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Effects of Silkworm Hemolymph and Cartilage-specific Extracellular Matrices on Chondrocytes and Periosteum-derived Progenitor Cells  

Shin, Hyun-Chong (Department of Biological Engineering, Inha University)
Choi, Yong-Soo (Department of Biological Engineering, Inha University)
Lim, Sang-Min (Department of Biological Engineering, Inha University)
Lee, Chang-Woo (Good Shepherd Hospital)
Kim, Dong-Il (Department of Biological Engineering, Inha University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.11, no.4, 2006 , pp. 364-367 More about this Journal
Abstract
In order to enhance the repair of defects in articular cartilage via cell therapy with autologous chondrocytes, as well as with periosteum-derived progenitor cells (PDPCs), silkworm hemolymph (SH) and a variety of cartilage-specific extracellular matrices (ECMs) including type II collagen, proline, chondroitin 4-sulfate, and chondroitin 6-sulfate were assessed with regard to their efficacy as media supplements. SH, a known anti-apoptotic agent, was found to enhance cell growth, as was shown by the results of a 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to the results of reverse transcriptase polymerase chain reaction (RT-PCR) analyses, the cartilage-specific ECMs were found to stimulate the expression of hyaline cartilage-specific genes, most notably type II collagen and Sox9, in monolayer cultures of PDPCs.
Keywords
chondrocytes; periosteum-derived progenitor; silkworm hemolymph; extracellular matrix; cartilage;
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1 Hunziker, E. B. (2000) Articular cartilage repair: Problems and perspectives. Biorheology 37: 163-164
2 Evans, C. H. and H. I. Georgescu (1983) Observations on the senescence of cells derived from articular cartilage. Mech. Ageing Dev. 22: 179-191   DOI   ScienceOn
3 Choi, S. S., W. J. Rhee, and T. H. Park (2002) Inhibition of human cell apoptosis by silkworm hemolymph. Biotechnol. Prog. 18: 874-878   DOI   ScienceOn
4 Freed, L. E., A. P. Hollander, I. Martin, J. R. Barry, R. Langer, and G. Vunjak-Novakovic (1998) Chondrogenesis in a cell-polymer-bioreactor system. Exp. Cell Res. 240: 58-65   DOI   ScienceOn
5 Malpeli, M., N. Randazzo, R. Cancedda, and B. Dozin (2004) Serum-free growth medium sustains commitment of human articular chondrocyte through maintenance of Sox9 expression. Tissue Eng. 10: 145-155   DOI   ScienceOn
6 Stewart, M. C., K. M. Saunders, N. Burton-Wurster, and J. N. Macleod (2000) Phenotypic stability of articular chondrocytes in vitro: The effects of culture models, bone morphogenetic protein 2, and serum supplementation. J. Bone Miner. Res. 15: 166-174   DOI   ScienceOn
7 Lim, S. M., Y. S. Choi, H. C. Shin, C. W. Lee, and D.-I. Kim (2005) Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis. Biotechnol. Lett. 27: 607-611   DOI
8 Kim, E. J., W. J. Rhee, and T. H. Park (2001) Isolation and characterization of an apoptosis-inhibiting component from the hemolymph of Bombyx mori. Biochem. Biophys. Res. Commun. 285: 224-228   DOI   ScienceOn
9 Thirion, S. and F. Berenbaum (2004) Culture and phenotyping of chondrocytes in primary culture. Methods Mol. Med. 100: 1-14
10 Bassleer, C., L. Rovati, and P. Franchimont (1998) Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro. Osteoarthritis Cartilage 6: 427-434   DOI   ScienceOn
11 Oh, I. S. and H. G. Kim (2004) Vascular endothelial growth factor upregulates follistatin in human umbilical vein endothelial cells. Biotechnol. Bioprocess Eng. 9: 201-206   DOI   ScienceOn
12 Dodge, G. R. and S. A. Jimenez (2003) Glucosamine sulfate modulates the levels of aggrecan and matrix metalloproteinase- 3 synthesized by cultured human osteoarthritis articular chondrocytes. Osteoarthritis Cartilage 11: 424-432   DOI   ScienceOn
13 Barry, F., R. E. Boynton, B. Liu, and J. M. Murphy (2001) Chondrogenic differentiation of mesenchymal stem cells from bone marrow: Differentiation-dependent gene expression of matrix components. Exp. Cell Res. 268: 189-200   DOI   ScienceOn
14 Chen, H. C., H. P. Lee, M. L. Sung, C. J. Liao, and Y. C. Hu (2004) A novel rotating-shaft bioreactor for two-phase cultivation of tissue-engineered cartilage. Biotechnol. Prog. 20: 1802-1809   DOI   ScienceOn
15 Kim, J. E., E. J. Kim, W. J. Rhee, and T. H. Park (2005) Enhanced production of recombinant protein in Escherichia coli using silkworm hemolymph. Biotechnol. Bioprocess Eng. 10: 353-356   과학기술학회마을   DOI   ScienceOn
16 Freed, L. E., R. Langer, I. Martin, N. R. Pellis, and G. Vunjak-Novakovic (1997) Tissue engineering of cartilage in space. Proc. Natl. Acad. Sci. USA 94: 13885-13890
17 Philp, D., S. S. Chen, W. Fitzgerald, J. Orenstein, L. Margolis, and H. K. Kleinman (2005) Complex extracellular matrices promote tissue-specific stem cell differentiation. Stem Cells 23: 288-296   DOI   ScienceOn
18 Girotto, D., S. Urbani, P. Brun, D. Renier, R. Barbucci, and G. Abatangelo (2003) Tissue-specific gene expression in chondrocytes grown on three-dimensional hyaluronic acid scaffolds. Biomaterials 24: 3265-3275   DOI   ScienceOn
19 Short, B., N. Brouard, T. Occhiodoro-Scott, A. Ramakrishnan, and P. J. Simmons (2003) Mesenchymal stem cells. Arch. Med. Res. 34: 565-571   DOI   ScienceOn
20 Yaeger, P. C., T. L. Masi, J. L. de Ortiz, F. Binette, R. Tubo, and J. M. McPherson (1997) Synergistic action of transforming growth factor-beta and insulin-like growth factor- I induces expression of type II collagen and aggrecan genes in adult human articular chondrocytes. Exp. Cell Res. 237: 318-325   DOI   ScienceOn
21 Kipnes, J., A. L. Carlberg, G. A. Loredo, J. Lawler, R. S. Tuan, and D. J. Hall (2003) Effect of cartilage oligomeric matrix protein on mesenchymal chondrogenesis in vitro. Osteoarthritis Cartilage 11: 442-454   DOI   ScienceOn
22 Thomas, B., S. Thirion, L. Humbert, L. Tan, M. B. Goldring, G. Bereziat, and F. Berenbaum (2002) Differentiation regulates interleukin-1beta-induced cyclooxygenase- 2 in human articular chondrocytes: role of p38 mitogen-activated protein kinase. Biochem. J. 362: 367-373   DOI