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http://dx.doi.org/10.5851/kosfa.2007.27.3.382

The Association between A-FABP Promoter Genotype and Carcass Traits in Pigs  

Han, Sang-Hyun (National Institute of Subtropical Agriculture, RDA)
Cho, In-Cheol (National Institute of Subtropical Agriculture, RDA)
Lee, Chong-Eon (National Institute of Subtropical Agriculture, RDA)
Ko, Moon-Suck (National Institute of Subtropical Agriculture, RDA)
Seong, Pil-Nam (National Institute of Animal Science, RDA)
Park, Beom-Young (National Institute of Animal Science, RDA)
Publication Information
Food Science of Animal Resources / v.27, no.3, 2007 , pp. 382-386 More about this Journal
Abstract
The association between the promoter region genotypes of the porcine adipocyte fatty acid binding protein (A-FABP) gene and carcass traits in commercial pigs was examined. Interestingly, only two different genotypes (-406T/T and T/C) for the A-FABP gene were detected using Hinf-RFLP for the substitution mutation T-406C in the A-FABP promoter in commercial pigs, and no -406C/C homozygotes were detected. The reason for the lack of the -406 C/C genotype is due to the breeding system in which only Duroc, which has a high frequency of -406C/- in this locus among the three breeds involved in commercial pig production, is typically used as a terminal sire. The pigs containing the genotype -406C/- were significantly associated with an increase in intramuscula. fat content and carcass weight (p<0.05), but there was no association with the other carcass traits tested (fat composition, color score, texture score, moisture, and separation score between muscles). This study suggests that the -406C/- genotype of the porcine A-FABP gene may not only be a useful molecular marker for intramuscular fat, but may also contribute to the improvement of meat quality by the production of well-marbled pigs by breeding animals containing this genotype, especially Duroc, as a terminal sire for commercial pigs.
Keywords
A-FABP; association; carcass trait; genotype; intramuscular fat;
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1 Gerbens, F., Jansen, A., van Erp, A. J., Harders, F., Meuwissen, T. H., Rettenberger, G., Veerkarnp, J. H., and te Pas, M. F. (1998) The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs. Mamm. Genome 9, 1022-1026   DOI
2 Kim J. H., Park, E. W., Park, J. J., Choi, B. W., Kim, T H., Seo, B. Y, Cheong, I. C., Lim, H. T, Oh, S. J., Lee, J. G., and Jeon, G. T (2005) Detection of novel mutations in the FABP3 promoter region and association analysis with intramuscular fat content in pigs. J. Amin. Sci. Technol. 47, 1-10
3 Miura, S., Tsunoda, N., Ikeda, S., Kai, Y., Cooke, D. W., Lane, M. D., and Ezaki, O. (2004) Nuclear factor lregulates adipose tissue-specific expression in the mouse GLUT4 gene. Biochem. Biophys. Res. Commun. 325, 812-818   DOI   ScienceOn
4 Marklund, L., Nystrom, P., Stem, S., Andersson-Eklund, L., and Andersson, L. (1999) Confirmed quantitative trait loci for fatness and growth on pig chromosome 4. Heredity 82, 134-141   DOI
5 Jones, K. A., Kadonaga, J. T, Rosenfeld, P. J., Kelly, T. J., and Tjian, R. (1987) A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. Cell 48, 79-89   DOI   ScienceOn
6 De Vol, D. L. E., McKeith, F. K., Bechtel, P. J., Novakofski, J., Shanks, R. D., and Carr, T. R. (1988) Variation in composition and palatability traits and relationships between muscle characteristics and palatability in a random sample of pork carcasses. J. Anim. Sci. 66, 385-395   DOI
7 Ovilo, C., Oliver, A., Noguera, J. L., Clop, A., Barrangan, C., Varona, L., Rodriguez, C., Taro, M., Sanchez, A., Perez-Enciso, M., and Silio, L. (2002) Test for positional candidate genes for body composition on pig chromosome 6. Genet. Sel. Evol. 34, 465-479   DOI   ScienceOn
8 SAS (1997) SAS/STAT Software for PC. User's Guide, SAS Institue Inc., Cary, NC, USA
9 Sato, S., Oyamada, Y., Atsuji, K., Nade, T., Sato, S-I., Kobayashi, E., Mistuhashi, T., Nirasawa, K., Komatsuda, A., Saito, Y., Terai, S., Hayashi, T., and Sugimoto, Y. (2003) Quantitative trait loci analysis for growth and carcass traits in a Meishan$\times$Duroc $F_{2}$ resource population. J. Anim. Sci. 81, 2938-2949
10 Scheepers, A., Doege, H., Joost, H. G., and Schurrnann, A. (2001) Mouse GLUT8: genomic organization and regulation of expression in 3T3-L1 adipocytes by glucose. Biochem. Biophys. Res. Commun. 288, 969-974   DOI   ScienceOn
11 Han, S. H., Cho, I. C., Lee, C. E., Kim, H. S., Lee, J. G., Jeon, J. T., Oh, M. Y, and Ko, M. S. (2006) Association between the polymorphism in the promoter region of porcine A-FABP gene and growth traits in Duroc pigs. J. Anim. Sci. Technol. 48, 21-28   DOI   ScienceOn
12 Cooke, D. W. and Lane, M. D. (1999) Transcription factor NFl mediates repression of the GLUT4 promoter by cyclic-AMP. Biochem. Biophys. Res. Commun. 260, 600-604   DOI   ScienceOn
13 Gerbens, F., Verburg, F. J., van Moerkerk, H. T., Engel, B., Buist, W., Veerkamp, J. H., and te Pas, M. F. (2001) Associations of heart and adipocyte fatty acid-binding protein gene expression with intramuscular fat content in pigs. J. Anim. Sci. 79, 347-354
14 Singh, M. V. and Ntambi, J. M. (1998) Nuclear factor 1 is essential for the expression of stearoyl-CoA desaturase 1 gene during preadipocyte differentiation. Biochim. Biophys. Acta 1398, 148-156   DOI   PUBMED   ScienceOn
15 Gerbens, F., de Koning, D. J., Harders, F. L., Meuwissen, T. H., Janss, L. L., Groenen, M. A., Veerkamp, J. H., van Arendonk, J. A., and te Pas, M. F. (2000) The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs. J. Anim. Sci. 78, 552-559
16 Gerbens, F., van Erp, A. J., Harders, F. L., Verburg, F. J., Meuwissen, T. H., Veerkamp, J. H., and te Pas, M. F. (1999) Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. J. Anim. Sci. 77, 846-852
17 Urban, T., Mikolasova, R., Kuciel, J., Ernst, M., and Ingr, I. (2002) A study of associations of the H-FABP genotypes with fat and meat production of pigs. J. Appl. Genet. 43, 505-509   PUBMED
18 Andersson, L., Haley, C. S., Ellergren, H., Knott, S. A., Johansson, M., Andersson, K., Andersson-Eklund, L., EdforLilja, I., Fredholm, M., Hansson, I., Hakansson, J., and Lundstrom, K. (1994) Genetic mapping of quantitative loci for growth and fatness in pigs. Science 263, 1771-1774   DOI
19 Graves, R. A., Tontonoz, P., Ross, S. R., and Spiegelman, B. M. 1991. Identification of a potent adipocyte-specific enhancer: involvement of an NF-1-like factor. Genes Dev. 5, 428-437   DOI   ScienceOn
20 Cho, I. C., Choi, Y. L., Ko, M. S., Kim, H. S., Lee, J. G., Jeon, J. T., and Han, S. H. (2005) Association between MSTN gene polymorphism and growth traits in Landrace pigs. J. Anim. Sci. Technol. 47, 159-166   DOI   ScienceOn
21 Nechtelberger, D., Pires, V., Soolknet, J., Stur, I., Brem, G., Mueller, M., and Mueller, S. (2001) Intramuscular fat content and genetic variants at fatty acid-binding protein loci in Austrian pigs. J. Anim. Sci. 79, 2798-2804