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http://dx.doi.org/10.15230/SCSK.2014.40.4.365

Anti-Wrinkle Effect of Schizandra chinensis Baillon Fermented with Lactobacillus plantarum  

Lee, Jung Hee (Department of Beauty Science, Kwangju Women's University)
Kim, Jong Im (Department of Nursing, Jungwon University)
Choi, Hwa Jung (Department of Beauty Science, Kwangju Women's University)
Lee, Jung Hyun (Department of Beauty Art, Youngsan University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.4, 2014 , pp. 365-371 More about this Journal
Abstract
To identify new active anti-wrinkle ingredients, this study investigated the anti-wrinkle effects of Schizandra chinensis Baillon fermented with Lactobacillus plantarum (SCF) by assessment of cytotoxicity of human dermal fibroblast, collagen biosynthesis, matrix metalloproteinase-I (MMP-1) inhibition and elastase inhibition. S. chinensis was fermented with L. rhamnosus for 1 day at $37^{\circ}C$. The cytotoxicity of SCF was evaluated by a cytopathic effect reduction method. Effects on collagen biosynthesis and matrix metalloproteinase-I (MMP-1) of SCF were evaluated by previous reported method using procollagen type-IC peptide EIA kit and Matrix Metalloproteinase-1 Biotrack activity Assay Kit, respectively. Elastase inhibition assay was conducted by reaction of enzyme and substrate using N-Suc-$(Ala)_3$-nitroanilide as the substrate. As the results, SCF didn't show cytotoxicity against human dermal fibroblast at concentration of $100{\mu}g/mL$. Also, SCF was increased collagen synthesis and showed inhibitory effect of MMP-1 (p < 0.05). In the elastase inhibition assay, the $IC_{50}$ of SCF was $36.4{\mu}g/mL$. Therefore, our results indicated that SCF possesses anti-wrinkle effects and can be used practically for anti-wrinkle care of skin.
Keywords
Collagen biosynthesis; Matrix metalloproteinase-I; Schizandra chinensis Baillon fermented with Lactobacillus plantarum; Elastase; Anti-wrinkle;
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Times Cited By KSCI : 4  (Citation Analysis)
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