Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Chemical compositions of fermented Polygonum multiflorum Thunberg. root by Lentinula edodes (Berk.) Pegler mycelials cultivation

표고균사체 발효하수오 배양적 특성 및 이화학적 성분

  • Oh, Junseok (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Hong, Jae-Heoi (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Park, Tae-Young (Dongbu Eastern Herbal Medicine Agricultural Association Corporation) ;
  • Kim, Kyung-Je (Jangheung Research Institute for Mushroom Industry) ;
  • Jin, Seong-Woo (Jangheung Research Institute for Mushroom Industry) ;
  • Ban, Seung-Eon (Jangheung Research Institute for Mushroom Industry) ;
  • Koh, Young-Woo (Jangheung Research Institute for Mushroom Industry) ;
  • Jeong, Sang-Wook (Jangheung Research Institute for Mushroom Industry) ;
  • Im, Seung-Bin (Jangheung Research Institute for Mushroom Industry) ;
  • Seo, Kyoung-Sun (Jangheung Research Institute for Mushroom Industry)
  • 오준석 (동부생약영농조합법인) ;
  • 홍재희 (동부생약영농조합법인) ;
  • 박태영 (동부생약영농조합법인) ;
  • 김경제 ((재)장흥군버섯산업연구원) ;
  • 진성우 ((재)장흥군버섯산업연구원) ;
  • 반승언 ((재)장흥군버섯산업연구원) ;
  • 고영우 ((재)장흥군버섯산업연구원) ;
  • 정상욱 ((재)장흥군버섯산업연구원) ;
  • 임승빈 ((재)장흥군버섯산업연구원) ;
  • 서경순 ((재)장흥군버섯산업연구원)
  • Received : 2016.11.29
  • Accepted : 2016.12.19
  • Published : 2016.12.31
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Abstract

This study was performed to determine the optimal condition for Lentinula edodes JMI-10079 mycelium cultivation on the root of Polygonum multiflorum. We also analyzed the proximate composition, total amino acids, and minerals in the root of P. multiflorum cultivated with L. edodes JMI-10079 mycelia. The optimal temperature and pH for L. edodes JMI-10079 mycelium cultivation on the P. multiflorum root were $25^{\circ}C$ and pH 5-6 respectively, whereas the optimal carbon and nitrogen sources were glucose and maltose, respectively. The content of crude protein, crude fat, and ash in the P. multiflorum root cultivated with L. edodes JMI-10079 mycelia was higher than that in the uncultivated P. multiflorum root. The content of crude fiber was the highest in the control. Total amino acid analysis revealed that the contents of total amino acids and total essential amino acids were increased by higher root of P. multiflorum concentration.

본 연구는 하수오 표고균사(Lentinula edodes JMI-10079) 배양 최적조건 탐색을 목적으로 수행하였으며, 표고균사로 배양된 하수오의 일반성분, 아미노산, 무기성분을 분석하였다. 하수오 표고균사 배양 최적온도는 생장속도는 $25^{\circ}C$ 였으며, pH 5~8의 범위에서 균사생장이 양호하였다. 하수오 표고균사 배양 최적 탄소원은 glucose였으며, 질소원은 malt extract로 나타났다. 조단백질 함량은 적하수오 첨가량이 증가함에 따라 증가하는 경향을 보였다. 조지방 함량과 조회분 함량은 적하수오 발효물 첨가량에 비례하여 증가하는 경향을 보였다. 조섬유 함량은 대조구에서 36.39%로 가장 높게 나타났다. 16종의 아미노산이 검출되었으며, 총 구성아미노산 및 필수아미노산의 함량은 적하수오 발효물 첨가량에 비례하여 증가하는 경향을 확인 하였다. 본 연구는 하수오 100%에서도 표고균사배양이 원활하게 이루어져 향 후 대량생산을 시도할 때 공정조건이 간편할 것으로 보인다. 성분 분석 결과에서도 하수오 첨가량이 증가함에 따라, 조단백질, 조회분, 총 아미노산 및 potassium을 제외한 무기성분 함량이 증가함을 확인하여, 향 후 표고균사 배양 하수오를 이용한 식품개발이 기대되어 진다.

Keywords

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