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Generation of Transgenic Chickens Regulating hEPO Gene Expression  

Koo, Bon-Chul (Department of Physiology, Catholic University of Daegu School of Medicine)
Kwon, Mo-Sun (Department of Physiology, Catholic University of Daegu School of Medicine)
Kim, Te-Oan (Department of Physiology, Catholic University of Daegu School of Medicine)
Publication Information
Reproductive and Developmental Biology / v.34, no.3, 2010 , pp. 193-199 More about this Journal
Abstract
We report here the production of transgenic chickens that can regulate human erythropoietin (hEPO) gene expression. The glycoprotein hormone hEPO is an essential for viability and growth of the erythrocytic progenitors. Retrovirus vector system used in this study has two features including tetracycline-controllable promoter and woodchuck hepatitis virus posttranscriptional regulator element (WPRE). The former is for to reduce the possibility of physiological disturbance due to constitutional and unregulated expression of hEPO gene in the transgenic chicken. The latter is for maximum expression of the foreign gene when we turn-on the gene expression. A replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of non-incubated chicken embryos (stage X). Out of 325 injected eggs, 28 chicks hatched after 21 days of incubation and 16 hatched chicks were found to express the hEPO gene delivered by the vector. The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially available counterpart. The recombinant hEPO in transgenic chicken serum had N- and O-linked carbohydrate simillar to that produced from in vitro cultured cells transformed with hEPO gene.
Keywords
Transgenic chicken; Human erythropoietin (hEPO); Tet system; Retrovirus vector;
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1 Motono M, Yamada Y, Hattori Y, Nakagawa R, Nishijima K-i, Iilima S (2010): Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector. J Biosci Bioeng 109: 315-321.   DOI   ScienceOn
2 Dyck MK. Lacroix D, Pothier F, Sirard MA (2003): Making recombinant proteins in animals-different systems, different applications. Trends Biotechnol 21:394-399.   DOI   ScienceOn
3 Harvey AJ, Speksnijder G, Baugh LR, Morris JA, Ivarie R (2002): Expression of exogenous protein in the egg white of transgenic chickens. Nat Biotechnol 20:396-399.   DOI   ScienceOn
4 Kwon MS, Koo BC, Choi BR, Lee HT, Kim YH, Ryu WS, Shim H, Kim JH, Kim NH, Kim T (2004): Development of transgenic chickens expressing enhanced green fluorescent protein. Biochem Biophys Res Commun 320:442-448.   DOI   ScienceOn
5 Whitelaw CB (2004): Transgenic livestock made easy. Trends Biotechnol 22:157-159.   DOI   ScienceOn
6 Rush RS, Derby PL, Smith DM, Merry C, Rogers G, Rohde MF, Katta V (1995): Microheterogeneity of erythropoietin carbohydrate structure. Anal Chem 67:1442-1452.   DOI   ScienceOn
7 Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, Hillen W (2000): Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA 97:7963-7968.   DOI   ScienceOn
8 van de Lavoir MC, Diamond JH, Leighton PA, Mather-Love CM, Heyer BS, Bradshaw R, Kerchner A, Hooi LT, Gessaro TM, Swanberg SE, Delany ME, Etches RJ (2006): Germline transmission of genetically modified primordial germ cells. Nature 441:766-769.   DOI   ScienceOn
9 Zufferey R, Donello JE, Trono D, Hope TJ (1999): Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J Virol 73:2886-2892.
10 Zhu L, van de Lavoir MC, Albanese J, Beenhouwer DO, Cardarelli PM, Cuison S, Deng DF, Deshpande S, Diamond JH, Green L, Halk EL, Heyer BS, Kay RM, Kerchner A, Leighton PA, Mather-Love CM, Morrison SL, Nikolov ZL, Passmore DB, Pradas-Monne A, Preston BT, Rangan VS, Shi M, Srinivasan M, White SG, Winters-Digiacinto P, Wong S, Zhou W, Etches RJ (2005): Production of human monoclonal antibody in eggs of chimeric chickens. Nat Biotechnol 23:1159-1169.   DOI   ScienceOn
11 McGrew MJ, Sherman A, Ellard FM, Lillico SG, Gilhooley HJ, Kingsman AJ, Mitrophanous KA, Sang H (2004): Efficient production of germline transgenic chickens using lentiviral vectors. EMBO Rep 5:728-733.   DOI   ScienceOn
12 Park JK, Lee YK, Lee P, Chung HJ, Kim S, Lee HG, Seo MK, Han JH, Park CG, Kim HT, Kim YK, Min KS, Kim JH, Lee HT, Chang WK (2006): Recombinant human erythropoietin produced in milk of transgenic pigs. J Biotechnol 122:362-371.   DOI   ScienceOn
13 Park TS, Jeong DK, Kim JN, Song GH, Hong YH, Lim JM, Han JY (2003): Improved germline transmission in chicken chimeras produced by transplantation of gonadal primordial germ cells into recipient embryos. Biol Reprod 68:1657-1662.
14 Rapp JC, Harvey AJ, Speksnijder GL, Hu W, Ivarie R (2003): Biologically active human interferon $\alpha$-2b produced in the egg white of transgenic hens. Transgenic Res 12:569-575.   DOI   ScienceOn
15 Penno CA, Kawabe Y, Ito A, Kamihira M (2010): Production of recombinant human erythropoietin/FC fusion protein by genetically manipulated chickens. Transgenic Res 19:187-195.   DOI   ScienceOn
16 Petitte JN, Mozdziak PE (2002): Transgenic chickens as bioreactors for protein-based drugs. In Pinkert CA, editor. Transgenic Animal Technology, 2nd edn. San Diego: Academic Press. p279-306.
17 Raju TS, Briggs JB, Borge SM, Jones AJ (2000): Species-specific variation in glycosylation of IgG: evidence for the species-specific sialylation and branch-specific galactosylation and importance for engineering recombinant glycoprotein therapeutics. Glycobiology 10:477-486.   DOI   ScienceOn
18 Kim T (2002): Transgenic chickens as bioreactors for protein-based drugs. In: Pinkert CA (ed) Transgenic Animal Technology, 2nd edn. Academic Press, San Diego, pp 173-193.
19 Kitamura T, Tnage T, Terasawa T, Chiba S, Kuwaki T, Miyagawa K, Piao Y-F, Miyazono K, Urabe A, Takaru F (1989): Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3 or erythropoietin. J Cell Physiol 140:323-334.   DOI
20 Kodama D, Nishimiya D, Iwata K, Yamaguchi K, Yoshida K, Kawabe Y, Motono M, Watanabe H, Yamashita T, Nishijima K, Kamihira M, Iijima S (2008): Production of human erythropoietin by chimeric chickens. Biochem Biophys Res Commun 367:834-839.   DOI   ScienceOn
21 Elliott S, Egrie J, Browne J, Lorenzini T, Busse L, Rogers N, Ponting I (2004): Control of rHuEPO biological activity: the role of carbohydrate. Exp Hematol 32:1146-1155.   DOI   ScienceOn
22 Koo BC, Kwon MS, Choi BR, Kim JH, Cho SK, Sohn SH, Cho EJ, Lee HT, Chang W, Jeon I, Park JK, Park JB, Kim T (2006): Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector. FASEB J 20:2251-2260.   DOI   ScienceOn
23 Kwon MS, Koo BC, Choi BR, Kim JH, Park YY, Lee YM, Suh HS, Park YS, Lee HT, Kim JH, Roh JY, Kim NH, Kim T (2008): Generation of transgenic chickens that produce bioactive human granulocyte-colony stimulating factor. Mol Reprod Dev 75:1120-1126.   DOI   ScienceOn
24 Lillico SG, McGrew MJ, Sherman A, Sang HM (2005): Transgenic chickens as bioreactors for protein-based drugs. Drug Discov Today 10:191-196.   DOI   ScienceOn
25 Eyal-Giladi H, Kochav S (1976): From cleavage to primitive streak formation: a complementary normal table and a new look at the first stages of the development of the chick. Dev Biol 49:321-337.   DOI   ScienceOn
26 Gossen M, Bujard H (1992): Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89:5547-5551.   DOI   ScienceOn
27 Kamihira M, Ono KI, Esaka K, Nishijima KI, Kigaku R. Komatsu H, Yamashita T, Kyogoku K, lijima S (2005): High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector. J Virol 79:10864-10874.   DOI   ScienceOn
28 Gossen M, Freundlieb S, Bender G, Miiller G, Hillen W, Bujard H (1995): Transcriptional activation by tetracyclines in mammalian cells. Science 268:1766-1769.   DOI
29 Ivarie R (2003): Avian transgenesis: progress towards the promise. Trends Biotechnol 21:14-19.   DOI   ScienceOn
30 Jelkmann W (2004): Molecular biology of erythropoietin. Intern Med 43:649-659.   DOI   ScienceOn
31 Burns JC, Friedmann T, Driever W, Burrascano M, Yee JK (1993): Vesicular stomatitis virus G glycoprotein pseudo typed retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells. Proc Natl Acad Sci USA 90: 8033-8037.   DOI   ScienceOn
32 Aguirre A Castro-Palomino N, de la Fuente J, Castro FO (1998): Expression of human erythropoietin transgenes and of the endogenous wap gene in the mammary gland of transgenic rabbits during gestation and lactation. Transgenic Res 7:311-317.   DOI   ScienceOn