Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Loop-mediated isothermal amplification assay for differentiation of Mycobacterium bovis and M. tuberculosis

Mycobacterium bovis와 M. tuberculosis 감별을 위한 등온증폭법

  • Koh, Ba-Ra-Da (Health & Environment Research Institute of Gwangju) ;
  • Kim, Jae-Myung (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Sung, Chang-Min (Health & Environment Research Institute of Gwangju) ;
  • Ji, Tae-Kyung (Health & Environment Research Institute of Gwangju) ;
  • Na, Ho-Myung (Health & Environment Research Institute of Gwangju) ;
  • Park, Seong-Do (Health & Environment Research Institute of Gwangju) ;
  • Kim, Yong-Hwan (Health & Environment Research Institute of Gwangju) ;
  • Kim, Eun-Sun (Health & Environment Research Institute of Gwangju)
  • 고바라다 (광주광역시보건환경연구원) ;
  • 김재명 (농림축산검역본부 세균질병과) ;
  • 성창민 (광주광역시보건환경연구원) ;
  • 지태경 (광주광역시보건환경연구원) ;
  • 나호명 (광주광역시보건환경연구원) ;
  • 박성도 (광주광역시보건환경연구원) ;
  • 김용환 (광주광역시보건환경연구원) ;
  • 김은선 (광주광역시보건환경연구원)
  • Received : 2013.05.01
  • Accepted : 2013.06.12
  • Published : 2013.06.30
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Abstract

Mycobacterium (M.) bovis, a member of the M. tuberculosis complex (MTC), is a re-emerging, zoonotic agent of bovine tuberculosis whose prevalence probably depends on variations in direct exposure to cattle and ingestion of raw milk. Accurate species differentiation of M. bovis and M. tuberculosis is needed to distinguish between human and zoonotic tuberculosis. This study successfully developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection and differentiation of M. bovis and M. tuberculosis, however showed negative reactions in eight non-tuberculous mycobacteria (NTM) samples and ten other bacterial species. Sensitivity of this assay for detection of genomic M. bovis DNA was 10 $fg/{\mu}l$. And this assay successfully detected M. bovis in bovine clinical specimens. In conclusion, the LAMP assay is a simple and powerful tool for rapid detection of M. bovis in both pure bacterial culture and in clinical samples.

Keywords

References

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