatory activity of red ginseng are already known, the neuro-protective effects of cultivated red ginseng with fermented complex mushroom-cereal mycelium (RGFM) have not been conducted. Thus, in this study, we tried to investigate the anti-neuroinflammatory effect of RGFM water extract on lipopolysaccharide (LPS) stimulated BV2 cells. Methods : BV2 cells were pretreated with RGFM 1 h prior to LPS exposure. To determine the neuro-protective effects of RGFM water extract, we measured the expression of inflammatory mediators including inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and nitric oxide (NO) and pro-inflammatory cytokines such as interleukin (IL)-1𝛽, IL-6 and tumor necrosis factor (TNF)-𝛼 in LPS-stimulated BV2 cells. In addition, to find out the regulatory mechanism of RGFM water extract, we assessed the protein levels of mitogen-activated protein kinases (MAPKs) and inhibitory 𝜅B𝛼 (I𝜅B𝛼) by western blotting. Results : In our study, treatment of RGFM reduced the mRNA expression of iNOS and COX-2 and suppressed NO production in LPS-stimulated BV2 cells. Additionally, the secretion of IL-1𝛽 and TNF-𝛼 but not IL-6 was significantly inhibited by RGFM. Furthermore, RGFM water extract inhibited the phosphorylation of c-Jun N-terminal kinase (JNK). Conclusions : Taken together, these findings suggest that RGFM water extract has a protective effect on neuroinflammation through inhibition of JNK.