• 한국동물위생학회지
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• 제21권1호
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• pp.1-12
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• 1998

As the result of epidemiological analysis on bovine tuberculosis in Kyonggi province during the last ten years(1987-1996), 1. The annual incidence number of bovine tuberculosis positive farms in Kyonggi province fell down from 81 in 1987 to 14 in 1989. But the incidence is increased since 1990, in 1995 the incidence number was 102 farms of the most incidence. Total number of bovine tuberculosis positive farms were 456 farms(56.7%) in Kyonggi province during the 1987-1996 period. 2. The developmental trends of bovine tuberculosis positive heads was similiar to that of positive farms. Since 1993, the incidence was suddenly increased. Total number of bovine tuberculosis positive heads were 1,015 head(64.3%) in Kyonggi province during the 10 years. 3. Average incidence for rate during the last ten years(1987-1996) was 0.10%. The positive rate was suddenly increased since 1993 and in 1996, the positive rate was 0.23% of the highest incidence. 4. Regional Incidence number of bovine tuberculosis positive heads in Kyonggi province during the last seven years(1990-1996) was the most in Hwasung, Pochon, Pyungtek in order of Incidence and bovine tuberculosis positive density was 1.297% of the highest in Buchon. 5. In 244 farms(82.4%), bovine tuberculosis first occurred during the last seven years (1990-1996) but in farms over second occurrence, the number of bovine tuberculosis positive heads were 520 heads(58.2%).

• 한국동물위생학회지
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• 제30권4호
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• pp.527-532
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• 2007

In present study, we described the reliability of the dual priming oligonucleotide (DPO) multiplex polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis complex (MTC) and non-Mycobacterium tuberculosis (NMT) in blood samples of the Korea native cattle, Hanwoo. Among 340 samples 22 (6.5%) were positive in using DPO multiplex PCR, 21 (6.2%) were positive in PCR. The relative agreement between 2 tests was 99.7%, and the agreement quotient (kappa), was 0.95 (excellent). In these results, we demonstrated the successful application of DPO multiplex PCR for the diagnosis of bovine tuberculosis in Hanwoo. Multiplex PCR, using DPO primers, can be useful for the simple diagnosis of bovine tuberculosis in bovine blood samples.

• 한국동물위생학회지
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• 제41권2호
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• pp.71-78
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• 2018

Bovine tuberculosis (bTB) is a wide-spread zoonotic disease in cattle, which is casued by Mycobacterium bovis that is a part of Mycobacterium tuberculosis complex (MTC). This study describes a field trial conducted in 42 herds with the history of prevalence bovine tuberculosis. Two cell-mediated immunity tests, the gamma-interferon (${\gamma}-IFN$) assay and the single intradermal tuberculin test (SIT) were applied for the diagnosis of bovine tuberculosis in 5,289 animals. The ${\gamma}-IFN$ assay presented 144 (2.7%) head of cattle with the positive result, and 112 (2.1%) head of cattle were shown to be bTB-positive by the SIT. The positive concordance was 45.5%, and the negative concordance was 98.2%. The ${\gamma}-IFN$ assay showed more positive results in younger cattle, especially between 12 and 23 months of age. It is shown that the strategic combination of both cell-mediated immunity test methods is more efficient for the detection of bTB to reduce the number of false positive individuals which are being slaughtered.

• 한국동물위생학회지
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• 제36권2호
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• pp.79-86
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• 2013

Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10 $fg/{\mu}l$. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.

• 대한수의학회지
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• 제60권2호
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• pp.85-88
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• 2020

A 12-month-old domestic rabbit died suddenly without specific clinical signs. Gross examination revealed irregular yellowish hepatic nodules with pus in the submandibular muscles, lungs, uterus, and small intestines. Histopathological examination of the liver showed granulomatous inflammation with acid-fast-positive bacteria. Mycobacterium bovis SB1040 was identified by polymerase chain reaction and spoligotyping, and Pasteurella multocida was isolated from the multiple lesions. This report demonstrates the pathological features of rare bovine tuberculosis (bTB) in a domestic rabbit, the first case in the Republic of Korea. To ensure public safety, we recommend routine monitoring of rabbits to control the incidence of bTB.

• Journal of Preventive Veterinary Medicine
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• 제41권2호
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• pp.79-83
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• 2017

In this study, we used a choropleth map to explore the spatial variation of the risk of cattle herds being bovine tuberculosis (BTB) positive in Gangwon-do in 2015. The map shows that the risk of being BTB-positive was lower in provinces located in the middle of Gangwon-do (Wonju, Youngwol, Peongchang, and Kangneung) than in other provinces. In addition, one province located in the north (Goseong) had a low risk of BTB. The estimate for the intercept of the spatial lag model was 0.66, and the spatial autocorrelation coefficient (lambda) was 0.20 (Table 1). The Moran's I was 0.33 with p-value of 0.02. In 2015, provinces located in the North West (Hwacheon) and East (Donghae) of Gangwon-do had a higher BTB risk. We identified some specific provinces at low BTB-positive risk, information that may prove useful for control of BTB in the study area.

• 한국동물위생학회지
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• 제33권4호
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• pp.335-340
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• 2010

On the basis of the 2009 business plan, 20,394 Korean native cattle and beef cattle were carried examination of bovine tuberculosis by using ELISA technique from March to December. As a result, 66 cattle tested positive for tuberculosis and showed 0.32% positive ratio. Intradermal tuberculin test about 66 cases of ELISA positive cattle was carried out, and all of 66 cattle were confirmed as negative. However, when 7 PPD-positive cattle derived from slaughterhouse were tested by 20k ELISA kit and MS ELISA kit, 3 (2 suspect) cattle and 5 cattle showed positive results, respectively. As compared to the results of PPD test, the concordance rates were 43% (71% included suspect) with 20k ELISA kit and 71% with MS ELISA kit.

• 한국수의병리학회지
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• 제6권2호
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• pp.53-60
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• 2002

• 한국동물위생학회지
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• 제34권4호
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• pp.321-331
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• 2011

Mycobacterium bovis, a member of the M. tuberculosis complex (MTC), is the causative agent of bovine tuberculosis. Detection of M. bovis and M. tuberculosis using conventional culture- and biochemical-based assays is time-consuming. Therefore, a simple and sensitive molecular assay for rapid detection would be of great help in specific situations such as faster diagnosis of bovine tuberculosis (bTB) infection in the abattoirs. We developed a novel multiplex real-time PCR assay which was applied directly to biological samples with evidence of bTB and it was allowed to differentiate between M. bovis and M. tuberculosis. The primers and TaqMan probes were designed to target the IS1081 gene, the multi-copy insertion element in the MTC and the 12.7-kb fragment which presents in M. tuberculosis, not in the M. bovis genome. The assay was optimized and validated by testing 10 species of mycobacteria including M. bovis and M. tuberculosis, and 10 other bacterial species such as Escherichia coli, and cattle lymph nodes (n=113). The tests identified 96.4% (27/28) as M. bovis from the MTC-positive bTB samples using conventional PCR for specific insertion elements IS1081. And MTC-negative bTB samples (n=85) were tested using conventional PCR and the real-time PCR. When comparative analyses were conducted on all bovine samples, using conventional PCR as the gold standard, the relative accuracy of real-time PCR was 99.1%, the relative specificity was 100%, and the agreement quotient (kappa) was 0.976. The detection limits of the real-time PCR assays for M. bovis and M. tuberculosis genomic DNA were 10 fg and 0.1 pg per PCR reaction, respectively. Consequently, this multiplex real-time PCR assay is a useful diagnotic tool for the identification of MTC and differentiation of M. bovis and M. tuberculosis, as well as the epidemiologic surveillance of animals slaughtered in abattoir.

• 한국동물위생학회지
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• 제30권3호
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• pp.407-414
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• 2007

There were eight Korean indigenous cattles affected with bovine tuberculosis (BTB) detected by inspectors at slaughterhouses located in Chungbuk province from May 2006 through July 2007. Postmortem finding of BTB cases was characterized by the presence of several caseous or calcified nodules encapsulated by connective tissue from the pleural/peritoneal surface, livers, lungs and regional lymph nodes On micro-scopic examinations, the characteristic lesion of BTB was the formation granulomatous nodules, which contains central calcified necrotic zone surrounded by epithelioid cells, macrophages and a few Langhans' type giant cells. In addition, mononuclear cells and fibroblasts were also infiltrated. At the periphery, encapsulation was formed that protect the neighboring healthy tissues.