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http://dx.doi.org/10.4014/jmb.0800.121

Activation of the Vibrio vulnificus cadBA Operon by Leucine-Responsive Regulatory Protein is Mediated by CadC  

Rhee, Jee-Eun (Division of AIDS, Center for Immunology and Pathology, National Institute of Health)
Kim, Kun-Soo (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Choi, Sang Ho (National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.11, 2008 , pp. 1755-1761 More about this Journal
Abstract
The present study revealed that Lrp, a leucine-responsive regulatory protein, is involved in the regulation of cadBA transcription through activation of $P_{cadBA}$. The influence of Lrp on $P_{cadBA}$ was mediated by CadC, and thereby, CadC was able to compensate for the lack of Lrp in the activation of $P_{cadBA}$. Western blot analyses and EMSA demonstrated that the cellular level of CadC was not significantly affected by Lrp, and that Lrp exerted its effect by directly binding to $P_{cadBA}$. These combined results suggested that CadC and Lrp function cooperatively to activate the $P_{cadBA}$ rather than sequentially in a regulatory cascade.
Keywords
Vibrio vulnificus; cadBA; CadC; Lrp;
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Times Cited By KSCI : 7  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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1 Busby, S. and R. H. Ebright. 1999. Transcription activation by catabolite activator protein (CAP). J. Mol. Biol. 293: 199-213   DOI   ScienceOn
2 Donnenberg, M. S. and J. B. Kaper. 1991. Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59: 4310-4317
3 Gulig, P. A., K. L. Bourdage, and A. M. Starks. 2005. Molecular pathogenesis of Vibrio vulnificus. J. Microbiol. 43: 118-131   과학기술학회마을
4 Linkous, D. A. and J. D. Oliver. 1999. Pathogenesis of Vibrio vulnificus. FEMS Microbiol. Lett. 174: 207-214   DOI   ScienceOn
5 Miller, J. F., J. J. Mekalanos, and S. Falkow. 1989. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243: 916-922   DOI
6 Salmon, K., S. P. Hung, K. Mekjian, P. Baldi, G. W. Hatfield, and R. P. Gunsalus. 2003. Global gene expression profiling in Escherichia coli K12. The effects of oxygen availability and FNR. J. Biol. Chem. 278: 29837-29855   DOI   ScienceOn
7 Newman, E. B., R. D'Ari, and R. T. Lin. 1992. The leucine-Lrp regulon in E. coli: A global response in search of a raison d'Etre. Cell 68: 617-619   DOI   ScienceOn
8 Rhee, J. E., H. G. Jeong, J. H. Lee, and S. H. Choi. 2006. AphB influences acid tolerance of Vibrio vulnificus by activating expression of the positive regulator CadC. J. Bacteriol. 188: 6490-6497   DOI   ScienceOn
9 Lee, J. H. and S. H. Choi. 2006. Coactivation of Vibrio vulnificus putAP operon by cAMP receptor protein and PutR through cooperative binding to overlapping sites. Mol. Microbiol. 60: 513-524   DOI   ScienceOn
10 Mekalanos, J. J. 1992. Environmental signals controlling expression of virulence determinants in bacteria. J. Bacteriol. 174: 1-7   DOI
11 Jeong, H. S. 2008. Unpublished data
12 Rhee, J. E., J. H. Rhee, P. Y. Ryu, and S. H. Choi. 2002. Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress. FEMS Microbiol. 208: 245-251   DOI   ScienceOn
13 Rhee, J. E., H. M. Ju, U. Park, B. C. Park, and S. H. Choi. 2004. Identification of the Vibrio vulnificus cadC and evaluation of its role in acid tolerance. J. Microbiol. Biotechnol. 14: 1093-1098
14 Strom, M. S. and R. N. Paranjpye. 2000. Epidemiology and pathogenesis of Vibrio vulnificus. Microbes Infect. 2: 177-188   DOI   ScienceOn
15 Phan, A. P. H., T. T. Ngo, and H. M. Lenhoff. 1982. Spectrophotometric assay for lysine decarboxylase. Anal. Biochem. 120: 193-197   DOI   ScienceOn
16 Choi, H. K., N. Y. Park, D. Kim, H. J. Chung, S. R. Ryu, and S. H. Choi. 2002. Promoter analysis and regulatory characteristics of vvhBA encoding cytolytic hemolysin of Vibrio vulnificus. J. Biol. Chem. 277: 47292-47299   DOI   ScienceOn
17 Park, N. Y., J. H. Lee, B. C. Lee, T. S. Kim, and S. H. Choi. 2006. Identification and characterization of the wbpO gene essential for lipopolysaccharide synthesis in Vibrio vulnificus. J. Microbiol. Biotechnol. 16: 808-816   과학기술학회마을
18 Park, N. Y., J. H. Lee, M. W. Kim, H. G. Jeong, B. C. Lee, T. S. Kim, and S. H. Choi. 2006. Identification of Vibrio vulnificus wbpP gene and evaluation of its role in virulence. Infect. Immun. 74: 721-728   DOI   ScienceOn
19 Jeong, H. S., J. E. Rhee, J. H. Lee, H. K. Choi, D. I. Kim, M. H. Lee, S. J. Park, and S. H. Choi. 2003. Identification of Vibrio vulnificus lrp and its influence on survival under various stresses. J. Microbiol. Biotechnol. 13: 159-163
20 Cui, Y., Q. Wang, G. D. Stormo, and J. M. Calvo. 1995. A consensus sequence for binding of Lrp to DNA. J. Bacteriol. 177: 4872-4880   DOI
21 Oka, A., H. Sugisaki, and M. Takanami. 1981. Nucleotide sequence of the kanamycin resistance transposon Tn903. J. Mol. Biol. 147: 217-226   DOI
22 Lee, J. H., J. E. Rhee, U. R. Park, H. M. Ju, B. C. Lee, T. S. Kim, H. S. Jeong, and S. H. Choi. 2007. Identification and functional analysis of Vibrio vulnificus smcR a novel global regulator. J. Microbiol. Biotechnol. 17: 325-334   과학기술학회마을
23 Lee, J. H., S. Y. Jeong, and S. H. Choi. 2006. Regulatory characteristics of the Vibrio vulnificus putAP operon encoding proline dehydrogenase and proline permease. J. Microbiol. Biotechnol. 16: 1285-1291   과학기술학회마을
24 Rhee, J. E., K. S. Kim, and S. H. Choi. 2005. CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. J. Bacteriol. 187: 7870-7875   DOI   ScienceOn
25 Lee, J. H., M. W. Kim, B. S. Kim, S. M. Kim, B. C. Lee, T. S. Kim, and S. H. Choi. 2007. Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice. J. Microbiol. 45: 146-152   과학기술학회마을
26 Weyand, N. J., B. A. Braaten, M. Van der Wooude, J. Tucker, and D. A. Low. 2001. The essential role of the promoterproximal subunit of CAP in pap phase variation: Lrp- and helical phase-dependent activation of papBA transcription by CAP from -215. Mol. Microbiol. 39: 1504-1522   DOI   ScienceOn
27 Jeong, H. S., M. H. Lee, K. H. Lee, S. J. Park, and S. H. Choi. 2003. SmcR and cyclic AMP receptor protein coactivate Vibrio vulnificus vvpE encoding elastase through the RpoS-dependent promoter in a synergistic manner. J. Biol. Chem. 278: 45072-45081   DOI   ScienceOn
28 Milton, D. L., R. O'Toole, P. Horstedt, and H. Wolf-Watz. 1996. Flagellin A is essential for the virulence of Vibrio anguillarium. J. Bacteriol. 178: 1310-1319   DOI