• Asian-Australasian Journal of Animal Sciences
• /
• 제15권4호
• /
• pp.555-564
• /
• 2002

Twenty Landrace and twenty Min piglets, with an average initial body weight of 22.4 kg, were randomly divided into 5 groups with 4 animals per group, within each of the breeds. The piglets were housed in individual concrete pens. Each group of the piglets was fed one of 5 diets. The diets contained either 20% raw Argentine soybeans, 20% processed Argentine soybeans ($118^{\circ}C$ for 7.5 min.), 20% raw Chinese soybeans, 20% processed Chinese soybeans ($118^{\circ}C$ for 7.5 min.) or no soybean products (control diet). Faecal samples were collected on days 6, 7 and 8 of the treatment period. Digestibilities of dietary nutrients were determined with AIA (acid insoluble ash) as a marker. After a 17 day treatment, three piglets were killed from each of the groups. Tissue samples of small and large intestine for light and electron microscopy examination were taken immediately after the opening of abdomen. Then, the weight or size of relevant organs was measured. The results show that the digestibilities of dry matter (DM), crude protein (CP) and fat were higher in Min piglets than in Landrace piglets (p<0.05). The diets containing processed soybeans had a significant higher CP digestibility than the control diet and the diets containing raw soybeans (p<0.05). Landrace piglets had heavier and longer small intestines, heavier kidneys and a lighter spleen than Min piglets (p<0.05). The pancreas of the animals fed the diets containing processed soybeans was heavier than that of the animals fed control diet (p<0.05) and the diets containing raw soybeans. But, the differences between raw and processed soybean diets were not significant. A significant interaction (p<0.05) between diet and pig breed was observed in weight of the small intestine. The Landrace piglets increased the weight in their small intestine when they were fed the diets containing soybeans. In the light micrographs and electron scanning micrographs, it was found that the villi of small intestinal epithelium of animals (especially Landrace piglets) fed the diets containing raw Chinese soybeans were seriously damaged. The transmission electron micrograph showed that a lot of vesicles were located between the small intestinal microvilli of these piglets. The histological examination also indicated that the proportion of goblet cells in villi and crypts in the piglets consuming the control diet was significantly lower (p<0.01 and p<0.02, respectively) than those of the animals consuming the diets containing raw or processed soybeans.

• 한국수정란이식학회지
• /
• 제28권3호
• /
• pp.215-222
• /
• 2013

Bovine coat color is decided by the melanocortin receptor 1 (MC1R) genotype mutation and melanogenesis. Specially, in the various cattle breeds, dominant black coat color is expressed by dominant genotype of $E^D$, red or brown is expressed in the frame shift mutation of recessive homozygous e by base pair deletion and wild type of $E^+$ is expressed in various coat colors. However, not very well known about the effected of MC1R genotype mutation on the coat color through family lines in KBC. Therefore, this study were to investigate effect of MC1R genotype mutation on the coat color, and to suggest mating breed system in accordance with of MC1R genotype for increased on brindle coat color appearance. Parents (sire 2 heads and dam 3 heads) and offspring (total : 54 heads) from crossbreeding in KBC family line with the MC1R genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes expression verified by PCR-RFLP, and brindle coat color appearance to the family line of the cross mating breed from MC1R genotype pattern was determined. As a result, 4MC1R genetic variations, $E^+/E^+$ (sire 1), $E^+/e$ (sire 2 and dam 3), $E^+/e$ with 4 bands of 174, 207 and 328 bp (dam 1) and $E^+/e$ with 3 bands of 174, 207, 328 and 535 bp (dam 2) from parents (sire and dam) of KBC. However, 3 genetic variations, e/e (24%), $E^+/E^+$ (22%) and $E^+/e$ (56%) were identified in offspring. Also, brindle coat color expressrated was the e/e with the 0%, $E^+/E^+$ with 67% and $E^+/e$ with 77% from MC1R genotype in offspring on the cross mating of KBC. Furthermore, when the sire had $E^+/e$ genotype and the dam had $E^+/E^+$ with the 3 bands or $E^+/e$ genotype, and both had whole body-brindle coat color, 62% of the offspring had whole body-brindle coat color. Therefore, the seresults, the mating system from MC1R genotype patterns of the sires ($E^+/e$) and dams ($E^+/E^+$ with the 3 bands or $E^+/e$) with brindle coat color may have the highest whole body-brindle coat color expression in their offspring.

• 한국수정란이식학회지
• /
• 제28권3호
• /
• pp.207-213
• /
• 2013

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

• 한국가금학회지
• /
• 제9권2호
• /
• pp.63-101
• /
• 1982

육용계에서 가식육량을 효과적으로 추정할 수 있는 방법을 구명코자 White Cornish종과 S. C. W. Leghorn종. 그리고 이 두 품종의 상반교잡종을 각각 120수씩 계 480수를 공시하여 체중. 부위별 가식육 및 체형의 변화, 이들 형질에 발현된 잡종강세와 반성유전효과 및 화학적 조성분함량을 조사하고. 성장단계별 가식육량을 추정한 결과를 요약하면 다음과 같다. 1. W. Cornish종의 8주시 체중 및 사료요구율과 폐사율을 보면 각각 1,818.8$\pm$47.7g와 2.24 및 3.30%였고 S. C. W. Leghorn종에선 각각 667.8$\pm$28.9g와 3.28 및 2.50%였으며 잡종들의 능력은 이 두 품종의 중간정도로 정상발육을 하였다. 2. 체중에 있어서 계종간 차이는 2주령부터 유의성이 인정되었고. 성장곡선의 회귀방정식에서 성장속도를 표시하는 회귀계수률 보면 W. Cornish종( $b_{cc}$ =1,578)은 S. C. W. Leghorn종 ( $b_{LL}$ =1,378)에 비해 유의적으로 크고 교잡종들은 이 두 품종의 중간값( $b_{LC}$ =1,476, $b_{CL}$ =1,470)을 보인다. 가식육량의 경우도 계종간 증가양상이 체중의 경우와 비슷하였으나 육량증가의 회귀방정식에서 회귀계수의 크기가( $b_{LL}$ =1,563, $b_{LC}$ =1,651, b.$_{CL}$ =1,636, $b_{CC}$ =1,766) 체중에서 보다 컷는데 그 원인이 성장속도 차이때문인지를 구명하기 위해 주령(log x)과 체중에 대한 가식육량비율(log y) 간의 선형관계를 추정한 결과 각 계종의 회귀계수( $b_{LL=0.184}$, $b_{LC}$ =0.175, $b_{CL=0.165}$ 및 $b_{CC}$ =0.188)는 모두유의성이 인정되어 가식육의 증가속도는 체중의 증가속도보다 현저히 빠른 것으로 판명되었다. 3. 체중구성요소중 주령경과에 따라 그 중량비율이 증가되는 것은 우모, 복강지방, 흉부 및 퇴경부이고, 감소되는 것은 두부, 경부, 가식내장 및 부가식내장이며 방혈량, 각부, 익부 및 배부는 큰 변화를 보이지 않는다.

• 한국임상수의학회지
• /
• 제29권4호
• /
• pp.344-347
• /
• 2012

Bilateral convergent strabismus with exophthalmus(BCSE) is an eye disorder affecting many cattle breeds worldwide. BCSE is the most common in cattle at various types of strabismus. Divergent strabismus is of relatively low incidence than convergent strabismus. This report is the to shown a case of convergent and divergent strabismus with unilateral in the exophthalmus in a heifer Holstein cow. A female Holstein calf born with congenital progressive divergent strabismus in right eye and convergent strabismus in left eye was tested. Ocular and blood examination, and activity were checked from the first week, 8 month, and 16 month after birth. The ocular examination includes general inspection, fixation reflex and menace response. The general inspection is checking the degree of deviation of both eyes from the normal visual axis, which was determined by the amount of sclera permanently visible in the temporal corner of the eye. The stage is divided into 4 stages depending on the degree of deviation. The right eye shown in stage 4 continues after birth and the left eye shown stage 2, stage 3 and stage 4 at 1 week, 8 month and 16 month after birth, respectively. In fixation reflex and Menace response, both eye balls showed a normal response at 1 week, 8 month and 16 month after birth. Blood count and serum chemistry test were performed, but a specific factor was not detected outside the reference range.

• 한국임상수의학회지
• /
• 제29권4호
• /
• pp.271-276
• /
• 2012

사람에 연구에 따르면 심부전 진단에 널리 사용되는 NT-proBNP농도는 비만, 비만 관련인자들과 밀접한 상관 관계가 있는 것으로 알려져 있다. 그러나 이러한 상관관계는 아직까지 수의분야에서는 입증된 바가 없다. 따라서 본 연구에서는 다양한 정도의 이첨판 패쇄부전증(CMVI)에 이환된 73마리 개 집단에서 NT-proBNP와 비만 및 비만 관련인자들간의 상호관계를 조사하였다. 절식시 혈당 농도, 지방관련 인자(i.e., total triglycerides [TG], total cholesterol [TC], high-density lipoprotein cholesterol [HDL-C], low-density lipoprotein cholesterol [LDL-C]), fructosamine, insulin 및 NT-proBNP 농도를 측정하였다. Insulin/glucose ratio 역시 계산하였다. NT-proBNP 농도는 CMVI에 의한 심부전의 정도에 따라 농도변화가 관찰되었으며, body condition scores (BCS), insulin 및 fructosamine 농도와도 관련이 있었다. 또한 본 연구를 통해 비만과 혈중 NT-proBNP농도 간에 상관관계 역시 확인되었다. 또한 NT-proBNP농도는 비만과 관련 있는 인자인 fructosamine과 insulin농도와도 관련이 있었다. 본 연구는 개에서 NT-proBNP농도와 비만 정도와 상관관계를 입증한 첫 보고이다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제7권2호
• /
• pp.209-219
• /
• 2003

An experiment was initiated to evaluate hybrid vigour in twelve F$_1$ hybrids and seven three-way crosses of multivoltine ${\times}$ bivoltine silkworm between newly evolved multivoltine breed BL67 with productive bivoltine CSR breeds and hybrids. Analysis of variances computed for different characters among F$_1$hybrids and three-way crosses showed highly significant differences among them indicating presence of both additive and non-additive gene actions for the expression of these characters. Among twelve F$_1$ hybrids, two F$_1$ hybrids viz., BL67${\times}$CSR$_4$and BL67${\times}$CSR$_{5}$ have expressed significant heterosis for nine characters and two hybrids viz., BL67${\times}$NB$_4$D$_2$and PM${\times}$NB$_4$D$_2$ for eight characters whereas out of nine three-way crosses, three hybrids viz., BL67${\times}$ (CSR$_3$${\times}$CSR$_{6}$), BL67${\times}$(CSR$_{16}$${\times}$CRS$_{17}$) and BL67${\times}$(CSR$_{18}$ ${\times}$CSR$_{19}$) expressed significant heterosis for eight characters. In the present study, it is observed that the cocoons of two hybrids viz., BL67${\times}$CSR$_4$and BL67${\times}$CSR$_{19}$ were found to be uniform as these hybrids showed lowest CV％ (5.35 and 5.38) among twelve F$_1$ hybrids and seven three way crosses between multivoltine ${\times}$ bivoltine hybrids. Four F$_1$ hybrids viz., BL67${\times}$CSR$_4$, BL67${\times}$CSR$_{5}$, BL67${\times}$ (CSR$_3$${\times}$CSR$_{6}$), BL67${\times}$(CSR$_{16}$${\times}$CRS$_{17}$) and BL67${\times}$(CSR$_{18}$ ${\times}$CSR$_{19}$) showed superiority in expressing hybrid vigour and are considered as best heterotic hybrids for commercial exploitation.ion.ation.ion.ation.ion.

• 한국축산식품학회지
• /
• 제27권2호
• /
• pp.150-156
• /
• 2007

본 연구는 서로 다른 상염색체에 위치하고 있는 초위성체 유전 표지를 활용한 한국재래돼지 집단의 개체 식별시스템 설정을 위해 수행되었다. 공시재료는 4품종에서 총 446두가 사용되었으며 13종의 좌위에 대한 개체별 유전자형을 분석하였다. 이들 13종에서 출현된 이형접합도는 0.286-0.686였으며 marker 다형성 정보량은 0.399-0.796로 나타났다. 한국 재래돼지 집단에서 나타난 대립 유전자 발현 특성은 다른 대조 품종 집단과 매우 상이한 결과를 나타냈다. S0228좌위는 전체 8종의 대립유전자가 나타난 가운데 다른 3품종에서는 발현이 되지 않은 235 대립유전자가 한국 재래돼지 집단에서만 발현이 되었다. 5종의 초위성체 유전 표지를 활용할 경우 누적 개체 식별력은 99.999%를 나타냈으며 두 마리의 서로 다른 개체가 서로 같은 유전자형을 가질 짝확률은 $0.36{\times}10^{-9}$으로 추정되었다. 따라서 10종의 선정된 유전 표지는 한국재래돼지 집단에서 적정 신뢰도를 제공할 수 있는 개체 식별 시스템을 설정할 수 있을 것으로 생각된다.

• 한국축산식품학회지
• /
• 제27권3호
• /
• pp.382-386
• /
• 2007

본 연구는 비육돈에서 지방세포지방산결합단백질(A-FABP) 유전자의 유전자형과 도체형질의 상관관계를 조사하였다. 유전자 A-FABP의 프로모터 영역에 대해 HinfI-RFLP를 이용하여 유전자형을 조사한 결과 두 가지 유전자형(-406T/T T/C)들이 발견되었고 C/C는 발견되지 않았다. 유전자형 중 -406C/C 동형접합자가 발견되지 않는 이유는 비육돈 생산에 관여하는 돼지 품종들 중에서 Duroc 품종만이 이 유전자에 대한 고도의 다형성을 나타내기 때문으로 추정되었다. 유전자형 -406C/-를 나타내는 돼지들은 근내지방과 도체중이 통계적으로 유의적인 증가함을 나타내었으나 (p<0.05), 그 외의 도체형질들(조지방, 육색, 조직감, 수분 삼출도, 근육분리도)은 유의한 차이를 나타내지 않았다 (p>0.05). 본 연구를 통해 돼지 A-FABP 유전자의 -406C/- 유전자형이 근내지방에 대한 유용한 분자 마커가 될 수 있을 뿐만 아니라 다형성이 비육돈의 근내지방에 대한 유용한 분자 마커가 될 수 있으며, 특히 Duroc 품종을 최종 종모돈으로 이용할 경우 이 유전자형을 보유한 돼지를 이용하는 분자육종체계에서 marbling이 잘 된 돼지의 생산을 통한 육질 개선에 기여할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권12호
• /
• pp.1747-1753
• /
• 2002

The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and $30^{\circ}C$, respectively. Rigor values (R-values; $R_248$, $R_250$, and $R_258$), pH, muscle temperature, glycogen content, $\mu$- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower $\mu$-calpain activity than Holstein beef (p<0.05). The $30^{\circ}C$ treatment maintained muscle temperature of $30^{\circ}C$ until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The $16^{\circ}C$ had higher (p<0.05) muscle temperature at 3 h PM than the $2^{\circ}C$, but no difference in all other traits was observed between the $2^{\circ}C$ and the $16^{\circ}C$. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, $R_258$ had higher correlations with other metabolic traits than those of $R_248$ and $R_250$. These data suggest that early PM metabolic rate, $\mu$-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and $\mu$-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.