• Journal of Microbiology and Biotechnology
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• 제18권11호
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• pp.1755-1761
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• 2008

The present study revealed that Lrp, a leucine-responsive regulatory protein, is involved in the regulation of cadBA transcription through activation of $P_{cadBA}$. The influence of Lrp on $P_{cadBA}$ was mediated by CadC, and thereby, CadC was able to compensate for the lack of Lrp in the activation of $P_{cadBA}$. Western blot analyses and EMSA demonstrated that the cellular level of CadC was not significantly affected by Lrp, and that Lrp exerted its effect by directly binding to $P_{cadBA}$. These combined results suggested that CadC and Lrp function cooperatively to activate the $P_{cadBA}$ rather than sequentially in a regulatory cascade.

• Bulletin of the Korean Chemical Society
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• 제26권8호
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• pp.1185-1189
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• 2005

Two new copper vanadium borophosphate compounds, $(NH_4)(C_2H_{10}N_2)_{5.5}[Cu(C_2H_8N_2)_2]_3[V_2P_2BO_{12}]_6{\cdot}17H_2O,\;Cu-VBPO1\;and\;(NH_4)(C_2H_{10}N_2)_{3.5}[Cu(C_2H_8N_2)_2]_5[V_2P_2BO_{12}]_6{\cdot}18H_2O$, Cu-VBPO2 have been hydrothermally synthesized and characterized by single crystal X-ray diffraction, thermogravimetric analysis, IR spectroscopy, and elemental analysis. The structure of Cu-VBPO1 contains a layer anion, {$[Cu(C_2H_8N_2)_2]_3[V_2P_2BO_{12}]_6$}$^{12-}$, whereas Cu-VBPO2 has an open framework anion, {$[Cu(C_2H_8N_2)_2]_5[V_2P_2BO_{12}]_6$}$^{8-}$. Crystal Data: $(NH_4)(C_2H_{10}N_2)_{5.5}[Cu(C_2H_8N_2)_2]_3[V_2P_2BO_{12}]_6{\cdot}17H_2O$, monoclinic, space group I2/m (no. 12), $\alpha$ = 15.809(1) $\AA$, b = 31.107(2) $\AA$, c = 12.9343(8) $\AA$, $\beta$ = 104.325(1)$^{\circ}$, Z = 2; $(NH_4)(C_2H_{10}N_2)_{3.5}[Cu(C_2H_8N_2)_2]_5[V_2P_2BO_{12}]_6{\cdot}18H_2O$, tetragonal, space group $P4_2$/mnm (no.136), $\alpha$ = 26.832(1) $\AA$, c = 18.021(1) $\AA$, Z = 4.

• 한국미생물·생명공학회지
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• 제37권2호
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• pp.99-104
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• 2009

Streptomyces coelicolor A3(2)의 $\beta$-glucosidase 유전자를 분리하여 대장균에서 발현하여 특성을 조사하였다. 최적 활성을 나타내는 온도는 pH 5에서는 $20^{\circ}C$, pH 6에서는 $60^{\circ}C$에서 높은 활성을 나타냈다. pH에 따른 활성은 pH 3 이하와 pH 9 이상의 범위에서는 낮은 활성을 나타냈으며 pH 7에서 가장 높은 활성을 나타냈다. $\alpha$-pNPG($\rho$-nitrophenyl-$\alpha$-D-glucopyranoside), $\beta$-pNPG ($\rho$-nitrophenyl-$\beta$-D-glucopyranoside), $\beta$-pNPF($\\rho$-nitrophenyl-$\beta$-D-fucopyranoside)는 pH 3-10까지 비슷한 활성을 나타냈으며, $\alpha$-pNPG가 pH 7에서 다소 높은 활성을 보였다. $\beta$-pNPGA는 pH 5-9까지 높은 활성을 나타냈으며, 특히 pH 9에서 3배 이상의 높은 활성을 나타냈다. 기질 $\alpha$-pNPG, $\beta$-pNPG, $\beta$-pNPF의 온도에 따른 활성변화는 $\beta$-pNPF의 활성이 $60^{\circ}C$에서 증가하였고, $\beta$-pNPGA는 $30-50^{\circ}C$까지 활성이 증가하여 $50^{\circ}C$에서 최대활성을 나타내었다. 당화 flavonoid를 이용한 기질특이성의 상대활성은 daidzin, glycitin, genistin, 순으로 나타났으며 esculin과 apigenin-7-glucose는 기질로 사용하지 않았다. $\beta$-Glucosidase 활성은 EDTA, DTT에 의해 억제되었으며, $MnSO_4$, $CaCl_2$, KCl, $MgSO_4$에 의해 증가하였고, 특히 Mn이온에 의해 증가하였다. $CuSO_4$, NaCl에 의해 효소활성이 저해되었으며, 특히 $ZnSO_4$의 경우 효소활성이 강하게 억제되었다.

• 대한치과기공학회지
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• 제9권1호
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• pp.21-29
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• 1987

The purpose of this investigation was to determine the effect of recasting on the castability and physical properties of the nonprecious metal alloy. Using 2 sorts of the nonprecious metal alloy for production of Crown and Bridge prosthetics, such as 1) sankin C. B 80 metal 2) C & B alloy, the result of experimental research on the castability and hardness according to the recasting, is as followes; 1. The difference of castability between sankin CB80 metal and C & B alloy was statistically significant (p < 0.01) and the difference of castability among the test groups, was statistically significant (p <0.01). 2. In the case of sankin C.B 80 metal, the difference of castability among the New alloy and first mixed alloy and second mixed alloy and old alloy was not significant statistically (p >0.05). 3. In the case of C & B alloy, the difference of castability among the New alloy and mixed alloys and old alloy, was not significant statistically (p >0.05). 4. The difference of hardness between sankin C & B alloy was not significant statistically (p >0.05), but the difference of hardness among the test groups was statistically significant (p <0.01). 5. In the case of Sankin C.B 80 metal, the difference of hardness among the new ally and mixed alloys and old alloy was not significant statistically (p >0.05). 6. In the case of C & B alloy, the difference of hardness between New alloy and mixed alloys was not significant statistically (p >0.05) but the difference of hardness among the old alloy and New alloy and mixed alloys was statistically significant (p <0.01).

• 한국동물학회지
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• 제39권3호
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• pp.248-256
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• 1996

본 연구의 목적은 인간의 Poly(ADP-ribose) synthetase (PARS)의 cDNA를 클로닝하여 발현시키기 위해 수행하였다. 먼저, 인간의 PARS cDNA 전체를 포함한 pPARS3.1을 pGEM-7Zf(+) 등의 발현 벡터 클로닝하였다. 이 결과로 생성된 재조합 플라스미드 pPARS6.1이 인간의 PARS cDNA 전체를 포함하고, 올바른 방향으로 삽입되었는지를 확인하기 위해 제한효소 지도를 작성하였고, random primed DNA probe을 이용한 Southern blot 분석에 의해서 PARS가 클로닝되었다는 것을 확인하였다. 또한, 염기서열 분석 결과, 단백질 합성이 시작되는 유전 암호가 정확한 순서로 위치하고 있음을 확인하였다. 재조합된 pPARS6.1의 발현을 위해 배양시 0.4 mM IPTG로 처리하여 만들어진 인간의 PARS 단백질이 10% SDS-PAGE에서 120 kDa 위치에 이동하였다는 것을 nick-translated된 DNA를 probe로 이용하여 확인하였고, Southwestern blot 실험 결과 120 kDa과 98kDa에 위치하는 단백질이 DNA와 결합함을 알 수 있었다.

• Wind and Structures
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• 제26권5호
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• pp.331-341
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• 2018

A numerical study on the flow over a square cylinder in the vicinity of a wall is conducted for different Couette-Poiseuille-based non-uniform flow with the non-dimensional pressure gradient P varying from 0 to 5. The non-dimensional gap ratio L (=$H^{\ast}/a^{\ast}$) is changed from 0.1 to 2, where $H^{\ast}$ is gap height between the cylinder and wall, and $a^{\ast}$ is the cylinder width. The governing equations are solved numerically through finite volume method based on SIMPLE algorithm on a staggered grid system. Both P and L have a substantial influence on the flow structure, time-mean drag coefficient ${\bar{C}}_D$, fluctuating (rms) lift coefficient ($C_L{^{\prime}}$), and Strouhal number St. The changes in P and L leads to four distinct flow regimes (I, II, III and IV). Following the flow structure change, the ${\bar{C}}_D$, $C_L{^{\prime}}$, and St all vary greatly with the change in L and/or P. The ${\bar{C}}_D$ and $C_L{^{\prime}}$ both grow with increasing P and/or L. The St increases with P for a given L, being less sensitive to L for a smaller P (< 2) and more sensitive to L for a larger P (> 2). A strong relationship is observed between the flow regimes and the values of ${\bar{C}}_D$, $C_L{^{\prime}}$ and St. An increase in P affects the pressure distribution more on the top surface than on bottom surface while an increase in L does the opposite.

• JSTS:Journal of Semiconductor Technology and Science
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• 제5권2호
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• pp.89-101
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• 2005

Research on highly abstracted system modeling and simulation has received a great deal of attention as of the concept of platform based design is becoming ubiquitous. From a practical design point of view, such modeling and simulation must consider the following: (i) fast simulation speed and cycle accuracy, (ii) early availability for early stage software development, (iii) inter-operability with external tools for software development, and (iv) reusability of the models. Unfortunately, however, all of the previous works only partially addresses the requirements, due to the inherent conflicts among the requirements. The objective of this study is to develop a new system design methodology to effectively address the requirements mentioned above. We propose a new transaction-level system modeling methodology, called ViP (Virtual Platform). We propose a two-step approach in the ViP method. In phase 1, we create a ViP for early stage software development (before RTL freeze). The ViP created in this step provides high speed simulation, lower cycle accuracy with only minor modeling effort.(satisfying (ii)). In phase 2, we refine the ViP to increase the cycle accuracy for system performance analysis and software optimization (satisfying (i)). We also propose a systematic ViP modeling flow and unified interface scheme based on utilities developed for maximizing reusability and productivity (satisfying (ii) and (iv)) and finally, we demonstrate VChannel, a generic scheme to provide a connection between the ViP and the host-resident application software (satisfying (iii)). ViP had been applied to several System-on-a-chip (SoC) designs including mobile applications, enabling engineers to improve performance while reducing the software development time by 30% compared to traditional methods.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2002년도 제9차 국제심포지움 및 추계정기학술발표회
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• pp.58-58
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• 2002

아위버섯(Pleurotus Ferulae Lanzi)의 온도별 균사생장도를 조사한 결과, 균사생육온도는 4$^{\circ}C$~35$^{\circ}C$로 나타났으며, 최적 균사생장 온도는 $25^{\circ}C$였고, $25^{\circ}C$~32$^{\circ}C$에서도 비교적 균사생장이 양호하였다. pH별 균사생장도를 조사한 결과는 pH가 4.0, 5.0, 6.0, 7.0, 8.0으로 조절된 MYPA(Yeast Malt Peptone Arar)배지를 기본배지로 하여 균사생장을 조사한 결과, 최적 pH는 6.0이었고, 5.0, 7.0순으로 균사생장이 양호하였던 반면, 4.0에서는 균사생장이 극히 불량하였다. 아위버섯의 균사생장을 위한 최적 배지조성은 탄소원별 균사생장도를 조사한 결과, starch, maltose 첨가구에서 균사생장이 가장 양호하였다. 또한, fructose, glucose, mannitol, lactose, xylose 순으로 균사생장이 비교적 양호하였던 반면, 다른 탄소원에서는 불량하였다. 질소원별 균사 생장량을 조사한 결과 yeast 첨가구에서 균사생장이 가장 양호하였고, 반면, 다른 질소원에서는 불량하였다. 위의 내용을 종합해보면 아위버섯의 최적온도는 25~32$^{\circ}C$, 최적 pH는 5.0~6.0으로 나타났다 또한 적정 영양원은 기본배지로 선발된 MYPA에 탄소원은 starch, maltose, 질소원은 yeast extract을 첨가하는 것이 가장 좋게 나타났다.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.395-404
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• 2017

Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract from Paeonia lactiflora against Candida albicans, showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabling membrane depolarization, and compromising permeability. First, it was shown that the ethanol extract from P. lactiflora was involved in damaging the integrity of cell walls in C. albicans. In isotonic media, cell bursts of C. albicans by the P. lactiflora ethanol extract could be restored, and the minimum inhibitory concentration (MIC) of the P. lactiflora ethanol extract against C. albicans cells increased 4-fold. In addition, synthesis of $(1,3)-{\beta}-{\small{D}}-glucan$ polymer was inhibited by 87% and 83% following treatment of C. albicans microsomes with the P. lactiflora ethanol extract at their $1{\times}MIC$ and $2{\times}MIC$, respectively. Second, the ethanol extract from P. lactiflora influenced the function of C. albicans cell membranes. C. albicans cells treated with the P. lactiflora ethanol extract formed red aggregates by staining with a membrane-impermeable dye, propidium iodide. Membrane depolarization manifested as increased fluorescence intensity by staining P. lactiflora-treated C. albicans cells with a membrane-potential marker, $DiBAC_4(3)$ ((bis-1,3-dibutylbarbituric acid) trimethine oxonol). Membrane permeability was assessed by crystal violet assay, and C. albicans cells treated with the P. lactiflora ethanol extract exhibited significant uptake of crystal violet in a concentration-dependent manner. The findings suggest that P. lactiflora ethanol extract is a viable and effective candidate for the development of new antifungal agents to treat Candida-associated diseases.

• 광학세계
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• 통권140호
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• pp.34-35
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• 2012

대한민국 사진영상장비 대표기업 세기 P&C(주)(이봉훈 대표, www.saeki.co.kr)는 지난 6월 1일 서울 충무로 신사옥 오픈식을 개최했다. 세기P&C(주)는 지난 4월, 기존 종로구 와룡동에서 중구 충무로3가 소재의 신사옥으로 이전한 후, 동양 최대 규모의 멀티 브랜드숍을 표방한 '세기 브랜드숍'을 준비, 매장 구성을 완성하여 신사옥을 오픈했다. 이날 충무로 신사옥 오픈식에선 세기P&C(주) 이봉훈 대표의 자서전 출판기념회도 함께 진행되었다.