Browse > Article

Transformation of Wheat Immature Embryos by Particle Bombardment  

Wu Li-Min (Triticeae Research Institute, Sichuan Agricultural University, Biotechnology College, Jilin Agricultural University)
Wei Yu-Ming (Triticeae Research Institute, Sichuan Agricultural University)
Zheng You-Liang (Triticeae Research Institute, Sichuan Agricultural University)
Publication Information
Journal of Plant Biotechnology / v.7, no.2, 2005 , pp. 113-121 More about this Journal
Abstract
The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to immature embryos of Chinese spring wheat (Triticum aestivum L.). Efficiency of DNA (uidA gene) delivery was assessed by transient GUS (${\beta}$-glucuronidase) expression in bombarded tissues. Of the parameters analyzed, acceleration pressure, bombardment distance, chamber vacuum pressure, bombardment times, osmotic conditioning of culture had a remarkable influence on transient gene expression. A bombardment procedure suitable for Chinese spring wheat cultivars was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues. The high efficiency made the system practical for wheat genetic transformation research and accelerating wheat breeding programs.
Keywords
Genetics transformation; Particle bombardment; Transient expression; Triticum aestivum L.;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M and Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5: 285-297   DOI   ScienceOn
2 Ortiz JPA, Reggiardo MI, Ravizzini RA, Altabe SG, Cervigni GDL, Spitteler MA, Morata MM, Elias FE, Vallejos RH (1996) Hygromycin resistance as an efficient selectable marker for wheat stable transformation . Plant Cell Rep 15: 877-881   DOI   ScienceOn
3 Perl A, Kless H, Blumenthal A, Galili G, Galunj E (1992) Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization. Mol Gen Genet 235: 279-284   DOI
4 Rasco-Gaunt S, Riley A, Barcelo P, Lazzer PA (1999) Analysis of particle bombardment parameters to optimize DNA delivery into wheat tissues. Plant Cell Prep 19: 118-127   DOI   ScienceOn
5 Rasco-Gaunt S, Riley A, Cannell M, Barcelo P, Lazzeri PA (2001) Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment. J Exp Bot 52: 865-874   DOI
6 Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, and Botterman J (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J. 6: 2519-2523
7 Viertel K, Schmid A, Iser M, Hess D (1998) Regeneration of german spring wheat varieties from embryogenic scutellar callus. J Plant Physiol 152: 167-172   DOI
8 Zheng Y-L, Lan X-J, Wei Y-M, Zhou Y-H, Liu D-C, Yang J-L, Van J (2002) Analysis on agronomic characters of new wheat variety chuannong 16. Journal of Sichuan Agricultural University 20: 189-192
9 Harvey A, Moisan L, Lindup S, Lonsdale D (1999) Wheat regenerated from scutellum callus as a source of material for transformation. Plant Cell Tiss Org Cult 57: 153-156   DOI   ScienceOn
10 Iser M, Fettig S, Scheyhing F, Viertel K, Hess D (1999) Genotype-dependent stable genetic transformation in german spring wheat varieties selected for high regeneration potential. J Plant Physiol 154: 509-516   DOI   ScienceOn
11 Leonie P, Steele SH, Barcelo P, Shewry PR, Lazzeri PA (2003) Transgene inheritance, segregation and expression in bread wheat. Euphytica 129: 301-309   DOI   ScienceOn
12 Lonsdale DM, Moisan LJ, Harvey AJ (1998) The effect of altered codon usage on luciferase activity in tobacco, maize and wheat. Plant Cell Pep 17: 396-399   DOI   ScienceOn
13 Lonsdalle D, Ontec S, Cuming A (1990) Transient expression of exogenous DNA in intact, viable wheat embryos following particle bombardment. J Exp Bot 41: 1161-1165   DOI
14 Chen WP. Gu X, Liang GH, Muthukrishnan S, Chen PD, Liu OJ, Gill BS (1998b) Introduction and constitutive expression of a rice chitinase gene in bread wheat using biolistic bombardment and the bar gene as a selectable marker. Theor Appl Genet 97: 1296-1306   DOI   ScienceOn
15 Stoger E, Williams S, Dulliams, Keen D and Christou P (1998) Molecular characteristics of transgenic wheat and the effect on transgene expression. Transgenic Res 7: 463-471   DOI
16 Felfoldi K, Purnhauser L (1992) Introduction of regenerating callus cultures from immature embryos of 44 wheat and 3 triticale cultivars. Cereal Research Communications 20: 273-277
17 Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylaor ML, Rodriguez D, Pajeau ME, Brown SM, Santino CG, Fry JE (1995) Glyphosate-tolerant CP4 and Gox genes as a selectable marker in wheat transformation. Plant Cell Rep 15: 159-163   DOI
18 Altpeter F, Vasil, V, Srivastava V, Stoger E, Vasil IK (1996) Accelerated production of transgenic wheat (Triticum aestivum L) Plants. Plant Cell Rep 16: 12-17   DOI   ScienceOn
19 Zhao H, Li M-Y, Pei Y, Dong Y-M (2002) Studies on the transfer of herbicide-resistance gene into cultivated wheat by biolistics. Chinese Bulletin of Botany 19: 457-461
20 Blechl A, Anderson D (1996) Expression of a high-molecularglutenin subunit gene in transgenic wheat. Nature Biol Technol 14: 875-879   DOI   ScienceOn
21 Jefferson RA (1987) Assaying chimeric genes in plants: The GUS fusion system. Plant Mol Biol Rep 5: 387-405   DOI
22 Weeks JT, Anderson aD and Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum L.). Plant Physiol 102:1077-1084   DOI
23 Vasil V, Castillo AM , Fromm ME, and Vasil V (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/Technology 10: 667-674   DOI
24 Vasil V, Srivastava V, Castillo AM, Fromm ME and Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Biol Technology 11: 1553-1558   DOI   ScienceOn
25 Barro F, Rooke L, Bekes F, Gras P, Tatham AS; Fido R, Lazzeri PA, Shewry PR, Barcelo P (1997) Transformation of wheat with high molecular weight subunit genes results in improved functional properties. Nature Bio/Technol 15: 1295-1299   DOI   ScienceOn
26 Becker D, Brettschneider R, Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J 2: 299-307
27 Fennell S, Bohorova N, Ginkel M, Crossa J, Hoisington D (1996) Plant regeneration from immature embryos of 48 elite CIM-MYT bread wheat. Theor Appl Genet 92: 163-169   DOI   ScienceOn
28 Zhang L, Rybczynski J, Langerberg W, Mitra A, French R (2000) An efficient wheat transformation procedure: Transformed calli with long-term morphogenic potential for plant regeneration. Plant Cell Rep 19: 241-250   DOI   ScienceOn
29 Klein TM, Wolf ED, Wu R, Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70-73   DOI
30 Liu W-H, Li W-X, Hu S-L, Xu X-L, Li J-L (2002) Study on influencing factors of tissue culture and biolistic bombardment in wheat. Acta Bot Boreal Occident Sin 22: 602-610
31 Barro F, Cannell ME, Lazzeri PA, Barcelo P (1998) The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants. Theor Appl Genet 97: 648-695
32 Alison H, Lisa M, Suzanne L, David L (1999) Wheat regenerated from scutellum callus as a source of material for transformation. Plant Cell Tiss Org Cult 57: 153-156   DOI   ScienceOn
33 Gabriela MP, Wilkinson MD, Steele SH, Sparks CA, Jones HD. and Parry MAJ (2001) Age-dependent transformation frequency in elite wheat varieties. J Exp Bot 52 357:857-863