• 제목/요약/키워드: breeding efficiency

검색결과 345건 처리시간 0.023초

SSR Analysis of Genetic Diversity and Nitrogen Use Efficiency Traits in Rice

  • Kim, Myung Ki;Oh, Myeong Kyu;Lee, Jeong Heui;Kim, Yeon Gyu;Lee, Young Tae;Kim, Kwang Ho;Ahn, Sang Nag
    • 한국육종학회지
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    • 제40권2호
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    • pp.119-127
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    • 2008
  • A total of 41 microsatellite markers were used with 29 genotypes to examine the relationship between SSR polymorphisms and N-use efficiency related traits with a goal to identify the putative QTLs related to these traits. These primers yielded a total of 183 alleles (average 4.46 alleles per primer), and polymorphism information content (PIC) values of the SSRs ranged from 0.119 to 0.805 with mean value of 0.425. Correlation coefficients were obtained among the four N-use efficiency traits in the 34 accessions and significant positive correlations of relative ratios between grain yield and harvest index (r=0.3404) and total dry matter (r=0.7976), while N uptake showed a moderate level of correlation with the ratios of the grain yield and total dry matter, respectively. 36.5% (15/41) SSR markers were monomorphic among the 25 japonica accessions out of the 29 accessions. Association between SSR genotypes and phenotypic performances from the total (29) or japonica (25) accessions was tested based on a single point analysis. Three putative QTL regions were detected for the ratio of grain yield. These include the chromosomal region containing the RM283 locus on chromosome 1 and RM25 on chromosome 8 (all and japonica accessions) and the region with the SSR marker, RM206 on chromosome 11 (the japonica accessions). For the total dry matter ratio, two chromosomal regions were identified as the putative QTL region. One is the region with the SSR marker, RM162 on chromosome 6 (all and japonica accessions) and the other was the one with the SSR marker RM25 on chromosome 8 (the japonica accessions). Among these markers, RM25 showed associations with both traits.

국내 양돈장의 사육 온도와 밀사율이 구강액 채취율에 미치는 영향 (The effect of temperature and breeding density of piggery on the collection of oral fluid in Korea)

  • 변현섭;김미화;권성애;한미나;한성태;장래훈;정윤수;김석현;전보영
    • 한국동물위생학회지
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    • 제44권4호
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    • pp.217-225
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    • 2021
  • We investigated the effect of temperature and stock density on the collection efficiency of oral fluid in the pig farm in Korea. Three pig farms with similar breeding environmental conditions were selected and four pens of each farm (total 12 pens) were tested for the collection efficiency of oral fluid from pigs. Collection rate was considered as significant when oral fluid was collected from 70% of pigs within a pen. In the case of growing pigs, when internal temperature of pig barn increased by one designated degree (5℃), the oral fluid collection rate significantly decreased by 24.7% (P<0.05). The collection rate of oral fluid also decreased by 7.1% (P<0.05) as the density rate increase by one designated degree (12.5%). It was estimated that the collection efficiency of oral fluid decreased when the internal temperature of pig barn was 30℃ or higher, or barn density is higher 25% or high. On the other hand, in the case of stall-housing sows, unlike growing pigs, there was no significant differences according to the temperature, so oral fluid collection was considered to be efficient even in hot season.

SNP 마커를 이용한 벼 흰잎마름병 저항성 선발 효율 증진 (Improvement of Selection Efficiency for Bacterial Blight Resistance Using SNP Marker in Rice)

  • 신운철;백소현;서춘순;강현중;김정곤;신문식;이강섭;한장호;김현순
    • Journal of Plant Biotechnology
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    • 제33권4호
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    • pp.309-313
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    • 2006
  • 본 연구는 흰잎마름병 K1 레이스에 감수성인 상주찰벼와 저항성인 HR13721-53-3-1-3-3-2-2를 인공교배하여 육성된 F2, F3를 재료로 하천 Kl 레이스에 대한 저항성 검정과 SNP 마커를 이용한 유전자형 분석 및 저항성과의 연관성을 분석하였다. Kl 레이스에 대한 저항성 검정 결과 $F_2,\;F_3$에서 각각 이론적 분리비인 3:1, 1:1의 분리비를 나타냈으며 SNP 마커를 이용한 유전자형 분석은 16PFXa1 primer를 이용하여 유전자를 증폭한 후 Eco RV 제한효소 처리하여 다형성을 분석하여 저항성 및 유전자형을 확인할 수 있었다. K1 레이스에 대한 저항성 검정과SNP마커를 이용한 유전자형의 연관분석 결과 저항성과 마커간에 연관성이 일치하였으며, 특히 SNP 마커를 이용한 유전자형 분석에서는 K1 레이스에 대한 저항성 검정에서 알 수 없었던 $F_2$ 개체가 동형접합체인지 이형접합체인지를 판별할 수 있어 저항성 품종 육종을 위한 선발 효율을 높일 수 있었다.

Transformation of Wheat Immature Embryos by Particle Bombardment

  • Wu Li-Min;Wei Yu-Ming;Zheng You-Liang
    • Journal of Plant Biotechnology
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    • 제7권2호
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    • pp.113-121
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    • 2005
  • The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to immature embryos of Chinese spring wheat (Triticum aestivum L.). Efficiency of DNA (uidA gene) delivery was assessed by transient GUS (${\beta}$-glucuronidase) expression in bombarded tissues. Of the parameters analyzed, acceleration pressure, bombardment distance, chamber vacuum pressure, bombardment times, osmotic conditioning of culture had a remarkable influence on transient gene expression. A bombardment procedure suitable for Chinese spring wheat cultivars was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues. The high efficiency made the system practical for wheat genetic transformation research and accelerating wheat breeding programs.

Translational gut microbiome research for strategies to improve beef cattle production sustainability and meat quality

  • Yasushi Mizoguchi;Le Luo Guan
    • Animal Bioscience
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    • 제37권2_spc호
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    • pp.346-359
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    • 2024
  • Advanced and innovative breeding and management of meat-producing animals are needed to address the global food security and sustainability challenges. Beef production is an important industry for securing animal protein resources in the world and meat quality significantly contributes to the economic values and human needs. Improvement of cattle feed efficiency has become an urgent task as it can lower the environmental burden of methane gas emissions and the reduce the consumption of human edible cereal grains. Cattle depend on their symbiotic microbiome and its activity in the rumen and gut to maintain growth and health. Recent developments in high-throughput omics analysis (metagenome, metatranscriptome, metabolome, metaproteome and so on) have made it possible to comprehensively analyze microbiome, hosts and their interactions and to define their roles in affecting cattle biology. In this review, we focus on the relationships among gut microbiome and beef meat quality, feed efficiency, methane emission as well as host genetics in beef cattle, aiming to determine the current knowledge gaps for the development of the strategies to improve the sustainability of beef production.

Estimation of Genetic Parameters for Four Reproduction Component Traits in Two Chinese Indigenous Pig Breeds

  • Zhu, M.J.;Ding, J.T.;Liu, B.;Yu, M.;Fan, B.;Li, C.C.;Zhao, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권8호
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    • pp.1109-1115
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    • 2008
  • The reproduction component traits are important components of sow efficiency. The objective of this study was to evaluate the phenotypic and genetic parameters of four reproduction component traits (age at puberty (AP), preweaning number dead (PND), weaning to service interval (WSI), and intra-individual SD in litter size (IISDLS)) of sows in two Chinese indigenous pig breeds. Available reproductive records including 22,591 piglets born from 2,054 litters by 574 Jiangquhai sows and 464 Meishan sows were used in this investigation. A set of mixed models and restricted maximum likelihood methodology were used for the multiple trait analyses of these traits. The results showed that the estimates of heritabilities (${\pm}$standard error) for AP, PND, WSI and IISDLS were $0.40{\pm}0.05$, $0.06{\pm}0.03$, $0.20{\pm}0.02$ and 0.09{\pm}0.03 in Jiangquhai sows, and $0.35{\pm}0.06$, $0.05{\pm}0.03$, $0.18{\pm}0.03$ and $0.10{\pm}0.04$ in Meishan sows, respectively. There was moderate genetic correlation between AP and WSI, while there were low genetic correlations between the other pairwise traits. The genetic correlations were positive for most of the pairwise traits, except for the one between AP and IISDLS. The results indicated that all traits except for AP were difficult to make genetic improvement by traditional selection methods due to low heritabilities and the favorable improvement of AP might result in unfavorable changes of IISDLS due to the trend of genetic antagonism.

액체배양을 이용한 단기 벼 형질전환 방법 (Rapid Agrobacterium-mediated genetic rice transformation method using liquid media)

  • 양대화;장안철;안일평;김해정;김동헌;이효연;서석철
    • Journal of Plant Biotechnology
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    • 제40권1호
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    • pp.37-42
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    • 2013
  • 본 연구는 종자로부터 유도된 캘러스를 아그로박테리움을 이용해 감염하여 고체배지에서 배양하는 기존의 방법과 달리, 형질전환에 소모되는 노동력과 시간, 비용을 단축하고자 감염과 공동배양, 균제거와 캘러스 선발까지 액체배양을 시도하였다. 성숙 종자로부터 유도된 캘러스를 바로 아그로박테리움으로 감염함으로써 조직배양으로 인한 체세포 변이의 발생을 최소화하고 감염부터 그 후 캘러스선발까지 총 4단계의 시간과 비용을 최소화하였으며, 감염된 캘러스로부터 재분화 시킴으로써 형질전환 식물체를 얻는 방법을 새롭게 수립하였다. 배양과정 중 감염과 공동배양 기간을 3일로 단축시킴으로써 캘러스의 스트레스를 최소화 하였고, PCR 분석을 통해 원하는 목표 유전자가 형질전환체에 안정적으로 도입이 되는 것도 확인할 수 있었다. 이러한 결과를 종합해 볼 때, 본 실험을 통해 얻어진 새로운 액체배양 방법은 우수한 농업적 형질을 가진 벼 품종 개발시 효율적으로 이용할 수 있을 것으로 생각된다.

Effect of Heifer Frame Score on Growth, Fertility, and Economics

  • Senturklu, S.;Landblom, D.G.;Perry, G.A.;Petry, T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권1호
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    • pp.69-78
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    • 2015
  • A non-traditional forage-based protocol was employed to evaluate replacement heifer growth, fertility, and economics between small frame (SF, 3.50; n = 50) and large frame (LF, 5.56; n = 50) heifers using three increasing gain growth phases. Preceding an 85 d growing-breeding period (Phase 3; P3) the heifers were managed as a common group for Phases 1 and 2 (P1 and P2). During P1, heifers grazed common fields of unharvested corn and corn residue (total digestible nutrients [TDN] 56%) with supplemental hay. For P2, heifers grazed early spring crested wheatgrass pasture (CWG; TDN 62%) that was followed by the final P3 drylot growing and breeding period (TDN 68%). Small frame heifers were lighter at the end of P1 in May and at the start of P3 breeding in August (p = 0.0002). Percent of mature body weight (BW) at the end of P1 (209 d) was 48.7% and 46.8%, respectively, for the SF and LF heifers and the percent pubertal was lower for SF than for LF heifers (18.0% vs 40.0%; p = 0.02). At breeding initiation (P3), the percentage of mature BW was 57.8 and 57.2 and the percentage pubertal was 90.0 and 96.0 (p = 0.07) for the SF and LF heifers, respectively; a 5-fold increase for SF heifers. Breeding cycle pregnancy on days 21, 42, and 63, and total percent pregnant did not differ (p>0.10). In drylot, SF heifer dry matter intake (DMI) was 20.1% less (p = 0.001) and feed cost/d was 20.3% lower (p = 0.001), but feed cost/kg of gain did not differ between SF and LF heifers (p = 0.41). Economically important live animal measurements for muscling were measured in May and at the end of the study in October. SF heifers had greater L. dorsi muscle area per unit of BW than LF heifers (p = 0.03). Small frame heifer value was lower at weaning (p = 0.005) and the non-pregnant ending heifer value was lower for SF heifers than for the LF heifers (p = 0.005). However, the total development cost was lower for SF heifers (p = 0.001) and the net cost per pregnant heifer, after accounting for the sale of non-pregnant heifers, was lower for SF heifers (p = 0.004). These data suggest that high breeding efficiency can be attained among March-April born SF and LF virgin heifers when transitioned to a more favorable May-June calving period through the strategic use of grazed and harvested forages resulting in a lower net cost per pregnant SF heifer.

육계사료에 항생제 첨가 효과와 경제성에 관한 조사연구 II (the additional effects of antibiotics for the chicken breeding feed and economical efficiency.)

  • 오세정;노순창;정태영
    • 한국가금학회지
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    • 제9권1호
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    • pp.17-33
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    • 1982
  • The purpose of this experiment is to clarify the additional effect of antibiotics for the chicken breeding feed. This experiment was carried out with 6,560 individuals which were collected from four farms, Dugil, Hyubhoi, Sanggog and Kumsan. The duration of this experiment was 6 months from July to December in 1981. 1. Body weight and body gain. (1) The range of the body gain was 1.91-7.60% The most effective sections was T$_3$, and the effect was T$_2$, T$_4$, T$_1$, T$\sub$5/ in order. (2) The environmental sanitation of the Sanggog farm was not so good. In this farm the body gain was severely occurred in T$_3$ and T$_2$ sections. Mere gain obtained from T$\sub$5/ section. (3) The environmental sanitation was good in Dugil, Hyubhoi and Kumsan. A little body gain was occurred in these farms, but differences were shown according to the place or breeds. 2. Amount of feed intake feed conversion ratio. (1) Amount of feed intake was increased 0.13-3.19%, and feed conversion ratio was improved 1.68-4.62% compared with control. T$_3$ section showed high percent and T$_4$ T$_1$, T$\sub$5/, Is, C in order. (2) The keeping method of the chicken breed in Hyubhoi was floor brooding. From Hyubhoi and Sanggog farms, the efficiency was increased 9.4-9.8%. (3) From Dugil and Kumsan, the rate of improvement was low. 3. Mortality : Mortality was merely decreased in Sanggog and Kumsan farms which the sanitary and fowl caring conditions had been had, by additional antibiotics. But there was no worthy effect. 4. The ratio of carcass and the degree of skin coloration. The additional antibiotics in feed had no effect on the ratio of carcass but had the influence on the yellowish skin coloration. Skin coloring was good in T$\sub$7/ section and T$_1$, T$\sub$6/, T$_4$, C, T$_3$, T$\sub$5/, T$_2$ in order. 5. Economical efficiency (1) The addition of the antibiotics in feed increased 3.76-10.95% of gross earning. The highest earning was occurred in T$_4$ section and T$_3$, T$_1$, T$_2$, T$\sub$5/, C sections in order. (2) The earning rate was influenced by the antibiotic products. (3) In Dugil and Kumsan farms, there was no difference in earning, however, in Hyubhoi and Sanggog farms there was wide difference in earning. Conclusionally, the addition of antibiotics in chicken breeding feed is necessary. Especially in bad condition of environmental sanitation and floor brooding, the effect of additional antibiotics is clear. Selection of antibiotics from various kinds, maker's confidence and quality problems are important.

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基于DEA模型的威海渔业生产效率评价研究 (The Evaluation Research of Weihai Fishery Production Efficiency Based on DEA Model)

  • 吴宜诺
    • Journal of East Asia Management
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    • 제3권1호
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    • pp.11-23
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    • 2022
  • Under the circumstances of China's slower economic growth, the first document ofthe central committee of the CPC continue to focus on "three agricultures" problems, agriculture play a basic role on China's economic. Since 2007, the first document directly stresses the important role of agricultural and fisheries every year. Central Government Working Report of 2015 also stresses that under the new normal of economy, it is important to improve quality and efficiency of agriculture. Agricultural focus going forward will be on improving capacity of competitiveness, innovation and sustainable development.The fishery as an important part of agriculture plays a vital role in the protection of national food security, the prosperity of the rural economy and the optimization of national food structure. However, the situation faced on accelerating the speed of Chinese fisheries is still grim. As an important fishery breeding city in my country, Weihai has achieved remarkable results in the development of fisheries. Based on the input-output indicators of Weihai City from 2010 to 2020, this article uses the DEA model method to conduct a comprehensive analysis of the factors affecting the fishery production efficiency in Weihai City. This paper calculates the two stages of comprehensive efficiency, pure technical efficiency and scale efficiency, and comprehensive compares the two stages. The research results show that: From 2010 to 2020, the average comprehensive technical efficiency of Weihai fishery was 0.928, the average scale efficiency was 0.963, and the average pure technical efficiency was 0.963. The comprehensive technical efficiency of Weihai fishery production showed an upward and downward trend, the pure technical efficiency showed a downward and then upward trend, and the pure technical efficiency showed a fluctuating trend.