• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.761-762
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• 2003

Recently, several studies have reported on the successful repair of osteochondral defects by transplantation of cultured chondrocytes, but the method requires a sufficient number of cells obtained from the donor site in the articular cartilage. This can potentially be overcome by the use of undifferentiated or partially developed cartilage precursor cells drived from early embryos and fetal tissue. Neonatal cartilage unlike adult cartilage has the capacity for rapid regeneration. the purpose of this study is to determine effective regeneration method using early cartilage precursors for tissue-engineered cartilage. Cells isolated from neonatal (immediately postpartum, 2 hours of age) SD rats were seeded onto biodegradable polymer matrices and transplanted in nude mice's subcutaneous sites for 4 and 8 weeks. Tissue-engineered cartilage showed gross and histologic evidences similar to native articular cartilage.

• Archives of Craniofacial Surgery
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• v.19 no.1
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• pp.79-82
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• 2018

Formation of an ideally-shaped tragus remains one of the most challenging issues during staged tragus reconstruction in microtia patients. The authors describe a new method used to treat a unique case of concha-type microtia in which the 10-year-old male patient had only a portion of pre-existing cartilage at the tragus site. An anomalous skin lump was also present. During the initial stages of the reconstruction, the two-stage Nagata method was used for surgical correction of the microtia. An autologous rib cartilage graft was used to form the ear framework. A temporoparietal fascia flap was also constructed. Remnant skin tags and anomalous cartilage that accompany microtia are usually removed during microtia repair. However, the cartilage and skin lump were preserved during the reconstruction. The skin lump was later used to form a vascularized chondrocutaneous island flap that supplemented the portion of cartilage during tragus formation. The result was a new tragus that was satisfactorily improved in both size and shape. Patients with concha-type microtia may benefit from the use of this new method for tragus formation.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.48-50
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• 2002

Cartilage defects are common and painful conditions that affect people of all ages. Although many techniques have developed, none of the current available treatment options is satisfactory. Recent advances in biology and materials science have pushed tissue engineering to the forefront of new cartilage repair techniques. The purpose of this study is to determine effective regeneration method for tissue-engineered cartilage. A serum free medium was developed for cartilage tissue engineering. Chondrocyte passage number was found to influence greatly on cartilage tissue formation in vivo. Injectable, biodegradable polymer matrix was developed for chondrocyte transplantation through injection. Transplantation of chondrocytes mixed with the injectable matrices resulted in the cartilage formation in nude mice's subcutaneous sites and rabbit knees. This study may lead to the development of tissue-engineered cartilage appropriate for clinical applications.

• 대한관절경학회:학술대회논문집
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• 2008.04a
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• pp.88-89
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• 2008

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.364-367
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• 2006

In order to enhance the repair of defects in articular cartilage via cell therapy with autologous chondrocytes, as well as with periosteum-derived progenitor cells (PDPCs), silkworm hemolymph (SH) and a variety of cartilage-specific extracellular matrices (ECMs) including type II collagen, proline, chondroitin 4-sulfate, and chondroitin 6-sulfate were assessed with regard to their efficacy as media supplements. SH, a known anti-apoptotic agent, was found to enhance cell growth, as was shown by the results of a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to the results of reverse transcriptase polymerase chain reaction (RT-PCR) analyses, the cartilage-specific ECMs were found to stimulate the expression of hyaline cartilage-specific genes, most notably type II collagen and Sox9, in monolayer cultures of PDPCs.

• Journal of Trauma and Injury
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• v.23 no.1
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• pp.53-55
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• 2010

A thyroid cartilage fracture is a rare entity and can be overlooked easily. Such cases are difficult to diagnose, and assessment and treatment guidelines are difficult to determine. CT of the neck region may be useful when acute airway intervention is not required or when more information regarding the neck's anatomy is required for management decisions. We describe a case of a thyroid cartilage fracture with displacement. In the emergency department (ED), neck CT and fiberoptic nasopharyngoscopy were used to assess the status of the patient's (a male) vocal chords immediately. He remained unable to phonate continuously. After an immediate assessment, we decided to use steroid and conservative therapy. The patient had a good recovery and was without symptoms one month after injury. There is no question that early surgical repair of neck injuries affords the best results for airway and voice patency in most cases however, we suspect that surgical repair is not needed in all cases. Early recognition and an accurate therapy plan for a thyroid fracture with displacement are essential. Therefore, the emergency physician's immediate and careful decision based on endoscopy and neck CT is important for the patient's long-term recovery.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.618-622
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• 2005

Articular cartilage is an important target for studying the arthritic diseases. To verify the therapeutic effects of bee venom herb-acupuncture in vivo, 3${\mu}$l of diluted solution of bee venom for herb-acupuncture were injected into articular cavity once a day during 3 months after making full-thickness defects in rat articular cartilage. Histological examination and immunohistochemistry indicated that the chondrocyte-like tissue was formed during the repair process of cartilage injury, and the expression of a cartilage-specific protein, collagen type II, were significantly activated. It means that the expression of the gene encoding type I collagen was down-regulated, whereas those of collagen type II were up-regulated. Histological examination by hematoxylin-eosin staining indicated that the cells regained their original round morphology. In addition, a homogeneous distribution of articular cartilage extracellular matrices was detected around the cells. These results suggested that bee venom herb-acupuncture was very effective on the recovery of articular chondrocyte phenotype.

• Investigative Magnetic Resonance Imaging
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• v.21 no.3
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• pp.187-191
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• 2017

Bone marrow aspirates concentrate (BMAC) transplantation is a well-known technique for cartilage regeneration with good clinical outcomes for symptoms in patients with osteoarthritis (OA). Magnetic resonance imaging (MRI) has an important role in evaluating the degree of cartilage repair in cartilage regeneration therapy instead of a second assessment via an arthroscopy. We experienced a case of hypertrophic regeneration of the cartilage and a presumed simultaneous regeneration of the posterior horn of the lateral meniscus after BMAC transplantation for a cartilage defect at the lateral tibial and femoral condyle. This report provides the details of a case of an unusual treatment response after a BMAC transplant. This report is the first of its kind to demonstrate a MR image that displays the simultaneous regeneration of the cartilage and meniscus with a differentiation ability of the mesenchymal stem cell to the desired cell lineage.

• 대한정형외과스포츠의학회:학술대회논문집
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• 2005.04a
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• pp.51-51
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• 2005

• Maxillofacial Plastic and Reconstructive Surgery
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• v.17 no.2
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• pp.153-158
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• 1995

A wide variety of deformities can occur following repair of the cleft lip. Especially, cleft lip nasal deformities offer the severe psychologic, esthetic, and functional impairment. We must restore the deformities of alar cartilge, nasal tip, septum, columella, or pyriform aperture. The authors reconstructed the cleft lip nasal deformities using with the alar cartilage rearrangement, postauricular cartilage graft, and/or columellar lengthening. The 3 case reports are presented.