Reproductive and Developmental Biology

  • 제34권3호
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  • Pages.193-199
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  • 2010
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

hEPO 유전자의 발현이 조절되는 형질전환 닭의 생산

Generation of Transgenic Chickens Regulating hEPO Gene Expression

  • 구본철 (대구가톨릭대학교 의과대학 생리학교실) ;
  • 권모선 (대구가톨릭대학교 의과대학 생리학교실) ;
  • 김태완 (대구가톨릭대학교 의과대학 생리학교실)
  • Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kwon, Mo-Sun (Department of Physiology, Catholic University of Daegu School of Medicine) ;
  • Kim, Te-Oan (Department of Physiology, Catholic University of Daegu School of Medicine)
  • 투고 : 2010.09.08
  • 심사 : 2010.09.14
  • 발행 : 2010.09.30
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초록

We report here the production of transgenic chickens that can regulate human erythropoietin (hEPO) gene expression. The glycoprotein hormone hEPO is an essential for viability and growth of the erythrocytic progenitors. Retrovirus vector system used in this study has two features including tetracycline-controllable promoter and woodchuck hepatitis virus posttranscriptional regulator element (WPRE). The former is for to reduce the possibility of physiological disturbance due to constitutional and unregulated expression of hEPO gene in the transgenic chicken. The latter is for maximum expression of the foreign gene when we turn-on the gene expression. A replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of non-incubated chicken embryos (stage X). Out of 325 injected eggs, 28 chicks hatched after 21 days of incubation and 16 hatched chicks were found to express the hEPO gene delivered by the vector. The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially available counterpart. The recombinant hEPO in transgenic chicken serum had N- and O-linked carbohydrate simillar to that produced from in vitro cultured cells transformed with hEPO gene.

키워드

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