The Role of Protein Kinase C for Prolactin Secretion in Chicken Primary Pituitary Cell Culture

산란계의 뇌하수체 세포배양에서 Prolactin의 생성에 관계하는 Protein Kinase C의 역할

  • 선상수 (전남대학교 동물자원학과)
  • Published : 1996.09.01

Abstract

A series of experiments were conducted to investigate the role of protein kinase C (PKC) as a second messenger in vasoactive intestinal peptide (VIP) mediated prolactin secretion. Primary pituitary cells (106 cells/treatment) were separated from laying hens and incubated in M-199 with 5% chicken serum and 5% fetal calf serum. The VIP(0.1 $\pi$M) treatment enhanced prolactin Secretion into media upto 9-fold during 48-h incubation. The phorbol 12-myristate 13-acetate (PMA), a PKG agonist, increased prolactin secretion upto 2-fold at 0.1 nM PMA (P<0.01), and the prolactin secretion was not significantly higher than this concentration. Staurosporine (ST; 1.0$\pi$M) a PKC antagonist, decreased by 70% of 0.1 $\pi$M VIP-stimulated prolactin secretion and by 48% of 10 ${\mu}$M PMA-stimulated prolactin secretion (P<0.01). However, pituitary cell prolactin content did not differ in any treatment (P>0.05). In conclusion, these results indicate that the PKC second messenger system is involved in VIP-stimulated prolactin release in chicken primary pituitary cell culture.

Keywords

References

  1. Endocrinol v.119 Vasoactive intestinal polypeptide is synthesized anterior pituitary tissue Arnaout,M.A.;Garthwaite,T.L.;Martinson,D.R.;Hagen,T.C.
  2. Ann Rev Biochem v.56 Inositol triphosphate and diacylglycerol: two interacting second messengers Berridge,M.J.
  3. Endocrinol v.132 Protein kinase Cregulation of prolactin gene expression lactotroph cells: involvement in dopamine inhibition Chuang,T.T.;Caccavelli,L.;Kordon,C.;Enjalbert,A.
  4. Gen Comp Endocrinol v.80 Enhanced vasoactive intestinal peptideinduced prolactin secretion from anterior pituitary cells of incubating turkeys (Meleagris gallopavo) EI Halawani,M.E.;Silsby,J.L.;Mauro,L.J.
  5. Sc v.199 Phosphorylated proteins as physiological effectors Greengard,P.
  6. Neuroendoc v.43 Antiserato vasoactive intestinal polypeptide inhibit basal prolactin release from dispersed anterior pituitary cells Hagen,T.C.;Arnaout,M.A.;Scherzer,W.J.;Martinson,D.R.;Garthwaite,T.L.
  7. J Cell Biol v.59 Hormone secretion by cells dissociated from rat anterior pituitaries Hopkins,C.R.;Farquhar,M.G.
  8. PRL Gene Family and Its Receptors Systems that regulate prolactin release MacLeod,R.M.;Judd,A.M.;Spangelo,B.L.;Ross,P.C.;Jarvis,W.D.;Login,IS.;K.Hoshino(ed.)
  9. J Biol Chem v.264 Both isoforms of the cAMP-de-pendent protein kinase catalytic subunit can activate transcription of the prolactin gene Maurer,R.A.
  10. Prolactin secretion and its control. In: The Physiology of Reproduction(2nd ed.) Neill,J.D.;Nagy,G.M.;E.Knobil.;J.D.Neill(ed.)
  11. J Biochem v.115 Vasoactive intestinal peptide induces differentiation and MAP kinase activation in PC12h cells Okumura,N.;Miyatake,Y.;Takao,T.;Tamaru,T.;Nagai,K.;Okada,M.;Nakagawa,H.
  12. Biol Reprod v.50 Role of vasoactive intestinal peptide in the control of prolactininduced turkey incubation behavior.Ⅰ.Acute infusion of vasoactive intestinal peptide Pitts,G.R.;Youngren,O.M.;Silsby,J.L.;Foster,L.K.;Foster,D.N.;Rozenboim,I.;Phillips,R.E.;EI Halawani,M.E.
  13. Biol Reprod v.25 Turkey prolactin: Validation of a radioimmunoassay and measurement of changes associated with broodiness Proudman,J.A.;Opel,H.
  14. Scand J Gastroenterol v.29 Cyclic adenosine monophosphate is the second messenger of prostaglandin E₂and vasoactive intestinal polypeptide-stimulated active bicarbonate secretion by guinea-pig duodenum Reimer,R.;Odes,H.S.;Muallem,R.;Schwenk,M.;Beil,W.;Sewing,K.F.R.
  15. Mol Neurobiol v.5 The role of protein kinase C and its neuronal substrates dephosphin, B-50, and MARKCKS in neurotransmitter release Robinson,P.J.
  16. SAS User's Guide: Statistics(5th ed.) SAS Institute
  17. Endocrinol v.108 Immunoreactive vasoactive intestinal polypeptide in rat hypophysial portal blood Shimatsu,A.;Kato,Y.;Matsushita,N.;Katakami,H.;Yanaihara,N.;Imura,H.
  18. Vitamins and Hormones v.43 The hormonal regulation of prolactin gene expression: an examination of mechanisms controlling prolactin synthesis and the possible relationship of estrogen to these mechanisms Shull,J.D.;Gorski,J.
  19. Endocrinol v.131 Hypothyroidism increases vasoactive intestinal polypeptide (VIP) immunoreactivity and gene expression in the rat hypothalamic paraventricular nucleus Toni,R.;Kakucska,I.;Mosca,S.;Marrama,P.;Lechan,R.M.