• Korean Journal of Veterinary Research
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• v.16 no.1
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• pp.7-10
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• 1976

An agglutinability between naturally infected positive chicken serum of pullorum disease and hyperimmumized rabbit antiserum was compared. And the following results were obtained and summarized. 1. On the agglutinability, Salmonella pullorum antigen which irradiated gamma-ray was more excellent than another both formalized and heated antigen. 2. Time of judgemented as positive titer in the tube agglutination test to the naturally infected positive chicken serum was it most suitable for 12 hours at $37^{\circ}C$. 3. Agglutination titer of positive immune chicken serum against gamma-ray irradiate Salmonella pullorum were as 320~640x.

• Korean Journal of Poultry Science
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• v.23 no.3
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• pp.113-119
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• 1996

A series of experiments were conducted to investigate the role of protein kinase C (PKC) as a second messenger in vasoactive intestinal peptide (VIP) mediated prolactin secretion. Primary pituitary cells (106 cells/treatment) were separated from laying hens and incubated in M-199 with 5% chicken serum and 5% fetal calf serum. The VIP(0.1 $\pi$M) treatment enhanced prolactin Secretion into media upto 9-fold during 48-h incubation. The phorbol 12-myristate 13-acetate (PMA), a PKG agonist, increased prolactin secretion upto 2-fold at 0.1 nM PMA (P<0.01), and the prolactin secretion was not significantly higher than this concentration. Staurosporine (ST; 1.0$\pi$M) a PKC antagonist, decreased by 70% of 0.1 $\pi$M VIP-stimulated prolactin secretion and by 48% of 10 ${\mu}$M PMA-stimulated prolactin secretion (P<0.01). However, pituitary cell prolactin content did not differ in any treatment (P>0.05). In conclusion, these results indicate that the PKC second messenger system is involved in VIP-stimulated prolactin release in chicken primary pituitary cell culture.

• Korean Journal of Organic Agriculture
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• v.19 no.4
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• pp.565-575
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• 2011

Recently, the use of antibiotics to improve animal productivity in livestock industry was strictly restricted. For these reason, probiotics have been regarded as one of promising materials for an antibiotic alternative. In this study, we investigated how the probiotics influences on the performance of broiler chicken via meta-analysis. Eighteen researches from 1997 to 2010 were used for meta-analysis. The standard summary effects were calculated via fixed effect model and random effect model (Borenstein et al., 2009). Heterogeneity was calculated by using the Cochran's Q statistics (Kook et al., 2009) and publication bias was calculated via Egger's regression (Lee et al., 2011). In fixed model average daily gain, body weight serum protein content and cecal LAB showed positive effect significantly. Feed intake, feed/gain and serum cholesterol showed significant negative effect. In serum triglyceride, negative effect was found but significance was not shown. In random model, average daily gain body weight and cecal LAB showed positive effects with significance and feed/gain and serum cholesterol represented significant negative effects. Publication bias was found only in feed/gain.

• Korean Journal of Veterinary Research
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• v.29 no.4
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• pp.493-501
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• 1989

A total of 108 strains of C jejuni isolated from pigs and chickens were examined for the susceptibility to 10 antimicrobial agents and normal sera of cattle, sheep, guinea pigs and chickens. Minimal inhibitory concentration(MIC) ranges of antimicrobial agents to C jejuni isolates were $${\leq_-}1.56$$ to $${\geq_-}100{\mu}g/ml$$ for erythromycin, rifampin, streptomycin and tetracycline, 50 to $${\geq_-}100{\mu}g$$ for cephalothin, $${\leq_-}1.56$$ to $50{\mu}g$ for ampicillin, $${\leq_-}1.56$$ to $25{\mu}g$ for kanamycin and nalidixic acid, $${\leq_-}1.56$$ to $12.5{\mu}g$ for chloramphenicol and gentamicin. Resistance rates of C jejuni were showed to in order of rifampin(84.7%), tetracycline(56.2 %), erythromycin(17.1%) and ampicillin(3.8%), all of the strains were sensitive to chloramphenicol, gentamicin and kanamycin, and the incidence rates of resistant C jejuni were highly frequent in pig isolates than chicken isolates. The drug resistance patterns of 87 chicken isolates C jejuni to 9 antimicrobial drugs were showed 12 patterns, and Sm Ra Tc(24.1%), Sm Ra(21.8%) and Ra Tc(14.9%) were relatively common, and also 21 pig isolates were showed 6 patterns and Em Sm Ra Tc(57.1%) were most frequent. The majority of the isolates showed multiple drug resistance. Bactericidal activity of 10% normal sera from healthy animals were examined for 60min at $37^{\circ}C$. C jejuni were decreased from 0.4 to 1.0 ${\log}_{10}$(p<0.01), and serum susceptibility were high in order of guinea pig, sheep, chicken and cattle sera. Serum sensitivity of C jejuni Ch-39 strain in increased serum concentation up to 10, 20, 40 and 80% were highly significant. In the normal animal serum, the number of Ch-39 strain were decreased from $1.8{\times}10^4/ml$ to $2.7{\times}10^3/ml$ after 60 min incubation(p<0.01), but the numbers were decreased to $6.6{\times}10^3/ml$ in the heat inactivated normal serum for 30 min at $56^{\circ}C$. Bactericidal activity was restored in the heat inactivated normal serum after the serum of complement source was added.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.193-199
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• 2010

We report here the production of transgenic chickens that can regulate human erythropoietin (hEPO) gene expression. The glycoprotein hormone hEPO is an essential for viability and growth of the erythrocytic progenitors. Retrovirus vector system used in this study has two features including tetracycline-controllable promoter and woodchuck hepatitis virus posttranscriptional regulator element (WPRE). The former is for to reduce the possibility of physiological disturbance due to constitutional and unregulated expression of hEPO gene in the transgenic chicken. The latter is for maximum expression of the foreign gene when we turn-on the gene expression. A replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of non-incubated chicken embryos (stage X). Out of 325 injected eggs, 28 chicks hatched after 21 days of incubation and 16 hatched chicks were found to express the hEPO gene delivered by the vector. The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially available counterpart. The recombinant hEPO in transgenic chicken serum had N- and O-linked carbohydrate simillar to that produced from in vitro cultured cells transformed with hEPO gene.

• Korean Journal of Veterinary Service
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• v.16 no.2
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• pp.120-126
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• 1993

Clinically healthy 66 female Maniker breed chicken(22 of 2-week-old : group A, 23 of 8-week-old： group B and 21 of 27-week-old： group C) and 66 female Manina breed chicken (21 of 2-week-old : group D, 22 of 8-week-old : group E and 23 of 27-week-old : group F) were examined to establish physiological basic data on serum total Creatine phosphokinase (CPK) activities and CPK isoenzymes fractions. The results obtained were summarized as follows； 1. Serum total CPK activities were $1,088{\pm}254.0\;IU\;/{\ell},$ $1,454{\pm}337.2\;IU\;/{\ell}$ and $1,440{\pm}526.3\;IU\;/{\ell}$ in gorup A, group B and group C of Maniker breed, respectively. Group B and group C showed higher values than that of group A (P<0.01) meaning high values nth aging. 2. Serum total CPK activities were $1,676{\pm}420.5\;IU\;/ {\ell},$ $1,007{\pm}283.1\;IU\;/{\ell}$ and $862{\pm}294.5\;IU/{\ell}$ in group D, group E and group F of Manina breed, respectively. Group D showed the highest value among groups (P<0.01) and Manina breed showed the low values of serum total CPK activities with aging. 3. Manina breed at 2-week-old and Maniker breed at 8-week-old and 27-week-old showed significant high values of total serum CPK activities in breed differance (P<0.01) 4. In the pattern of serum CPK isoenzyme fractions, group A and group B were high with decreasing order of $CK_3>CK_2>CK_1$ and group C was high with decreasing order of $CK_2> CK_3>CK_1$ Groihp D, E and F showed the same pattern with decreasing order of $CK_2>CK_3> CK_1.$ 5. Significance of CPK isoenzymes fractions in breed differance was only found at 8-week-old. $CK_1\;and \;CK_3$ in Maniker breed (P<0.05), and $CK_2$ in Manina breed were higher than that of the other breed (P < 0.01).

• Animal Bioscience
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• v.34 no.4
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• pp.670-679
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• 2021

Objective: Glucose transporter 9 (GLUT9) is a uric acid transporter that is associated with uric absorption in mice and humans; but it is unknown whether GLUT9 involves in chicken uric acid regulation. This experiment aimed to investigate the chicken GLUT9 expression and serum uric acid (SUA) level. Methods: Sixty chickens were divided into 4 groups (n = 15): a control group (NC); a sulfonamide-treated group (SD) supplemented with sulfamonomethoxine sodium via drinking water (8 mg/L); a fishmeal group (FM) supplemented with 16% fishmeal in diet; and a uric acid-injection group (IU), where uric acid (250 mg/kg) was intraperitoneally injected once a day. The serum was collected weekly to detect the SUA level. Liver, kidney, jejunum, and ileum tissues were collected to detect the GLUT9 mRNA and protein expression. Results: The results showed in the SD and IU groups, the SUA level increased and GLUT9 expression increased in the liver, but decreased in the kidney, jejunum, and ileum. In the FM group, the SUA level decreased slightly and GLUT9 expression increased in the kidney, but decreased in the liver, jejunum, and ileum. Correlation analysis revealed that liver GLUT9 expression correlated positively, and renal GLUT9 expression correlated negatively with the SUA level. Conclusion: These results demonstrate that there may be a feedback regulation of GLUT9 in the chicken liver and kidney to maintain the SUA balance; however, the underlying mechanism needs to be investigated in future studies.

• Journal of radiological science and technology
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• v.9 no.1
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• pp.137-143
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• 1986

This study was undertaken to analyze the changes of radiographical image in avian (chicken) by using scanner and ${\gamma}-camera$ with $Tc^{99m}-MDP$. Experiment was aimed also to observe the changes of calcium, phosphorus and alkaline phosphatase activity in serum after experimental fracture. The results obtained are summerized as follows. 1. 10 chickens grown 6 weeks were scanned within 48 hours after experimental fracture, showed localized increase in uptake. The earlist increased local uptake was observed after 7 hours from experimental fracture at the fracture site. 2. Serum alkaline phosphatase level was increasing continuously during the fracture healing process in comparison with control group and serum alkaline phosphatase activity showed a peak after 2 weeks following the fracture. 3. The change of Ca. amount in serum trended to decrease from 1st day after fracture and it was in the lowest level at the 7 day's group following the fracture. 4. Serum phosphorus level trended to increase after fracture and it was in the highest level at the 7 day's group and showed similar level in comparison with control group.

• Korean Journal of Veterinary Research
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• v.29 no.4
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• pp.503-515
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• 1989

Critical parameters affecting sensitivity and specificity of an enzyme-linked immunosorbent assay(ELISA) for detection of antibodies to avian infections bronchitis virus(IBV) were standardized. By adopting the optimized conditions an equation calculating ELISA antibody titers from the observations at single serum dilution was formulated. The purified antigen of IBV Mass-41 strain was dispensed into polystyrene microplate wells at a concentration of 300ng per well($100{\mu}l$) and the plates were coated by completey drying at $37^{\circ}C$. Diluted chicken serum and horseradish peroxidase conjugated goat anti-chicken IgG were added in order in $100{\mu}l$ volumes per well and allowed to react for 30 minutes each at room temperature. Just before use and after each reaction the plates were washed three times with distilled water. Finally o-phenylenediamine solution was added as an enzyme substrate. After incubation for another 15 minutes at room temperature absorbances were read at 492nm. Hyperimmune serum against Mass-41 strain was used as internal reference positive(IRP) serum. After repeated titration of IRP and negative serum, a constant titer of IRP was determined. Serum titrations were carried out for various sample sera together with IRP and negative sera and the observed titers of sample sera were corrected by reflecting the ratio between observed and constant titers of IRP serum. These corrected titers of the sample sera were plotted against sample/positive(S/P) OD ratios. All the OD's measured in the serum titrations were also corrected by substracting negative serum OD. The following equation was formulated from the above data; $Log_{10}$ ELISA titer=$5.568({\log}_{10}S/P)+4.161$ Thus it was possible to calculate ELISA titer by measuring absorbance at 1/400 single serum dilution. Titer measured by cross ELISA tests employing Mass-41 strain and three local IBV isolates were similar. These results suggest that the ELISA tests standardized in this study can be used for evaluating not only vaccinal immunity but also for infection status against fields IBV's.

• Korean Journal of Veterinary Research
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• v.45 no.4
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• pp.569-574
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• 2005

Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to reticuloendotheliosis virus (REV) at single serum dilution was standardized. REV HI, one of the Korean field isolates, was inoculated into chicken embryo fibroblast (CEF) cells and was harvested from the culture fluids and cells after 10 to 12 days. Viruses were purified by centrifugation at the $107,000{\times}g$ for 12 hours on 20, 30, 45% (W/V) sucrose gradient. Virus specific fraction was collected and used as ELISA antigen. To standardize ELISA, the optimal concentration of coating antigen ($1{\mu}g/well$) and conjugate (1/1000) was determined by corrected OD (OD value of positive serum-OD value of negative serum) and P/N ratio (OD value of positive serum/OD value of negative serum). To calculate ELISA titer by measuring absorbance at 1/400 single serum dilution, serum titrations were carried out for various sample sera together with standard positive and negative sera. The observed titers of serum samples were plotted against sample/positive (s/p) ratios at 1/400 serum dilution. From the above data, the ELISA titers could be calculated by the equation of $log_{10}$ ELISA titer = 2.2763 ($log_{10}$ s/p) + 3.482 (r = 0.93). For evaluating the sensitivity, the standardized method were compared with conventional agar gel immunodiffusion (AGID) test method using serum samples collected from REV infected field chicken flocks. Fifty seven of 60 samples (95%) were positive for REV by ELISA, whereas only 11 (18.3%) samples were positive by AGID test. This results suggested that the ELISA tests developed in this study could be used for detection of antibodies to REV with high sensitivity.