• Title/Summary/Keyword: c-kit

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First record of Diplazium mettenianum (Miq.) C. Chr. var. metteniamum (Athyriaceae) from Korea (한반도 미기록 식물: 깃주름고사리(개고사리과))

  • Lee, Chang Shook;Lee, Kanghyup;Ko, Sun Yeul
    • Korean Journal of Plant Taxonomy
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    • v.44 no.4
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    • pp.242-246
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    • 2014
  • The newly recorded species, Diplazium mettenianum (Miq.) C. Chr. var. mettenianum (Athyriaceae) was collected from a forest in Min-oreum, Jeju-do. D. mettenianum var. mettenianum (vernacular name: 'Kit-ju-reum-go-sa-ri') is distinguished from other Korean congeners of the genus Diplazium by having pinnae shallowly or halfway lobed, lower pinnae stalked, lanceolate, pinna segments serrate margin and obtuse apex. A new Korean name, 'Kit-ju-reum-go-sa-ri', was given based on the shape of pinna halfway lobed. Descriptions and illustrations of this taxon and its photograph in the habitat are provided along with a key to the species of Diplazium from Korea.

A Case of Gastrointestinal Stromal Tumor in a Child (소아에서 발생한 위장관 간질 종양 1예)

  • Yun, Kyung-Bin;Kim, Jae-Young;Ryu, Jae-Hong;Sul, Ji-Young;Kang, Dae-Young
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.10 no.1
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    • pp.71-75
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    • 2007
  • Gastrointestinal stromal tumors (GISTs) are the most common primary mesenchymal tumors of the digestive tract. They have been commonly observed in adults but have been rarely described in children. They arise typically from the intestinal wall and rarely in the mesentery, omentum, or retroperitoneum. GISTs originate from the interstitial cell of Cajal and are characterized by overexpression of the receptor tyrosine kinase c-kit. Up to 94% of these tumors express the CD117 on immunohistochemical stain. Surgery is the main modality of treatment for primary resectable GIST. Completely resectable GIST with low risk has excellent prognosis after primary surgical intervention, with over 90% of the 5-year survival. We report a case of 10-year-old girl presenting with an upper gastrointestinal bleeding caused by gastrointestinal stromal tumor.

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Anti-inflammatory and Wrinkle Improvement Effects of Peptides from Ginseng Berry Amino Acidic Complex (진생베리 아미노산 복합체로부터 분리한 펩타이드의 항염, 주름개선 효과)

  • Kang, Sang Moon;Park, Chung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.3
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    • pp.299-306
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    • 2019
  • Ginseng berry (GB) contains Ginsenoside Re and has anti-inflammatory and anti-wrinkle properties. In this study, TLC fractions 1, 2, and 4 of the ginseng berry amino acid complex were identified and analyzed by HPLC. And identified a peptide (AP-1) by LC/MASS analysis of fraction 1. The anti-inflammatory activity was confirmed by investigating the inhibitory effect of AP-1 on NO production. In addition, collagen synthesis using procollagen type I C-peptide (PIP) ELISA kit was 50% higher effective than that of the control group. From these results, the peptide isolated from ginseng berry amino acid complex is considered to have anti-inflammatory and anti-wrinkle effect, and may be useful as an anti-inflammatory and anti-aging cosmetic raw material.

Successful Postoperative Management of Gastrointestinal Stromal Tumor in a Dog

  • Lee, Ki-Sung;Kim, Keon;Yang, Chul-Ho;Suh, Guk-Hyun;Lee, Chang-Min
    • Journal of Veterinary Clinics
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    • v.38 no.4
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    • pp.184-188
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    • 2021
  • A 10-year-old spayed female Maltese with a history of vomiting and lethargy was referred to the hospital. Physical examination revealed dehydration and severe pain following abdominal palpation. A large mass was observed in the cranial abdomen through radiography and ultrasonography. Laparotomy was performed to find the origin of the mass. The mass was about 8 cm originating from the cecum and subsequently removed. Histopathologic evaluation revealed that the cecal mass was suspected to be a mesenchymal-derived tumor. Through immunohistochemistry, the mass was diagnosed as a gastrointestinal stromal tumor (GIST) based on the c-kit expression. Given its recurrence, postoperative preventive therapy was initiated with masitinib mesylate, which is a tyrosine kinase inhibitor. The animal did not show any side effects during the medication period. After 6 months of therapy, it was well controlled without any recurrence. In this case, we introduced a novel postoperative management of GIST using masitinib mesylate.

Java Android & C Android (Java 안드로이드 & C 안드로이드)

  • Lee, Ho-Suk
    • Proceedings of the Korean Information Science Society Conference
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    • 2012.06d
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    • pp.32-34
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    • 2012
  • 현재 Android 에서는 대부분 Java 언어로 응용 프로그램을 개발한다. 그러나 C 언어로 응용 프로그램을 개발해야 하는 경우도 많다. Android 에서 C 언어로 응용 프로그램을 개발하기 위해서는 NDK(Native Development Kit) 를 설치해서 사용해야 한다. Android 시스템에서 Linux 커널은 Android Runtime Java 프로세스만을 부팅한다. 그러나 만약 Android 에서 C 프로세스를 부팅한다면 C 언어로 직접 응용 프로그램을 개발할 수 있다. 본 논문은 Android 시스템에서 Android C 프로세스를 부팅시키는 간단한 방법을 제시한다. 그리고 적합한 C/C++ GUI(Graphic User Interface) 라이브러리와 응용 라이브러리를 확보하거나 또는 개발하여 설치한다면, 현재의 Java Android 를 C Android 로 변환시키게 된다.

Anti-Wrinkle Effect of Schizandra chinensis Baillon Fermented with Lactobacillus plantarum (오미자 유산균 발효물의 주름개선 효과)

  • Lee, Jung Hee;Kim, Jong Im;Choi, Hwa Jung;Lee, Jung Hyun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.4
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    • pp.365-371
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    • 2014
  • To identify new active anti-wrinkle ingredients, this study investigated the anti-wrinkle effects of Schizandra chinensis Baillon fermented with Lactobacillus plantarum (SCF) by assessment of cytotoxicity of human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1) inhibition and elastase inhibition. S. chinensis was fermented with L. rhamnosus for 1 day at $37^{\circ}C$. The cytotoxicity of SCF was evaluated by a cytopathic effect reduction method. Effects on collagen biosynthesis and matrix metalloproteinase-I (MMP-1) of SCF were evaluated by previous reported method using procollagen type-IC peptide EIA kit and Matrix Metalloproteinase-1 Biotrack activity Assay Kit, respectively. Elastase inhibition assay was conducted by reaction of enzyme and substrate using N-Suc-$(Ala)_3$-nitroanilide as the substrate. As the results, SCF didn't show cytotoxicity against human dermal fibroblast at concentration of $100{\mu}g/mL$. Also, SCF was increased collagen synthesis and showed inhibitory effect of MMP-1 (p < 0.05). In the elastase inhibition assay, the $IC_{50}$ of SCF was $36.4{\mu}g/mL$. Therefore, our results indicated that SCF possesses anti-wrinkle effects and can be used practically for anti-wrinkle care of skin.

An Identification of Enterobacter sp. Isolated from Contaminated Ginseng and Inhibition Effect of Ginseng Saponin on Its Growth (오염된 인삼으로부터 분리된 Enterobacter sp.의 동정 및 인삼사포닌의 균 생육억제효과)

  • 곽이성;이종태;여운형
    • Journal of Food Hygiene and Safety
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    • v.17 no.1
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    • pp.26-30
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    • 2002
  • A bacterium isolated from contaminated white ginseng was indentified by using API kit and electron microscope. The isolate was determined as rod shaped bacterium having 0.6-1.0 ${\mu}{\textrm}{m}$ in diameter and 1.2-3.0 ${\mu}{\textrm}{m}$ in length. It had motility by flagellum. The isolate had $\beta$-galactosidase, arginine dihydrolase and omithin decarboxylase. It used citrate as sole carbon source but not produced H$_2$S. It also fermented glucose, manitol, sorbitol, rhamnose, sucrose, melibiose, arabinose and amygdalin. The isolate was identified as Enterobacter sp by the above API kit analysis and electron microscopy observation. Ginseng saponin was added to culture of Enterobacter sp. in order to investigate saponin's influence on its growth. The strain was incubated at 38$^{\circ}C$ for 3 days after addition of 0.05, 0.5, 2.0 and 4.0% (w/v) of saponin, respectively and the growth rates were investigated. The relative bacterial growth rates showed 75.0, 37.5, 7.5 and 0.5%, respectively, when compared with 100% of saponin non-added group. These results suggest that the growth of Enterobacter sp. is inhibited by saponin with the concentration dependency.

Development and Evaluation of an Immunochromatographic Assay for Screening Listeria spp. in Pork and Milk

  • Kim, Seong-Hee;Kim, Jin-Young;Han, Woong;Jung, Byeong-Yeal;Chuong, Pham-Due;Joo, Hae-Jin;Ba, Hoa-Van;Son, Won-Geun;Jee, Young-Heun;Yoon, Byoung-Su;Lee, Yong-Soon;Lim, Yoon-Kyu
    • Food Science and Biotechnology
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    • v.16 no.4
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    • pp.515-519
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    • 2007
  • Rapid immunochromatographic assay (ICA) kits were developed using flagella-specific monoclonal antibodies (MAbs) and rabbit polyclonal antibodies for screening Listeria spp. in food. The establishment of different formats, MAb 2B1 as capture antibody and MAb 7A3 or rabbit polyclonal antibodies as detector antibody, was compared. The 2 formats of the ICA kit were shown to have specific reactions with Listeria and no cross-reactivity with any of the non-Listeria including Escherichia coli O157:H7 and Salmonella enteritidis. The detection limits of the ICA kit using the combination of gold-labeled MAb 7A3 and MAb 2B1 showed $1{\times}10^5$ and $1{\times}10^6\;CFU/0.1\;mL$ at 22 and $30^{\circ}C$, respectively. The other format of the ICA kit using the combination of gold-labeled rabbit polyclonal antibodies and MAb 2B1 showed $1{\times}10^6\;CFU/0.1\;mL$ at $22^{\circ}C$ but weak signal at 30 culture. The format utilizing MAb was more sensitive than the one using polyclonal antibodies for capture antibody. Samples contaminated with L. monocytogenes 4b culture (9-10, 5-6, and 1-2 CFU/mL) on pork and pasteurized milk were confirmed as positive results. Current data suggests that this ICA kit is a rapid, simple and effective tool to screen for Listeria spp. in food.

Comparison of a PCR Kit and a Selective Medium to Detect Pathogenic Bacteria in Eggs (PCR Kit와 선택배지를 이용한 계란의 병원성세균 검출 비교 평가)

  • Kim, Dong-Ho;Yun, Hye-Jeong;Song, Hyun-Pa;Lim, Sang-Yong;Jo, Min-Ho;Jo, Cheo-Run
    • Food Science and Preservation
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    • v.16 no.6
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    • pp.965-970
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    • 2009
  • PCR technology has been widely used to detect and quantify microbial pathogens in foodstuffs, because the technique is rapid, sensitive, and selective. In this study, detection of contaminating pathogenic bacteria on shells of chicken eggs was performed using both a commercial multiplex polymerase chain reaction (PCR) kit and a viable count method employing a selective medium. The PCR kit was capable of detecting Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Listeria monocytogenes, Yersinia enterocolitica, Salmonella species, and Shigella species. Using the PCR method, five bacterial species were detected from 30 samples (33.3%) of 90 batches of eggs commercially available in a market. PCR products from B. cereus, S. aureus, L. monocytogenes, Y. enterocolitica, and E. coli O157:H7 were detected, and the numbers and frequencies of positive samples were 17 (18.8%), 12 (13.3%), 15 (16.6%), 16 (17.7%),and 4 (4.4%), respectively. None of any Salmonella species, C. jejuni, V. parahaemolyticus, or Shigella species was detected in this study. The results of PCR testing were confirmed using a typical viable count method employing a selective medium. We suggest that the multiplex polymerase chain reaction (mPCR) assay is a rapid and reliable method for detection of pathogenic bacteria contaminating eggs.

Isolation and Identification of GABA-producing Microorganism from Chungkookjang (GABA 함량이 높은 청국장을 발효하는 균주의 분리 및 동정)

  • Mann, So-Yon;Kim, Eun-Ah;Lee, Ga-Young;Kim, Ro-Ui;Hwang, Dae-Youn;Son, Hong-Joo;Kim, Dong-Seob
    • Journal of Life Science
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    • v.23 no.1
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    • pp.102-109
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    • 2013
  • To isolate GABA-producing microorganisms, 1,500 strains were isolated from different Chungkookjang samples and screened. From these strains, 20 were selected for further analyses based on a protease and slime-producing activity test. The MC 31 strain showed the highest GABA concentration in Chungkookjang and was used in this study. MC 31 was identified as Bacillus subtilis by an API 50CHB kit and 16S rDNA sequences analysis and named as B. subtilis MC 31. B. subtilis MC 31 showed exponential growth up to 12 hours at $37^{\circ}C$ in LB broth, and it reached a stationary phase after 24 to 36 hours of incubation. B. subtilis MC 31 showed maximum GABA content at 72 hours after incubation at $40^{\circ}C$.