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http://dx.doi.org/10.5352/JLS.2013.23.1.102

Isolation and Identification of GABA-producing Microorganism from Chungkookjang  

Mann, So-Yon (Department of Food Science & Technology, Pusan National University)
Kim, Eun-Ah (Department of Food Science & Technology, Pusan National University)
Lee, Ga-Young (Department of Food Science & Technology, Pusan National University)
Kim, Ro-Ui (Department of Food Science & Technology, Pusan National University)
Hwang, Dae-Youn (Department of Biomaterial Science, Pusan National University)
Son, Hong-Joo (Department of Life Science & Environmental Biochemistry, Pusan National University)
Kim, Dong-Seob (Department of Food Science & Technology, Pusan National University)
Publication Information
Journal of Life Science / v.23, no.1, 2013 , pp. 102-109 More about this Journal
Abstract
To isolate GABA-producing microorganisms, 1,500 strains were isolated from different Chungkookjang samples and screened. From these strains, 20 were selected for further analyses based on a protease and slime-producing activity test. The MC 31 strain showed the highest GABA concentration in Chungkookjang and was used in this study. MC 31 was identified as Bacillus subtilis by an API 50CHB kit and 16S rDNA sequences analysis and named as B. subtilis MC 31. B. subtilis MC 31 showed exponential growth up to 12 hours at $37^{\circ}C$ in LB broth, and it reached a stationary phase after 24 to 36 hours of incubation. B. subtilis MC 31 showed maximum GABA content at 72 hours after incubation at $40^{\circ}C$.
Keywords
Isolation; identification; GABA; Chungkookjang;
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