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Effect of Bee Venom Herb-acupuncture on the Repair of Articular Full-thickness Defect in Rat  

Zhao, Mei-Ai (Department of Oriental Medical Science, Graduate School of East-West Medial Science, Kyung-Hee University)
Hahm, Dae-Hyun (Department of Oriental Medical Science, Graduate School of East-West Medial Science, Kyung-Hee University)
Lee, Seung-Ki (Ilchim Oriental Hospital)
Choi, Sun-Mi (Department of Medical research, Korea Institute of Oriental Medicine)
Kim, Gun-Ho (Department of Oriental Medical Science, Graduate School of East-West Medial Science, Kyung-Hee University)
Shim, In-Sop (Department of Integrative Medicine, College of Medicine, The Catholic University of Korea)
Kang, Sung-Keel (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University)
Lee, Hye-Jung (Department of Oriental Medical Science, Graduate School of East-West Medial Science, Kyung-Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.3, 2005 , pp. 618-622 More about this Journal
Abstract
Articular cartilage is an important target for studying the arthritic diseases. To verify the therapeutic effects of bee venom herb-acupuncture in vivo, 3${\mu}$l of diluted solution of bee venom for herb-acupuncture were injected into articular cavity once a day during 3 months after making full-thickness defects in rat articular cartilage. Histological examination and immunohistochemistry indicated that the chondrocyte-like tissue was formed during the repair process of cartilage injury, and the expression of a cartilage-specific protein, collagen type II, were significantly activated. It means that the expression of the gene encoding type I collagen was down-regulated, whereas those of collagen type II were up-regulated. Histological examination by hematoxylin-eosin staining indicated that the cells regained their original round morphology. In addition, a homogeneous distribution of articular cartilage extracellular matrices was detected around the cells. These results suggested that bee venom herb-acupuncture was very effective on the recovery of articular chondrocyte phenotype.
Keywords
articular cartilage; injury; collagen type II; bee venom; herb-acupuncture;
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