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http://dx.doi.org/10.7853/kjvs.2018.41.2.71

A comparative study of the gamma-interferon assay and the single intradermal tuberculin test for the diagnosis of bovine tuberculosis under field conditions  

Ha, Min-Jong (East-Branch, Gyeongbuk Veterinary Service Laboratory)
Oh, Gyeong-Min (East-Branch, Gyeongbuk Veterinary Service Laboratory)
Kim, Sang-Yun (East-Branch, Gyeongbuk Veterinary Service Laboratory)
Do, Jae-Cheul (West-Branch, Gyeongbuk Veterinary Service Laboratory)
Lee, Young-Ju (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Service / v.41, no.2, 2018 , pp. 71-78 More about this Journal
Abstract
Bovine tuberculosis (bTB) is a wide-spread zoonotic disease in cattle, which is casued by Mycobacterium bovis that is a part of Mycobacterium tuberculosis complex (MTC). This study describes a field trial conducted in 42 herds with the history of prevalence bovine tuberculosis. Two cell-mediated immunity tests, the gamma-interferon (${\gamma}-IFN$) assay and the single intradermal tuberculin test (SIT) were applied for the diagnosis of bovine tuberculosis in 5,289 animals. The ${\gamma}-IFN$ assay presented 144 (2.7%) head of cattle with the positive result, and 112 (2.1%) head of cattle were shown to be bTB-positive by the SIT. The positive concordance was 45.5%, and the negative concordance was 98.2%. The ${\gamma}-IFN$ assay showed more positive results in younger cattle, especially between 12 and 23 months of age. It is shown that the strategic combination of both cell-mediated immunity test methods is more efficient for the detection of bTB to reduce the number of false positive individuals which are being slaughtered.
Keywords
Bovine tuberculosis; ${\gamma}-IFN$ assay; Single intradermal tuberculin test; Diagnosis; Cell-mediated immunity;
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