• Journal of Microbiology and Biotechnology
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• 제28권2호
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• pp.181-189
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• 2018

Candida albicans is a major pathogenic fungus in humans, and meets at first the innate immune cells, such as macrophages, in its host. One important strategy of the host cell to kill C. albicans is to produce reactive oxygen species (ROS) by the macrophages. In response to ROS produced by the macrophages, C. albicans operates its defense mechanisms against them by expressing its oxidative stress response genes. Although there have been many research studies explaining the specific transcription factors and the expression of the oxidative stress genes in C. albicans, the regulation of the oxidative stress genes by chromatin structure is little known. Epigenetic regulation by the chromatin structure is very important for the regulation of eukaryotic gene expression, including the chromatin structure dynamics by histone modifications. Among various histone modifications, histone acetylation is reported for its direct relationship to the regulation of gene expression. Recent studies reported that histone acetyltransferases regulate genes to respond to the oxidative stress in C. albicans. In this review, we introduce all histone acetyltransferases that C. albicans contains and some papers that explain how histone acetyltransferases participate in the oxidative stress response in C. albicans.

• Archives of Pharmacal Research
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• 제27권10호
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• pp.1060-1064
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• 2004

Although many studies have been performed to elucidate the molecular consequences of ultraviolet irradiation, little is known about the effect of natural products. Ultraviolet irradiation is widely considered to be an environmental stress. Here we investigated the effect of 3,23-dihydroxy- 20(29)-lupen-27-oic acid on the regulation of MMP-1 and type 1 procollagen in Ultraviolet irradiation of cultured old age human dermal fibroblasts. 3, 23-dihydroxy-20(29)-lupen-27- oic acid was isolated from Tiarella polyphylla D. Don (Saxifragaceae). Among them, 3, 23- dihydroxy-20(29)-lupen-27-oic acid induced the regulation of Type 1- procollagen and reduced the regulation of MMP-1 at the protein levels in a dose-dependent manner by ultraviolet irradiation. Taken together, our results suggest that 3, 23-dihydroxy-20(29)-lupen-27-oic acid plays an important role in the induction of Type 1-procollagen and reduction of MMP-1 by ultraviolet irradiation in old age human dermal fibroblasts.

• 대한한의학방제학회지
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• 제32권1호
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• pp.39-49
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• 2024

Objectives : The purpose of this study was to investigate the molecular targets and pathways of anti-inflammatory effects of Jakyakgamchotang with corydalis tuber (JC) using network pharmacology. Methods : The compounds in constituent herbal medicines of JC were searched in TCM systems pharmacology (TCMSP). Target gene informations of the components were collected using chemical-target interactions database provided by Pubchem. Afterwards, network analysis between compounds and inflammation-related target genes was performed using cytoscape. Go enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed on inflammation-related targets using DAVID database. Results : 70 active compounds related to inflammation were identified, and 295 target genes related to the anti-inflammatory activity of the compound of JC were identified. In the Go biological process DB and KEGG pathway DB, "inflammatory response", "cellular response to lipopolysaccharide", "positive regulation of interleukin-6 production", and "positive regulation of protein kinase B. signaling", "positive regulation of ERK1 and ERK2 cascade", "positive regulation of I-kappaB kinase/NF-kappaB signaling", "negative regulation of apoptotic process", and "PI3K-Akt signaling pathway" were found to be mechanisms related to the anti-inflammatory effects related to the target genes of JC. The main compounds predicted to be involved in the anti-inflammatory effect of JC were quercetin, licochalcone B, (+)-catechin, kaempferol, and emodin. Conclusions : This study provides the molecular targets and potential pathways of JC on inflammation. It can be used as a basic data for using JC for various inflammatory disease in traditional korean medicine clinic.

• 아동학회지
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• 제30권1호
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• pp.43-57
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• 2009

This study examined relationships between caregivers' attachment, young children's emotion regulation, and aggression. Subjects were 110 institutionalized and 105 home-reared children (M=3.8 and 4.2 years, respectively). Data were collected from children's caregivers via questionnaires and analyzed by ANOVA, correlations, and hierarchical regressions. Results showed that (1) caregivers' attachment and the emotion regulation and aggression of institutionalized children were significantly different from those of home-reared children; (2) caregiver's attachment was positively related to emotion regulation for both institutionalized and home-reared children; (3) for institutional children, negative relationships between attachment and aggression was found for girls; negative relationships between emotion regulation and aggression was found for boys. These findings underscore negative effects of institutionalized care on children's socio-emotional behaviors.

• 감성과학
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• 제17권1호
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• pp.63-70
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• 2014

Emotion regulation has been suggested as a pathway through which personality traits (e.g., extraversion or neuroticism) affect psychological well-being. However, the pathway needs further investigation across cultures due to variations in parts of the relationship reported in recent culture research. With an aim of improving current understanding of the pathway, we investigated the role of emotion regulation mediating the link between personality traits and well-being across two college samples from different cultural backgrounds: Korea and the United States (US). Results of mediation analysis revealed that the extraversion-well-being relationship was fully mediated by the degree to which individuals regulate negative emotions in both Korean and US samples. However, the neuroticism-well-being relationship was partially mediated by emotion regulation in the US sample, while it was fully mediated in the Korean sample. The role of emotion regulation differently functioning across cultures suggests the importance of investigating cultural-specific mechanism of psychological processes.

• 대한방사성의약품학회지
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• 제1권2호
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• pp.98-103
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• 2015

Good manufacturing Practice (GMP) regulation for diagnostic and therapeutic radiopharmaceuticals was prepared at 2014. The mandatory GMP regulation becomes effective on $1^{st}$, July 2015,with two years of grace periods. Korean radiopharmaceuticals GMP regulation was consisted of quality management, personnel, premise and facility, documentation, production, quality control and self-audit and they have a very similar structure to European Union and PIC/S GMP regulation. Here, we describe detailed description of GMP regulation each part and application to radiopharmaceuticals production. And we also compare Korea, Japan and USA radiopharmaceuticals GMP regulation. GMP is a method to maintain quality of radiopharmaceuticals in daily production and it must be embedded on the manufacturing operation and management.

• 한국직업건강간호학회지
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• 제22권3호
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• pp.257-265
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• 2013

Purpose: This study aimed to investigate the job stress level of call center workers and figure out its relation with cognitive emotion regulation strategies as a cognitive coping strategy. Methods: Data collection was conducted on 985 call center workers, working for K companies in Korea. ANOVA, t-test, Scheffe's test and multiple regression have been conducted for the data analysis using SAS 9.0 software. Results: The total job stress score of subjects ranges $Q_{25{\sim}49}$ (the lower 50%) whereas the score of job demand and job control ranges $Q_{50{\sim}74}$ (the upper 50%), and its sectional job stress level is high. Since job stress is the major factor affecting cognitive emotion regulation strategies, it is shown that when the job stress score gets higher, the score of positive cognitive emotion regulation strategies gets decreased but the score of negative cognitive emotion regulation strategies, increased. Conclusion: Based on the results of this study, it is necessary to develop and apply an effective stress easing method for call center workers which reduces negative cognitive emotion regulation strategies and increases positive ones.

• 한국생활과학회지
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• 제24권5호
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• pp.645-661
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• 2015

The purpose of this study was to investigate the effects of socio-demographic variables, adult attachment and emotion regulation style on psychological well-being of university students. We conducted a survey on 318 university students in Cheongju-si and Hongseong gun. T-test, Pearson' productive correlation, hierarchical regression analysis were used. The results were as follows: First, there was a significant difference in emotion regulation style according to university student's sex. In other words, females used more support-seeking style than males did. Second, male university students' psychological well-being was high at more active style, less anxiety attachment and avoidant/distractive style, older students, less avoidance attachment, and higher economic status. Third, female university students' psychological well-being was high at less avoidant/distractive style and more active style, older students, less anxiety attachment, and more support-seeking style. In conclusion, emotion regulation style was the strongest factor to increase university student's psychological well-being among other variables. The implications and limitations of this study were discussed.

• 한국융합학회논문지
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• 제9권5호
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• pp.269-279
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• 2018

본 연구는 간호대학생의 긍정심리자본, 사회적 지지 및 정서조절능력 간의 관련성을 파악하고 사회적 지지 및 정서 조절능력이 긍정심리자본에 미치는 영향을 규명하기 위해 실시되었다. 연구방법은 간호대학생 157명을 대상으로 조사연구를 실시하였다. 구조화된 설문지로 자료 수집하였고, SPSS/Win 21.0 program으로 분석하였다. 연구결과 간호대학생의 긍정 심리자본은 중간이상(3.68점/5점 만점)의 수준이었고, 긍정심리자본과 사회적 지지, 정서조절능력 간에는 유의한 정적 상관 관계가 있었다. 분석결과 긍정심리자본에 영향을 미치는 요인은 성별(${\beta}=-.11$), 교수관계(${\beta}=.16$), 평가적 지지(${\beta}=.40$), 자기정서조절(${\beta}=.39$)이었다. 모델의 전체 설명력은 68%였으며, 정서조절능력 중 자기정서조절이 긍정심리자본을 9%설명하는 것으로 나타났다. 간호대학생의 긍정심리자본 증진을 위해 정서조절 프로그램을 개발하고 제공하는 것이 효과적인 전략이 될 수 있다.

• Biomolecules & Therapeutics
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• 제23권1호
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• pp.31-38
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• 2015

Histone acetylation plays a critical role in the regulation of transcription by altering the structure of chromatin, and it may influence the resistance of some tumor cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) by regulating the gene expression of components of the TRAIL signaling pathway. In this study, we investigated the effects and molecular mechanisms of trichostatin A (TSA), a histone deacetylase inhibitor, in sensitizing TRAIL-induced apoptosis in Caki human renal carcinoma cells. Our results indicate that nontoxic concentrations of TSA substantially enhance TRAIL-induced apoptosis compared with treatment with either agent alone. Cotreatment with TSA and TRAIL effectively induced cleavage of Bid and loss of mitochondrial membrane potential (MMP), which was associated with the activation of caspases (-3, -8, and -9) and degradation of poly (ADP-ribose) polymerase (PARP), contributing toward the sensitization to TRAIL. Combined treatment with TSA and TRAIL significantly reduced the levels of the cellular Fas-associated death domain (FADD)-like interleukin-$1{\beta}$-converting enzyme (FLICE) inhibitory protein (c-FLIP), whereas those of death receptor (DR) 4, DR5, and FADD remained unchanged. The synergistic effect of TAS and TRAIL was perfectly attenuated in c-$FLIP_L$-overexpressing Caki cells. Taken together, the present study demonstrates that down-regulation of c-FLIP contributes to TSA-facilitated TRAIL-induced apoptosis, amplifying the death receptor, as well as mitochondria-mediated apoptotic signaling pathways.