• Title/Summary/Keyword: breeding control

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Studies on the Biological Control of Pine Caterpillar (Dendrolimus spectabilis Butler) by Red Wood Ants (Formica rufa truncicola var. yessoensis Forel) (불개미를 이용한 송총의 생물적방제에 관한 연구)

  • Kim Chang Hyo;Choi Jin Sik
    • Korean journal of applied entomology
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    • v.15 no.1 s.26
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    • pp.7-16
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    • 1976
  • In order to increase utility efficiency of red wood ants, Formica rufa truncicola var. yessonesis Forel as a resource of natural enemy of pine caterpillar, Dendrolimus spectabilis Butler, by finding out ecological and environmental factors in the habitat of red wood ants, the nest distribution and its density in habitat, plant distribution and density, stand-density of red pine, nest building and fixing plants, relative humidity of surface soil, physical and chemical natures of soil, and breeding rate were examined. The obtained results are summarized as follows: 1. The nest of red wood ants was densely distributed, in the lower-and middle top of mountain but no nest was found in the top. 2. The economical distribution of nest of habitat was estimated as $2.85/m^2$ and the lowest density as $1.93/m^2$ and these estimation lead us to confirm that pine caterpillar could be controlled. 3. The ecological characteristics of habitat seemed to be represented as higher stand-density of red pine of 10-20 years of age with large areas of eroded land under trees. The major grasses prevailing in this area were Andropogon brevifolius. Arundinella hirta, Miscanthus purpurasens, Eulia speciosa, Themeda japonica, Cymbopogon goeringii, and Eccoilpus cotulifer 4. Red wood ants seemed to build the nest by using red pine, Arundinella hirta, Miscanthus purpurascens, Themeda japonica or Cymbopogon goeringii as a fixing plant. 5. The limited point of humidity percent in habitat of red wood ants was estimated as $76\%$ during the acting period of May to September and as $72\%$ during pre-period of hibernation of October to November. 6. Soil analysis in habitating region showed higher concentration of organic matters and lower concentration of calcium and magnesium, and habitat was largely composed of silt and fine sand rather than coarse sand. 7. When the separated colony was transplanted to non-habitating red pine forest that seemed to have the similiar conditions as those of habitat, propagation and establishment of nest was possible.

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Association of Melanocortin 4 Receptor (MC4R) Genotypes with Fatty Acid Compositions in an Intercross Population between Duroc and Jeju Native Pigs (제주재래돼지와 듀록 참조축군에서 Melanocortin 4 Receptor (MC4R) 유전자형과 지방산 조성간의 관련성 분석)

  • Kang, Yong-Jun;Kim, Sang-Geum;Kim, Su-Yeon;Shin, Moon-Cheol;Woo, Jae-Hoon;Kim, Nam-Young;Shin, Sang-Min;Choi, Jae-Young;Yoo, Ji-Hyun;Park, Nam-Geon;Yang, Byoung-Chul;Cho, In-Cheol
    • Journal of Life Science
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    • v.30 no.1
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    • pp.58-63
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    • 2020
  • This study was conducted to examine association between melanocortin-4-receptor (MC4R) genotypes and fatty acid (FA) composition in an F2 intercross between Duroc and Jeju (South Korea) Native pigs (JNP). Fourteen FA composition traits were measured in more than 290 F2 progeny population produced between Duroc and JNP. All experimental pigs were successfully genotyped for the MC4R c.1426A>G (p.Asp298Asn) single nucleotide polymorphism (SNP) by using Taq I PCR-RFLP methods. We detected three MC4R genotypes, AA, AG, and GG with 0.299, 0.542, and 0.159 genotype frequencies, respectively. The MC4R AA genotype animals showed higher levels in palmitic acid (C16:0, p<0.05), stearic acid (C18:0, p<0.01), eicosenoic acid (C20:1n9, p<0.05), saturated fatty acid (SFA, p<0.01) than GG homozygotes, respectively. Whereas MC4R GG genotype showed higher values in linoleic acid (C18:2n6, p<0.001), linolenic acid (C18:3n3, p<0.001), linolenic acid (C18:3n6, p<0.001), arachidonic acid (C20:4n6, p<0.001) and unsaturated fatty acid (USFA, p<0.01) than AA pigs, respectively. The MC4R GG genotype was associated with increasing USFA and decreasing SFA in the F2 progeny population produced between Duroc and JNP. Our finding suggests that the MC4R polymorphisms can be used as a valuable genetic marker for Duroc and JNP breeding programs to improve meat quality and to control FA compositions.

Studies on the Heteroplastic Grafting of Carpathian Walnut (Carpathian 호도(胡桃)나무의 종간품종간접목(種間品種間接木)에 관(關)한 연구(硏究))

  • Park, Kyo Soo
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.95-108
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    • 1984
  • In order to see the effect of various factors on the success of grafting of walnut, a modified cleft grafting experiment has been conducted with Juglans sinensis Dode, J. regia (persian walnut), J. regiu (carpathian walnut), J. mandshurica, J. nigra and five cultivars of Carpathian walnut as "HANSEN", "METCALFE" "SCHOOL", "ILL CRATH", "LAKE" in a large green house equipped with automatic environmental control system in which temperature of the grafting bed at $28^{\circ}C$ and 85-100% for humidity during the period of January 1981-June 1983. As results of the experiments, the following facts were observed. 1) Hteroplastic grafting was proved to be more successful than homoplastic grafting with all five species tested among the J. regia (carpathian walnut), J. regia (persian walnut), J. sinensis, J. mondshurica, J. nigra. 2) It was interesting to notice that results hight survival of graft high as 99.33-100% with heteroplastic grafting when "Persian Walnut" and "Carpathian Walnut" was grafted on the stock of J. mandshurica and J. nigra. 3) A statistical significance of 5% Level was recognized in the above stocks among five species of homoplastic grafting with heteroplastic grafting and no significance among the scions. 4) The heteroplastic grafting when five cultivars of Carpathian Walnut as above as was grafted on the stock of Juglans nigra and J. mandshurica resulting high survival of graft as high as 85.33-100%. 5) As conclusion, the heteroplastic grafts of Carpathian Walnut and Persian Walnut was grafted on the stock of Juglans nigra and Jugdans mandshurica, the graft union, between stock and scion completed, in short period and was followed by a vigorous growth as well as "Cross Breeding", or hybrization, became apparent, with different desirable traits which could he used to cultivate a Later generation that combined these characteristics.

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Effect of N-Methylacetamide Concentration on the Fertility and Hatchability of Cryopreserved Ogye Rooster Semen (N-Methylacetamide 동결 보호제의 농도가 오계 동결 정액의 수정 및 부화율에 미치는 영향)

  • Kim, Sung Woo;Choi, Jin Seok;Ko, Yeoung-Gyu;Do, Yoon-Jung;Byun, Mijeong;Park, Soo-Bong;Seong, Hwan-Hoo;Kim, Chong-Dae
    • Korean Journal of Poultry Science
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    • v.41 no.1
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    • pp.21-27
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    • 2014
  • To preserve chicken genetic materials like cryopreserved spermatozoa, various kinds of freezing agents like glycerol, dimethylsuloxide, dimethylformamide or dimethylacetamide have been used for rooster semen preparation. Recently, the usage of N-methylacetamide (MA) for Ogye rooster semen preservation resulted in hatched chicken successfully. In this study, we investigated the effects of 7, 9 and 11% of MA on the viability, fertility and hatchability of frozen-thawed rooster semen using artificial insemination. The results of viability, fertility and hatchability in frozen semen with 7%, 9% or 11% MA were $35.16{\pm}6.12%$, $67.83{\pm}15.3%$ and $66.2{\pm}16.3%$ of motile sperm rate, 21.5%, 34.7% and 25% of fertility rate, and 100%, 89.5% and 87.5% of hatchability rate. The results of control group with frozen semen were 96.0% of fertility rate and 92.2% of hatchability rate. With these results, the concentration range of MA as a freezing agent of rooster semen could be 7~9% of media. The higher concentration of 9 % MA could decrease the fertility rate of thawed semen not the rate of hatchability rate. So the use of MA without affecting fertility rate would be a key point of freezing method of rooster semen for poultry genetic resource preservation.

Collection of Adult and Larval Mosquitoes in U.S. Army Compounds in the Republic of Korea During 1979~1983 (한국에 주둔한 미군지역내에서의 모기 성충과 유충 채집(1979~1983))

  • ;R.K
    • Parasites, Hosts and Diseases
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    • v.22 no.1
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    • pp.102-108
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    • 1984
  • This paper is the result of adult female mosquito and larval collection in U.S. Army Installations in Korea from 1979 to 1983. New Jersey light traps were operated for adult collection from May to October. The primary concern of this surveillance is to determine when to recommend insecticide spraying for mosquito control in the Army areas. The 5th Preventive Medicine Unit have developed an "index" level of female mosquitoes in a light trap similar to other U.S. Army Agencies in other parts of the world. When 10 female mosquitoes are reached on two consecutive trap-night, or 5 known vector females are collected, fogging is recommended in the trap areas. 1. Mosquito collections were conducted in 12 U.S. Army areas by operating 39 New Jersey light traps. Mosquitoes collected from the areas were identified to be 17 species comprising 3 genera. Anopheles sinensis (40%), Culex tritaeniorhynchus (31%), Aedes vexans nipponii (19 %) and Culex pipiens pallens (10%) appeared to be the most common species in the areas. 2. The species, population density and monthly appearance of adult mosquitoes were found to be almost the same in the all provinces involved. And Japanese Encephalitis vector mosquitoes, Culex tritaeniorhynchus, showed their seasonal fluctuation from July to September with a peak in August each year. 3. Larval habitats confirmed in the Army areas were categorized into 16 types as shown in Table 3. The mosquito larvae collected in those habitats were identified to be 15 species representing 4 genera. Most breeding sites in the Army areas were those which are activated during the wet season. 4. More mosquitoes were collected from the Kyungki Province than from the other Provinces. The reason for more collection of mosquitoes from military installations in the Kyungki Province appears to be the geographic characteristics surrounded by rice fields, marshes and other stagnant water areas.

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Comparison of F1 and F2 hybrid on authorized silkworm variety (누에 장려품종간 교잡누에의 실용형질 비교)

  • Kim, Kee-Young;Kim, Mi-Ja;Ji, Sang-Duk;Kim, Nam-Suk;Kim, Yong-Soon;Sung, Gyoo-Byung;Park, Kwang-Young;Kang, Pil-Don
    • Journal of Sericultural and Entomological Science
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    • v.53 no.1
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    • pp.12-18
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    • 2015
  • This study was conducted to consider practical use of F2 hybrid and intervariety crossing for the improvement of silkworm eggs export and silkworm eggs manufacture. Now Kumokjam and Baegokjam come into wide use to sericulture farmers but comparison test of F1, F2 hybrid and intervariety have not been done. We weighed against the productivity among economical character. While larval period of F2 among them is most long time, pupation percentage and cocoon yield per 10,000 3rd molted larvae are lower than intervariety. With comparing F2 and intervariety, single cocoon weight of F2 is lower 9 ~ 21% than intervariety, and cocoon shell weight of F2 was lower 10 ~ 18% than intervariety. By number of cocoons per liter, F2 hybrid was lower and more irregular size than intervariety. In coparison with number of egg laid, F2 hybrid Baegokjam was 655 ea (14%) and Kumokjam 625 ea (40%) more than F1 hybrid. Also, F2 hybrid for filament grade went down than F1 hybrid. The advantage of egg production in F2 hybrid can reduce costs because there is no necessity for sex discrimination and germination control. But besides increase of number of egg laid, the productivity including economical characters was greatly lowered. Except for being a lot of laying eggs, cocoon productivity of the F2 was greatly reduced by degeneration of practical traits. Therefore the further studies are needed in order to increase utilization value of the F2 hybrid silkworm.

Analysis of Quantitative Trait Loci (QTLs) for Seed Size and Fatty Acid Composition Using Recombinant Inbred Lines in Soybean (콩 재조합자식계통을 이용한 콩 종자의 크기와 지방산 조성의 양적 형질 유전자좌 분석)

  • Kim, Hyeun-Kyeung;Kim, Yong-Chul;Kim, Sun-Tae;Son, Beung-Gu;Choi, Yong-Whan;Kang, Jum-Soon;Park, Young-Hoon;Cho, Young-Son;Choi, In-Soo
    • Journal of Life Science
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    • v.20 no.8
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    • pp.1186-1192
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    • 2010
  • Soybean [Glycine max(L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvements in economic traits, such as quality and oil constituents, arethe most important objectives in soybean breeding. The objective of this study was to identify quantitative trait loci (QTLs) that control seed size and fatty acid contents in soybean. 115 $F_{2:10}$ recombinant inbred lines (RIL) developed from a cross of 'Keunolkong' and 'Iksan10' were used. Narrow-sense heritability estimates based on a plot mean on 100 seed weight, saturated fatty acid (palmitic acid + stearic acid), and oleic, linoleic, and linolenic acid content were 0.72, 0.60, 0.83, 0.77 and 0.81, respectively. The 100 seeds weight was related to seven QTLs located on chromosomes 1, 3, 8, 9, 16 and 17. Two independent QTLs for saturated fatty acid content were identified on chromosomes 17 and 19. Five independent QTLs for oleic acid content wereidentified on chromosomes7, 11, 14, 16 and 19. Five QTLs for linoleic acid content were located on chromosomes 2, 11, 14, 16 and 19. Three QTLs for linolenic acid content were located on chromosomes 8, 10 and 19. Oleic, linoleic, and linolenic acid had one major common QTL on chromosome 19. Thus, linoleic and linolenic acid content were identified as common QTLs.

Differences of Wood Vinegar Ingestion and Exercise Training on Blood Lipids, MDA, and SOD Activities in Rats (4주간 흰쥐의 목초액 섭취와 운동에 따른 혈중지질과 MDA, SOD 활성 차이)

  • Kang, Jong-Suk;Kim, Sea-Hyun;Kim, Pan-Gi;Lee, Dong-Woon;Ryu, Sung-Pil
    • Journal of Life Science
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    • v.19 no.9
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    • pp.1190-1199
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    • 2009
  • The purpose of this study is to identify the effects of exercise training and oak tree wood vinegar ingestion on the blood lipids and antioxidant activities of rats. The subjects were 28 Sprague Dawley male rats, and they were assigned into four groups (n=7, respectively): the control group (CON), the exercise group (EXE), the vinegar ingestion group (VIN), and the vinegar ingestion and exercise training group (VINEXE). The diet was based on high fat and oral administration of oak tree wood vinegar. The rats that were not given oak tree wood vinegar were given the same amount of distilled water orally in order to maintain the same level of stress. They were exercise trained on motor-driven treadmills during a four-week session. Weight changes in the VINEXE were significantly inhibited in the later period of exercise, when compared to the CON (p<0.05). Fat increase was significantly suppressed in VIN and EXE (p<0.05), and a synergistic effect was discovered in the VINEXE (p<0.05). Glucose and ammonia levels were significantly reduced in the EXE, VIN, and VINEXE compared to the CON (p<0.05). In blood lipids, TC and LDL-C were significantly enhanced in the EXE, VIN, and VINEXE compared to the CON (p<0.05), while HDL-C was significantly improved in the EXE and VINEXE (p<0.05). Liver MDA contents showed significant changes in each group (p<0.05), and SOD activities were significantly enhanced in the VIN and the VINEXE when compared to other groups (p<0.05). Therefore, oak tree wood vinegar ingestion with exercise training for four weeks may result in inhibition of weight gain, improvement of blood lipids, and inhibition of lipid peroxidation, contributing to health promotion.

Variation Patterns of the Blood Lipid Levels on the Sprague-Dawley-Rats Fed with Kochujang Extracts (고추장 추출물을 급여한 Sprague-Dawley-Rat에서의 혈중지질 농도의 변화 양상)

  • Woo, Yong-Ku;Kim, Tae-Hyo;Koh, Jong-Ho
    • The Korean Journal of Food And Nutrition
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    • v.26 no.4
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    • pp.737-744
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    • 2013
  • The present study was investigated to evaluate on the health improving effect of Kochujang (Fermented Korean hot pepper paste), which was famous as Korean traditional healthy food among foreigners as it was served together with Bibimbap. For comparison, observation was made on the experimental animals (Sprague-Dawley rat) as breeding during 5 weeks, which were divided three groups as into the control groups (N-group) fed with normal feed only and high cholesterol feed only (H-group) and the K-group with combined diet of normal feed and Kochujang extracts (Water soluble complex materials). The most outstanding result was found as the definite fatty liver lesions found from all (100%) of the rats of the H-group at the abdominal opening examination. On the other hand, there was any finding on fatty liver lesions in the N-group as well as the K-group. Therefore, it was more than enough to identify the hypolipidemic effect by visual finding only. Furthermore, from the results of blood plasma analysis from SD-rats fed with Kochujang extract (K-group), more prominent differential findings were found, the value of HDL-cholesterol, which was good for preventing the arteriosclerosis, was more or less higher than that of H-groups. But the level of LDL-cholesterol, which was one of the hazardous agents of hyperlipidemia and arteriosclerosis, was more significantly reduced to the level even lower than that of N-group, based on the statistical analysis, in especial. According to the present results, we could find that the unidentified Korean traditional Kochujang-derived complex components had expressed both the positive health improving effects and hypolipidemic effects on the SD-rat.

Characteristics and breeding of a new cultivar Pleurotus eryngii, Song-A (큰느타리버섯 신품종 '송아'의 육성 및 그 특성)

  • Shin, Pyung-Gyun;Park, Yun-Jung;Yoo, Young-Bok;Kong, Won-Sik;Jang, Kab-Yeul;Cheong, Jong-Cheon;Oh, Se-Jong;Lee, Keum-Hee
    • Journal of Mushroom
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    • v.9 no.2
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    • pp.59-62
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    • 2011
  • To develop a new cultivar of King oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of Di-mon crossing between monokaryotic strains derived from ASI 2824(Keunneutari No.2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-51($G09-21-10{\times}2844-11$) was shown the best cultural characteristics, selected to be a new cultivar and named as 'Song-A'. The 'Song-A' was formed incompatibility line distinctly in the confrontation growth of parental strains Keunneutari No.2, Aeryni 3 and ASI 2844. The optimum temperature for mycelial growth, fruiting body development and pH arrange were $25{\sim}30^{\circ}C$, $14{\sim}16^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about $94.7{\pm}29.5$ g which is almost 106% quantity compared to that of other cultuvar Keunneutari No.2. And also the stip is thick and long but the number of available stipe is few. Analysis of the genetic characteristics of the new cultivar 'Song-A' showed a different DNA profile as that of the control strains, Keunneutari No.2, Aeryni 3 and ASI 2844, when RAPD(Random Amplified Polymorphic DNA) primers URP4 and 7 were used. This new cultivar 'Song-A' of Pleurotus eryngii is characterized by a small number of primordia formation and the stip is thick and long. Therefore, we expect that this new strain will save of labor and cost by without culling work.