Studies on the Artificial Cultivation of Lentinus edodes on sawdust media

표고버섯 톱밥 인공재배(人工栽培)에 관(關)한 연구(硏究)

4 strains of the oak mushroom Lentinus edodes including the strain ASI 3046 were collected home and abroad to investigate the characterics of mycelial growth on synthetic media and sawdust media. The possibility of artificial culture of Lentinus edodes on media composed of different saw dusts and adding materials was investigated. Results were as follows: The optimum temperature for growth ranged from $22^{\circ}C$ to $26^{\circ}C$ among the lineages of Lentinus edodes. The optimal growth temperature for ASI 3046 strain and ASI 3047 strain was $26^{\circ}C$ and $22^{\circ}C$, respectively. The optimal carbon source for ASI 3046 strain was glucose, and peptone and ammoinum tartrate were proved excellent as nitrogen sources. When Lentinus edodes was cultured on sawdust media by varying the mixing ratio of different sawdusts and adding materials, mixed sawdust media composed of oak sawdust(50%, v/v), poplar sawdust(50%, v/v), acorn powder(5%, v/v) and rice bran(10%, v/v) showed higher yields of fruit bodies. Results showed that the ideal strain of Lentinus edodes for artificial culture on sawdust media was strain ASI 3046.

표고버섯의 배양적 특성과 톱밥수종 및 첨가재료를 이용(利用)한 인공재배(人工栽培) 가능성(可能性)을 검토한 결과(結果)는 다음과 같다. 1. 표고버섯의 톱밥인공재배(人工栽培)에 적합한 균주(菌株)는 ASI3046 균주(菌株)이며 균계생육(菌系生育) 최저온도(最低溫度)는 $22{\sim}26^{\circ}C$, pH는 $5.5{\sim}6.5$ 이였다. 2. ASI3046 균주(菌株)에 대한 균계생육(菌系生育)이 양호(良好)한 탄소원(炭素源)은 glucose이며 질소원(窒素源)은 peptone과 ammonium tartrate 였다. 3. 표고버섯 통밥인공재배시(人工栽培時) 배지(培地)의 수종 및 첨가재료(添加材料)는 혼합(混合)통밥(참나무톱밥+포플라톱밥)에 상수리 분말 5% (v/v)에 미강 10% (v/v)를 첨가(添加)한 구에서 군계생장(菌系生長) 및 균계밀도(菌系密度)가 양호(良好)하고 자실본수량도 높았다. 4. 표고버섯 통밥인공재배(人工栽培)에 적합한 균주(菌株)는 ASI3046 균주(菌株) 이였다.