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http://dx.doi.org/10.12750/JET.2013.28.3.207

A Parentage Test using Indel, Microsatellite Markers and Genotypes of MC1R in the Jeju Black Cattle Population  

Han, Sang Hyun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Cho, Sang-Rae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Cho, In-Cheol (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Cho, Won-Mo (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Kim, Sang-Geum (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Yang, Sung-Nyun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Kang, Yong-Jun (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Park, Yong-Sang (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Kim, Young-Hoon (Institute for Livestock Promotion)
Park, Se-Phil (Department of Animal Biotechnology, Jeju National University)
Kim, Eun-Young (Mirae Biotech. Co./Jeju National University Stem Cell Research Center)
Lee, Sung-Soo (National Institute of Animal Science, RDA)
Ko, Moon-Suck (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Publication Information
Journal of Embryo Transfer / v.28, no.3, 2013 , pp. 207-213 More about this Journal
Abstract
This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of $4.1202{\times}10^{-4}$ than those previously recommended by International Society of Animal Genetics (ISAG) of $5.000{\times}10^{-4}$ for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of $2.679{\times}10^{-5}$. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.
Keywords
molecular marker; parentage; microsatellite; indel; Jeju Black cattle;
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Times Cited By KSCI : 6  (Citation Analysis)
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