• 한국임상수의학회지
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• 제18권4호
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• pp.358-362
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• 2001

Brain dead (BD) patients remain the largest source of solid organs for transplantation. BD has shown to decrease graft function and survival in rodent models. The aim of this study was to evaluate how brain death affects graft viability in the donor and kidney tolerance to cold preservation as assessed by survival in a canine transplantation. 13 Beagle dogs were used for the study. Brain death was induced by the sudden inflation of a subdural balloon catheter with continuous monitoring of arterial blood pressure and eletroencephalographic activity (n=3). Sixteen hours after conformation of brain death, kidney graft were retrieved (n=6). Non-BD donors served as controls (n=4). All kidneys were flushed with University of Wisconsin (UW) solution and preserved for 24 hours at 4$^{\circ}C$ before transplantation. Recipient survival rates, serum creatinine level were analyzed. Brain death induced the well-known Cushing reaction with a severe increase in blood pressure and tachycardia. Thereafter, cardiac function returned progressively to baseline within 8 hours and remained stable until the end of the experiment. All of dogs in both group transplanted were survived until 7 days (100%), and the kidneys showed functional early rejection at 8.3$\pm$0.5 days and 8.5$\pm$0.5 days after transplantation, in BD and allograft group, respectively. BD kidneys were functionally similar to control kidneys for 7 days after transplantated. Brain death has no deleterious effect on preservation injury and survival of dog kidney transplantation, although it induces changes in hemodynamic parameters. This study reveals that kidneys from BD donors do not exhibit more ischemia reperfusion injury, and support good early function and survival.

• 사상체질의학회지
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• 제32권3호
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• pp.64-71
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• 2020

Objectives To report an improved case with Taeeumin Mahwangjeongcheon-tang on the patient with chronic bronchitis caused by an inhalation injury. Methods The patient had been suffered from cough, sputum, dyspnea, and chest discomfort for 15 years. He was diagnosed as Taeeumin Esophageal Cold Pattern and was given Mahwangjeongcheon-tang according to Sasang constitutional medical theory. The clinical asthma measurement scale in oriental medicine(CAMSOM-V), quality of life questionnaire for adult Korean asthmatics(QLQAKA), and Spirometry were performed to evaluate the improvement of his symptoms. Results The clinical asthma measurement scale in oriental medicine(CAMSOM-V) improved from 16 to 14 points and the quality of life questionnaire for adult Korean asthmatics(QLQAKA) improved from 55 to 67 points. In spirometry, forced vital capacity improved from 3.07 to 3.59, and forced expiratory volume in 1sec improved from 2.59 to 2.84 after 4 weeks. Conclusion This report suggests that Mahwangjeongcheon-tang is effective for chronic bronchitis.

• 약학회지
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• 제58권5호
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• pp.300-306
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• 2014

Ethanol consumption causes psychomotor alterations. Hovenia Semen seu Fructus (HS), widely distributed in Korea, China, and Japan, has been reported to have beneficial effects on acute alcohol-induced liver injury. The present study sought to assess the effects of HS extract on ethanol-induced psychomotor alterations in rats. Sprague-Dawley rats were orally (p.o.) given ethanol (4 g/kg) (ethanol group) to induce psychomotor alterations. A separate group (HS-treated groups), were treated with different dosages of HS (50, 100, and 200 mg/kg, p.o.), 30 minutes before ethanol treatment. The control group received only the vehicle (saline). Ethanol-induced psychomotor alterations were evaluated in the open-field, rota-rod, hanging wire, and cold swimming test. In addition, blood ethanol and acetaldehyde concentrations were also measured. Behavioral evaluations and blood analysis were carried out 0.5, 1, 2, 4, and 8 hours after ethanol administration. Pre-treatment of HS ameliorated ethanol-induced alterations in the open-field, rota-rod, and cold swimming test, significantly evident in 2 and 4 hours after ethanol treatment. These improvements coincided with decrease in blood ethanol and acetaldehyde concentration. Based on these results, the present study suggests that HS may have ameliorating effects against ethanol-induced psychomotor alterations.

• The Plant Pathology Journal
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• 제38권4호
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• pp.403-409
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• 2022

In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA- strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.

• 한국작물학회지
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• 제51권spc1호
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• pp.119-124
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• 2006

고품질 쌀 생산을 위한 기초자료를 얻기 위하여 작물과학원 춘천출장소 냉수처리 포장에서 냉해피해 정도가 다른 시료를 채취하여 벼의 도정특성, 쌀의 이화학적특성, 쌀가루의 아밀로그램특성 및 밥의 식미관능검정을 실시한 결과는 다음과 같다. 1. 냉수관개에 의한 냉해 발생은 불임율이 높을수록 출수 지연일수가 길고, 간장의 단축정도는 심했으며, 이삭의 추출은 불량하였다. 2. 쌀의 외관품질은 불임을 20% 이상부터 완전미율이 낮아지고, 유백미율과 깨어진 쌀이 증가하는 경향이었다. 3. 쌀의 이화학적특성은 냉수관개로 냉해를 유발하고 생산된 쌀이 자연구에 비해 단백질 함량이 $0.9{\sim}2.0%$ 높았으며, 백미의 투명도가 떨어지며, 밥의 윤기치도 낮았다. 4. 쌀가루의 아밀로그램특성은 저온피해를 받은 쌀은 호화개시온도가 높고, 최고점도와 강하점도는 낮았으며, 치반점도는 높았다. 5. 식미관능검정에서는 냉해로 인해 불임율이 높을수록 식미관능이 자연구에 비해 많이 떨어졌다. 평가항목 중에서 밥맛의 차이가 큰 것은 밥의 찰기와 식미총평 이었으며, 차이가 적었던 것은 밥의 냄새였다.

• Archives of Plastic Surgery
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• 제32권4호
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• pp.485-490
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• 2005

Fingertip is the end of tactile organ and the part of hand most frequently injured. Fingertip injuries should be evaluated on an individual basis considering patient's overall physical condition, medical history, etiology, time of injury, and anticipated future hand use, and accordingly one of various methods of reconstruction should be selected. Complications after the reconstruction of fingertip injuries have been reported as pain, hypersensitivity, numbness, distal paresthesia, cold intolerance, and atrophy. From January to December 2002, dermofat grafts were performed on 15 patients to correct painful fingertips after injury. The thickness of the soft tissue of fingertip was measured both preoperatively and postoperatively with simple X-ray. To evaluate the improvement of pain, visual analogue scale(VAS) was used through the direct interview with patients. The average of postoperative follow-up period was 10.9 months. The average of increased soft tissue thickness ratio was 88.4%(2.3mm to 3.8mm). The average of preoperative VAS was 7.6, and postoperative VAS was 3. Dermofat graft on fingertip needs a further long-term follow-up study for the absorption ratio of dermofat, however, this procedure is simple and could be done under local anesthesia, and would be a useful alternative procedure to correct painful fingertips with the soft tissue atrophy after injury.

• 대한한방내과학회지
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• 제37권5호
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• pp.847-854
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• 2016

Background and Objective: The Korean agricultural population is now rapidly aging. Older people in rural areas are weak due to work-related musculoskeletal disorders. The aim of this case is to report treatment of injuries to the nerves at the wrist and hand level by Korean medicine. Methods: The patient in this case was a 74-year-old male. He had injured nerves at the right wrist and hand level due to agriculture work. We treated him with acupuncture, herbal medicine, etc. Results: The patient showed the first sign of recovery of his wrist after 13 days treatment. After 37 days of treatment, his wrist movement was restored to a nearly normal range. He showed the first sign of recovery in a finger after 19 days of treatment and his grasping power increased from 0% to 60%. The cold sensation in his hand and arm also disappeared. Conclusions: We cannot generalize based only on this one case study. Nevertheless, this report suggests that Korean medicine may be an effective treatment for injury of nerves at the wrist and hand level.

• The Korean Journal of Pain
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• 제28권2호
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• pp.96-104
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• 2015

Background: The present experiment was conducted to identify the cooperative effect of serine histogranin (SHG) and noradrenaline in alleviating peripheral neuropathic pain. Methods: Chronic constriction injury of the right sciatic nerve was used to induce chronic neuropathic pain. For drug delivery, a PE10 tube was inserted into the subarachnoid space. Acetone drops and a $44^{\circ}C$ water bath were used to evaluate the cold and heat allodynia, respectively. Placing and grasping reflexes were used to assess the locomotor system. Results: SHG at 0.5 and $1{\mu}g$significantly (P < 0.05) decreased the thermal allodynia. The cold allodynia was also significantly reduced by intrathecal injections of 0.5 (P < 0.05) and $1{\mu}g$(P < 0.001) of SHG. $1{\mu}g$of noradrenaline, but not $0.5{\mu}g$, significantly alleviated the cold (P < 0.01) and thermal (P < 0.05) allodynia. The ameliorating effect of noradrenaline or SHG disappeared when the two compounds were administrated in equal concentrations. A significant difference (P < 0.01 in the acetone and P < 0.05 in the heat) was observed in the groups under equal doses of the two compounds, with a lower effectiveness of the combination therapy. Conclusions: Our findings suggest that the simultaneous administrations of noradrenaline and SHG do not result in synergistic analgesia, and combination therapy may not be a good approach to the treatment of chronic neuropathic pain syndrome.

• 한국식품과학회지
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• 제38권1호
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• pp.135-139
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• 2006

세균이 외부stress에 대한 자가저항으로 biofilm형성을 하는 것은 식품 뿐만아니라 식품기기등의 세척, 소독등의 식품안전 확보에 많은 어려움을 주게 된다. 본 연구에서는 glass wool과 mlcrotiter plate assay를 이용하여 주요 식중독 세균인 Salmonella, E. coli, B. cereus, S. aureus를 여러가지 식품보존하에서 상해와 biofilm형성 정도를 비교하였다. 이들 세균은 외부의 stress없는 조건하에서도 상해를 받지 않았고 모두 biofilm이 형성되어 glass wool에 부착되었다. Microtiter plate assay에서의 상해별 biofilm형성은 acid stress에서 10%이내의 상해를 받은 E. coli와 약 40%의 상해를 받은 S. aureus에서 높게 나타났다. $4^{\circ}C$의 cold temperature에서는 30-50% 상해를 나타낸 B. cereus와 E. coli가 높은 biofilm 형성을 보였고 cold starvation에서는 다른 stress에 비해 전체적으로 biofilm형성도가 낮은 값으로 측정되었다. 그리고 6% sodium chlorine solution에서 30-55%의 상해를 입은 Salmonella가 높은 biofilm 형성도를 보였다. 그러나 같은 종의 식중독 세균이라도 외부의 stress 대하여 다양한 정도의 biofilm을 생성하는 것으로 보인다. 따라서 식품으로부터 이들 식중독 세균을 제어하기 위해서는 대상식품의 보존환경에 따른 biofilm 형성특성을 고려해야 할 것으로 사료된다.

• 한국작물학회지
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• 제28권1호
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• pp.76-80
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• 1983

수도의 냉해 경감을 위하여 인산 및 붕소의 시용효과를 구명코저 인산을 10a당 10, 20kg, 붕소를 0, 3, 5, 10, 20, 30kg을 분벽기, 유수형성기 및 감수분열기에 시용하고 유수형성기 이후부터 $15-18^{\circ}C$로 냉수처리한 결과를 요약하면 다음과 같다. 1. 붕소의 시용량이 많아질수록 시용후 3일경부터 염색이 황변하며 생육이 억제되었으며 생육후기에는 염색이 암갈색으로 착색되고 엽선단의 양록이 말리며 고사되었다. 한편 조립의 선단부에도 암갈색으로 착색되었다. 2. 엽록소 함량은 분벽기와 유수형성기에 10a당 5, 10kg의 붕소 시용구에서 높았으며 감수분열기 처리에서는 일정한 경향이 없었다. 3. 광합성능력과 붕소시용간에는 일정한 경향이 없었으나 분벽기 시용구에서는 붕소량이 많을수록 증가되었다. 한편 엽록소 함량이 증가되어도 이에 비례하여 광합성능력은 별로 증대되지 않았다. 4. 근활력은 대체로 무처리구에 비하여 붕소처리구가 다소높았다. 특히 분벽기에 10a당 3, 5, 10kg의 붕소처리구에서 높았다. 5. 붕소처리에 의한 출수촉진효과는 붕소의 시용시기가 빠를수록 촉진되는 경향을 보였고 붕소수준간에는 무처리구에 비하여 붕소를 10a당 3, 5, 10kg 시용구에서 3-7일의 촉진효과를 보였다. 6. 붕소처리는 등숙에 영향을 미쳐 인산수준 및 처리시기에 관계없이 붕소를 10a당 3, 5, 10kg 시용한 구에서 약간 높아졌다.