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Comparison of a PCR Kit and a Selective Medium to Detect Pathogenic Bacteria in Eggs  

Kim, Dong-Ho (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute)
Yun, Hye-Jeong (Department of Animal Science and Biotechnology, Chungnam National University)
Song, Hyun-Pa (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute)
Lim, Sang-Yong (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute)
Jo, Min-Ho (Department of Radiation Biotechnology, Korea Atomic Energy Research Institute)
Jo, Cheo-Run (Department of Animal Science and Biotechnology, Chungnam National University)
Publication Information
Food Science and Preservation / v.16, no.6, 2009 , pp. 965-970 More about this Journal
Abstract
PCR technology has been widely used to detect and quantify microbial pathogens in foodstuffs, because the technique is rapid, sensitive, and selective. In this study, detection of contaminating pathogenic bacteria on shells of chicken eggs was performed using both a commercial multiplex polymerase chain reaction (PCR) kit and a viable count method employing a selective medium. The PCR kit was capable of detecting Campylobacter jejuni, Escherichia coli O157:H7, Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Listeria monocytogenes, Yersinia enterocolitica, Salmonella species, and Shigella species. Using the PCR method, five bacterial species were detected from 30 samples (33.3%) of 90 batches of eggs commercially available in a market. PCR products from B. cereus, S. aureus, L. monocytogenes, Y. enterocolitica, and E. coli O157:H7 were detected, and the numbers and frequencies of positive samples were 17 (18.8%), 12 (13.3%), 15 (16.6%), 16 (17.7%),and 4 (4.4%), respectively. None of any Salmonella species, C. jejuni, V. parahaemolyticus, or Shigella species was detected in this study. The results of PCR testing were confirmed using a typical viable count method employing a selective medium. We suggest that the multiplex polymerase chain reaction (mPCR) assay is a rapid and reliable method for detection of pathogenic bacteria contaminating eggs.
Keywords
egg; PCR; pathogenic bacteria; detection;
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