Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Activation of the Vibrio vulnificus cadBA Operon by Leucine-Responsive Regulatory Protein is Mediated by CadC

  • Rhee, Jee-Eun (Division of AIDS, Center for Immunology and Pathology, National Institute of Health) ;
  • Kim, Kun-Soo (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • Choi, Sang Ho (National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University)
  • Published : 2008.11.30
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Abstract

The present study revealed that Lrp, a leucine-responsive regulatory protein, is involved in the regulation of cadBA transcription through activation of $P_{cadBA}$. The influence of Lrp on $P_{cadBA}$ was mediated by CadC, and thereby, CadC was able to compensate for the lack of Lrp in the activation of $P_{cadBA}$. Western blot analyses and EMSA demonstrated that the cellular level of CadC was not significantly affected by Lrp, and that Lrp exerted its effect by directly binding to $P_{cadBA}$. These combined results suggested that CadC and Lrp function cooperatively to activate the $P_{cadBA}$ rather than sequentially in a regulatory cascade.

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References

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