• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.107-117
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• 2020

Flower industry is now growing due to the development of economy in many countries. Simultaneously, needs from consumers in flower market are varied widely. To satisfy the needs from consumers and deal with a variety of diseases from a lots of pathogens as well as climate change, new elite flower cultivars should be released in flower market. For this purpose, conventional and biotechnological techniques can be employed to make good cultivar. Therefore, this review describes the general overview of flower breeding techniques including cross-hybridization, mutation breeding and genetic transformation systems. Also, breeding systems for ornamentals derived from plant tissue culture such as embryo culture, in vitro fertilization, ovary/ovule culture and haploid production were reviewed. Furthermore, in this study recent development of the generation of new flower cultivars using marker-assisted breeding, plant transformation including particle bombardment and Agrobacterium tumefaciens as well as genome-editing technology were described. This review will be contributed to the development and releasement of new flower cultivars with horticulturally useful traits in the future.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.3
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• pp.35-46
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• 2023

It was in the 1982 that artificial seed production research for olive flounder (Paralichthys olivaceus) farming was first conducted in Korea (Currently, National Institute of Fisheries Science, Fish Breeding Research Center). In 1985, fertilized eggs were obtained from natural olive flounder adapted to land tanks, and artificial seed production technology was established and fertilized eggs were distributed. In the late 1980s, halibut aquaculture began to prosper in land-based tank farming in Jeju Island and Busan's Gijang region, where water temperatures are relatively high in winter. Currently, aquaculture is being carried out all over the country, centering on Jeju Island and Wando, Jeollanam-do. However, olive flounder farming, which started with a small group in the 1980s, reduced genetic diversity through inbreeding over generations, resulting in side effects such as slow growth, reduced resistance to disease and environmental conditions. In order to solve these genetic problems of farmed olive flounder in Korea, the Fish Breeding Research Center of the National Institute of Fisheries Science introduced a wild-caught parent fish group to the existing aquaculture group from 2003 to 2004. Genetic diversity was secured and KingNupchi with fast growth and improved body shape was developed. In this study, the current status of breeding technology development of olive flounder, a major aquaculture breed in Korea, is reviewed and future research directions are suggested.

• Korean Journal of Veterinary Research
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• v.15 no.2
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• pp.215-222
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• 1975

Infertility and breeding status of Korean cows which are reared in a extensive alpine ranches of Daegwanryeong area was investigated. The results obtained were summarized as follows, 1. Infertility frequency of the cows was 25.9% against a total examined number of 675 heads, and was 17.6% against a total rearing number. 2. The distributions of infertile cows 44.6% of Granular vaginitis-like syndrome, 36% of repeat breeder, 8.6% of symptom of ovary, 6.8% of symptom of uterus and 4% of other symptoms. 3. According to its age were found by 51.4% upon 2 years of age, 30.3% upon 3 years of age, 11.4% upon 4 years of age and 6.9% upon S years of age. 4. Breeding status of Korean cows showed 74.1% of conception rate in the cases of pre-treatment, while breeding status increased markedely in the cases of post-treatment by the 85.8%. 5. The conception rate by the artificial insemination mainly on the first mating was 36.1%. on the second mating 23.0% and on the third mating 18.0%. Total conception rate between first and third mating was 77.1%. 6. The therapeutic effect by treatment for granular vaginitis-like syndrome was 87.1%.

• Asian Journal of Turfgrass Science
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• v.11 no.1
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• pp.73-85
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• 1997

Although zoysia grass (Zoysia spp.) has a lot of excellent chracteristics as warm-season grass, it have been limited in use due to slow establishment, low seed production, poor shade tolerance and other factors. Breeding trials have been continued from 1900's, much attentions have been paid especially in U.S.A., Korea and Japan recently. In U.S.A., more than 24 varieties had been evaluated at National Turfgrass Evaluation Program(NTEP) from 1991 to 1995 and some were regsistered as commercial. After the 6th International Turfgrass Research Conference at Japan in 1989, Japan Turfgrass In-corporation (JTI) sponsored by private companies and government carried out breeding programs for pest, salt, and shade tolerant and herbicide resistant varieties. JTI also has been trying to im-prove vigor and breed evergreen zoysia Korean breeders collected germplasms since 1960's. After USDA breeders came to Korean penesula in 1982, Korean breeders joined with USDA zoysia breeding project for several years. Many interspecific hybrids and natural selected varieties were breeded that period both in U.S.A and Korea. Breeding objectives were to extend green color period, improve leaf quality and density, and better leaf color at dormant stage. Since 1990's, zoysia grass breeding trials are getting more diverse in many points such as random amplified polymorphic DNA (RAPD) assay for ecotype identification. The objectives of this study are to evaluate germplasms in Korea, and also review the present status and future prospect in zoysia grass breeding in the world.

• Ocean and Polar Research
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• v.31 no.3
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• pp.277-282
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• 2009

Saunders' gulls (Laurs saundersi) which are listed as globally vulnerable species on the IUCN Red List are distributed in East Asia and breed mainly in China and Korea. Their population is estimated to be between 7,100 to 9,600. Songdo reclaimed lands in the western coast of South Korea are the main breeding sites for these species. However, most of these breeding sites have been seriously disturbed in the last several years because of new urban development constructions. This study was conducted to provide baseline data on the breeding ecology of Saunders' gulls. Since 1998, the breeding population has rapidly increased in South Korea, with the number of nests rising from 85 nests in 2000 to 185 nests in 2005. Egg volume is more variable than breadth or length. Clutch size, hatching and breeding success were $2.9{\pm}0.81$ (n=182, mean$\pm$sd.), 48.07% (75 hatched egg/156 eggs) and 29.08% (155 fledglings/533 eggs), respectively. Factors affecting breeding failure were predation, rainfall, starvation and supernormal clutches. These results are provided along with other cited literature to argue for conservation of Saunders' gulls.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.193-206
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• 2023

Orchidaceae species account for one-tenth of all angiosperms including more than 30,000 species having significant ecological, evolutionary, and economic importance. Despite Orchidaceae being one of the largest families among flowering plants, crucial cytogenetic information for studying species diversification, inferring phylogenetic relationships, and designing efficient breeding strategies is lacking, except for 10% or less of orchid species cases involving mostly chromosome number or karyotype analysis. Also, only approximately 1.5% of the identified orchid species from less than a hundred genera have genome size data that provide crucial information for breeders and molecular geneticists. Various molecular cytogenetic techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), have been developed for determining ploidy levels, analyzing karyotypes, and evaluating hybridity, in several ornamental crops including orchids. The estimation of genome size and the determination of nuclear DNA content using flow cytometry have also been employed in some Orchidaceae subfamilies. These different techniques have played an important role in supplementing beneficial knowledge for effective plant breeding programs and other related plant research. This review focused on orchid breeding summarizes the status of current cytogenetic tools in terms of background, advancements, different techniques, significant findings, and research challenges. Principal roles and applications of cytogenetics in orchid breeding as well as different ploidy level determination methods crucial for breeding are also discussed.

• Journal of Plant Biotechnology
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• v.45 no.4
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• pp.299-305
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• 2018

Although new plant breeding technologies facilitate efficient plant breeding without introducing a transgene, they are creating indistinct boundaries in the regulation of genetically modified organisms (GMOs). The rapid advancement in plant breeding by genome-editing requires the establishment of a new global policy for the new biotechnology, while filling the gap between process-based and product-based GMO in terms of regulations. In this study recent developments in producing major crops using new plant breeding technologies were reviewed, and a regulatory model that takes into account the various methodologies to achieve genetic modifications as well as the resulting types of mutation were proposed. Moreover, the communication process were discussed in order to understand consumers' current situation and problems of new plant breeding technology, establish social acceptance well, and understand consumers' disputes such as GMO crops.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.5
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• pp.598-602
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• 2004

The Tongcheng pig breed is a famous Chinese indigenous breed. The Ministry of Agriculture of China has filed it as 1 of 19 national key conservation breeds selected from more than 100 Chinese indigenous pig breeds in 2000. In order to improve the reproductive performance, it has been intensively selected to increase the litter size for about 10 years. The population randomly sampled from conservation nucleus of eight families in the Tongcheng pigs was genotyped for identification of their estrogen receptor locus polymorphisms with the PCR-RFLPs method. Only AB heterozygotes and BB homozygotes were detected, and $X^2$ test demonstrated that the locus was in disequilibrium at a significant level (p<0.05). In the present paper, the litter sizes in different parities were regarded as different traits. Holistic status of other unspecific and unidentified genes was estimated by using the statistical methods. Coefficients of kurtosis and skewness showed that the litter size still presented segregating characteristic in the 2nd, 5th, 7th, 8th and 9th parities. Analysis of homogeneity of variance between families confirmed the results for the 5th, 7th and 8th parities. The heritability of litter size for the 1st to 10th parities was estimated with paternal half-sib model and individual estimated breeding values (EBVs) were evaluated by a single trait animal model as well. We found that the averages of EBVs for litter size in each parity did not differ significantly between genotypes, despite the significant difference for original phenotype records in the 3rd, 4th and 5th parities (p<0.05 or p<0.01). The results may be explained by the deduction that the polymorphisms of ESR locus are no longer the important genetic base of litter size variation when the frequency of allele B accumulated in the experience of selection procedure, and further conferring that there exist special genes associated with litter size in the recent Tongcheng pigs population can be made.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.16
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• pp.35-45
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• 1974

• Proceedings of the Botanical Society of Korea Conference
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• 1995.07a
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• pp.29-56
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• 1995