• Microbiology and Biotechnology Letters
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• v.29 no.3
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• pp.142-148
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• 2001

An indigenous antagonistic bacterium Bacillus sp. 7079 was isolated from a local soil sampled at Kyongju area in Korea . The strain has strong antagonistic ability which was originated from multifunctional mechanisms of chitinase and antibiotic and is a powerful antagonistic biocontrol agent against red-pepper rotting fungus Phytophthora capsici and Wilt fungus Fusarium oxysporum. The chitinase might degrade the cell wasll for Fusarium species. The selected Bacilus sp. 7079 was identified as a Bacillus amyloliquefaciens 7079. The maximal production of the chitinase from B, amyloliquefaciens 7079 were obtained in chitin-yeast extract medium containing 0.7%, $K_2$$HPO_4$, $0.2KH_2$$PO_4$, 0.1% ($NH_4$)$_2$$SO_4$, 0.05% sodium cirate, 0.01% $MgSO_4$$7H_2$O, 0.1% yeast extract and 0.1% colloidal chitin after cultivation of 3 days at pH 7.0 and $30^{\circ}C$. The best carbon and nitrogen sources for the production of the chitinase from B amyloliquefaciens 7079 were determined to be 0.1% colloi- dal chitin and 0.15% proteose peptone NO 3 respectively, The antagonistic activity of B amyloliquefaciens 7079 was confirmed using P. capsici by in vivo pot test with red-pepper plant.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.337-342
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• 2009

Present studies were conducted to evaluate the effects of medium strength(MS and Hyponex), carbon sources and their concentrations, agar concentrations, and inoculation amounts on prothallus propagation of Pterdium aquilinum var. latiusculum(Desv.) Underw. ex Hell in vitro. The optimum MS medium strength for prothallus propagation was 2MS concentration. Phosphate source was most effective for prothallus growth of P. aquilinum var. latisculum. The addition of 1% sucrose or glucose to MS medium promoted prothallus multiplication. Growth of prothallus was not affected by agar concentration. Propagation of homogenized prothallus was vigorous even in liquid medium. Chopped gametophytes(100 and 200 mg) were inoculated on 250 ml ${\Delta}$flask with 100 mL of 2MS concentration medium and suspension culture was done at 100 rpm for 22 days. After 20 days, prothallus multiplication slowed down, so 100 mg of chopped prothalli is recommended for initial inoculation, since initial amount of inoculum did not affect subsequent prothallus multiplication. Consequently after 20 days of suspension culture, prothallus should be subcultured or transplanted outside of growing vessels.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.42-47
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• 1987

4 strains of the oak mushroom Lentinus edodes including the strain ASI 3046 were collected home and abroad to investigate the characterics of mycelial growth on synthetic media and sawdust media. The possibility of artificial culture of Lentinus edodes on media composed of different saw dusts and adding materials was investigated. Results were as follows: The optimum temperature for growth ranged from $22^{\circ}C$ to $26^{\circ}C$ among the lineages of Lentinus edodes. The optimal growth temperature for ASI 3046 strain and ASI 3047 strain was $26^{\circ}C$ and $22^{\circ}C$, respectively. The optimal carbon source for ASI 3046 strain was glucose, and peptone and ammoinum tartrate were proved excellent as nitrogen sources. When Lentinus edodes was cultured on sawdust media by varying the mixing ratio of different sawdusts and adding materials, mixed sawdust media composed of oak sawdust(50%, v/v), poplar sawdust(50%, v/v), acorn powder(5%, v/v) and rice bran(10%, v/v) showed higher yields of fruit bodies. Results showed that the ideal strain of Lentinus edodes for artificial culture on sawdust media was strain ASI 3046.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.16-21
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• 2008

This study was carried out to obtain the basic data for the mycelial growth conditions of cauliflower mushroom, Sparassis crispa. Twenty-one isolates were collected from domestic and abroad. The optimal temperature and pH for the mycelial growth of these isolates were $23^{\circ}C$ and pH 6.0, respectively. The mycelial growth was the best in the HBA medium, but very poor in the Lilly medium. However no mycelial growth in the CzapekDox medium. The utilization of carbon source was the best with fructose, and that of nitrogen source was the best with glutamine when compared to other tested sources. The selected isolate ASI150010 produced the highest mycelial weight in liquid culture containing soybean mill ($15\;g/{\ell}$) and potato ($200\;g/{\ell}$) extract. And uncleaned rice, wheat and barley were found to be good substrates for the mycelial growth S. crispa.

• Proceedings of the Korean Environmental Health Society Conference
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• 2005.06a
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• pp.254-258
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• 2005

The purpose of this study is to characterize the indoor-outdoor relationship of airborne pollutants and recognize probable sources in inside and outside individual apartments in Seoul metropolitan. Simultaneous air monitoring in inside and outside of the 16 Korean Apartments classified into 2groups: less than 1 year old and more than 4 years old from October, 2004 to February, 2005were sampled f3r airborne pollutants(volatile organic compounds, formaldehyde, respiratory particles, carbon dioxide and bacteria) using the Korean Indoor Air Quality Official Method. The concentrations of $CO_2$, TVOCs, HCHO, bacteria and PM10 in the less than 1 year old apartments were determined to be $773.6{\pm}422.3ppm$, $4,393.8{\pm}2,758.2{\mu}g/m^3$, $98.0{\pm}56.4{\mu}g/m^3$, $254.0{\pm}186.3CFU/m^3$ and $31.7{\pm}14.8{\mu}g/m^3$, respectively, Also, the concentrations of those in the more than 4 years old apartments were determined to be $798.9{\pm}266.5ppm$, $792.7{\pm}398.3{\mu}g/m^3$, $70.0{\pm}30.7{\mu}g/m^3$, $245.6{\pm}122.0CFU/m^3$, $49.7f28.7{\pm}g/m^3$, respectively. The average ratios of the indoor and outdoor concentrations of $CO_2$, TVOCs, HCHO, bacteria and PM10 were 2.2, 3.6, 3.1, 3.9 and 1.4, respectively. These results of this analysis is suggested that $CO_2$, TVOCs, HCHO, bacteria and PM10 in indoor air are both emitted from source within the apartment environment and partly come from outdoor air. With the above considerations in mind, it is suggested that the research for source contribution of indoor air pollutants should be expanded and the detailed interpretation of the results on these needed further study(using principal component analysis(PCA).

• Journal of environmental and Sanitary engineering
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• v.18 no.3 s.49
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• pp.100-109
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• 2003

Recently, Indoor air quality(IAQ) in workplace, residential environments and schools has been concern of people, scientists and related the public. And so in Seoul has recognized the healthy effect related to IAQ in schools. Therefore, the objective of this study reported in this article were to measure and compare the perception of IAQ of selected air pollutants at three different schools in Seoul. We performed a questionnaire survey of 400 students about their awareness for the importance of IAQ in our school. And we measured the IAQ of 3 schools considering as site region, construction year and studying level. The indoor air pollutants and parameters such as temperature, relative humidity, respirable particulate matter(PM10), formaldehyde(HCHO), total bacteria counts(TBC), carbon dioxide(CO$_2$), and noise were monitored in indoors. In results, all most response of occupant has recognized the awareness of IAQ at schools. The PMIO, TBC and Noise level of all schools were higher than the standard of the public 150 ${mu}$g/m$^3$ and 500CFU/m$^3$, the level formaldehyde(HCHO) was below 0.1 ppm of the healthy guideline of Korea And the concentration of CO$_2$ were investigated below 1,000 ppm of the standard implying ventilation in 2 schools except for 1 school(c school). Finally, the control of most important pollutants of IAQ in school were PM10, TBC and Noise. Therefore, it can be concluded that the indoor air quality of selected 3 schools studied was perceived as acceptable, it is recommended that the government related IAQ was suggested the guideline and control of IAQ problems in schools, and all member relating school need to be effort to reduce the exposure of sources to undesirable indoor pollutants such as Particlate and Noise.

• Journal of Mushroom
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• v.14 no.4
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• pp.184-190
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• 2016

This study was performed to determine the optimal condition for Lentinula edodes JMI-10079 mycelium cultivation on the root of Polygonum multiflorum. We also analyzed the proximate composition, total amino acids, and minerals in the root of P. multiflorum cultivated with L. edodes JMI-10079 mycelia. The optimal temperature and pH for L. edodes JMI-10079 mycelium cultivation on the P. multiflorum root were $25^{\circ}C$ and pH 5-6 respectively, whereas the optimal carbon and nitrogen sources were glucose and maltose, respectively. The content of crude protein, crude fat, and ash in the P. multiflorum root cultivated with L. edodes JMI-10079 mycelia was higher than that in the uncultivated P. multiflorum root. The content of crude fiber was the highest in the control. Total amino acid analysis revealed that the contents of total amino acids and total essential amino acids were increased by higher root of P. multiflorum concentration.

• Journal of Mushroom
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• v.15 no.4
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• pp.264-268
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• 2017

In the 21st century, information and communication technology (ICT) worldwide presents a new vision for agriculture. Time and place, as well as the high-tech industry, to overcome barriers to the fusion of the so-called "smart agriculture," are changing the agricultural landscape. Core container production in precision agriculture for mushroom cultivation, optimal temperature, humidity, irradiation, self-regulation of factors such as carbon dioxide, and environment for mushroom cultivation were adopted. Lentinula edodes (shiitake) is an edible mushroom native to East Asia, cultivated and consumed in many Asian countries. It is considered to be medicinal in certain practices of traditional medicine. We used different controlled light sources (Blue-Red-White-combined LED, blue LED, red LED, and fluorescent light) with different LED radiation intensities (1.5, 10.5, and $20.5{\mu}mol/m^2s$ for LEDs) to compare growth and development. Mushrooms were treated with light in a 12-hour-on/12-hour-off cycle, and maintained in a controlled room at $19{\sim}21^{\circ}C$, with 80~90% humidity, and an atmospheric $CO_2$ concentration of 1,000 ppm for 30 days. Growth and development differed with the LED source color and LED radiation intensity. Growth and development were the highest at $10.5{\mu}mol/m^2s$ of blue LED light. After harvesting the fruit bodies, we measured their weight and length, thickness of pileus and stipe, chromaticity, and hardness. The $10.5{\mu}mol/m^2s$ blue-LED-irradiated group showed the best harvest results with an average individual weight of 39.82 g and length of 64.03 mm, pileus thickness of 30.85 mm and pileus length of 43.22 mm, and stipe thickness of 16.96 mm with fine chromaticity and hardness. These results showed that blue LED light at $10.5{\mu}mol/m^2s$ s exerted the best effect on the growth and development of L. edodes (shiitake) mushroom in the ICT-system container-type environment.

• Journal of Soil and Groundwater Environment
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• v.8 no.4
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• pp.45-52
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• 2003

A gas-substrate degrading bacterium, Nocardia SW3, was isolated from the gasoline contaminated aquifer using propane and butane as carbon and energy sources. We have examined the effects of substrate concentration, temperature and pH on the gas substrate degradation as well as MTBE cometabolic degradation. The result for the effect of substrate concentration showed that the maximum degradation rates of propane and butane were 30.6 and 25.4 (n㏖/min/mg protein) at 70 $\mu$㏖, respectively. The optimum temperature and pH for the degradation of gas substrate were $30^{\circ}C$ and 7, respectively. Substrate degradation activity, however, was still active in broad range of pH from 5 to 8 and temperature between $15^{\circ}C$and$35^{\circ}C$. The degradation activity of Nocardia SW3 for the MTBE was similar to the both substrates. The observed maximal transformation yields ($T_y$) were 46.7 and 35.0 (n㏖ MTBE degraded $\mu$㏖ substrate utilized), and the maximal transformation capacities ($T_c$) were 320 and 280 (n㏖MTBE degraded/mg biomass used) for propane and butane oxidizing activity on MTBE, respectively. And also, TBA was detected as by-product of MTBE and it was continuously degraded further.

• Journal of the Korean Society of Groundwater Environment
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• v.7 no.1
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• pp.55-58
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• 2000

Methyl tertiary butyl ether (MTBE) is a gasoline additive that boosts the oxygen content in fuel (an oxygenate), resulting in less air-polluting carbon monoxide being released from vehicle exhaust systems. Then, groundwater contamination problems have been developed in areas where the chemical is used. Common sources of water contamination by MTBE include leaking underground gasoline storage tanks and leaks and spills from above ground fuel storage tanks, etc.. Studies on the chemical and these problems are going on abroad vigorously. These studies should be performed in our country as well more actively. This paper reviews on articles on these studies and focuses on the identification of the chemical as a groundwater contamination source.