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Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo  

Shin, Jong-Bong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
Park, Nam-Yong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
Kim, Yong-Hwan (Department of Veterinary Research, Gwangju City Institute of Health and Environment)
Cho, Ho-Seong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
Publication Information
Korean Journal of Veterinary Service / v.30, no.4, 2007 , pp. 527-532 More about this Journal
Abstract
In present study, we described the reliability of the dual priming oligonucleotide (DPO) multiplex polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis complex (MTC) and non-Mycobacterium tuberculosis (NMT) in blood samples of the Korea native cattle, Hanwoo. Among 340 samples 22 (6.5%) were positive in using DPO multiplex PCR, 21 (6.2%) were positive in PCR. The relative agreement between 2 tests was 99.7%, and the agreement quotient (kappa), was 0.95 (excellent). In these results, we demonstrated the successful application of DPO multiplex PCR for the diagnosis of bovine tuberculosis in Hanwoo. Multiplex PCR, using DPO primers, can be useful for the simple diagnosis of bovine tuberculosis in bovine blood samples.
Keywords
Bovine tuberculosis; Dual priming oligonucleotide; Multiple PCR;
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1 Biet F, Boschiroli ML, Thorel MF, et al. 2005. Zoonotic aspects of Mycobec terium bovis and Mycobacterium evium-intracellulare complex (MAC). Vet Res 36 (3): 411-436   DOI   ScienceOn
2 Pavlik I, Machackova M, Ayele WY, et al. 2002. Incidence of bovine tuberculosis in wild and domestic animals other than cattle in six Central European countries during 1990-1999. Vet Med Czech 47(5): 122-131
3 Fritsche A, Engel R, Buhl D, et al. 2004. Mycobacterium bovis tuberculosis: from animal to man and back. Int J Tuberc Lung Dis 8 (7): 903-904
4 Haas WH, Schilke K, Brand J, et at. 1997. Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa. Antimicrob Agents Chemother 41 (7): 1601-1603
5 Chimara E, Ferrazoli L, Leo SC. 2004. Mycobacterium tuberculosis complex differentiation using gyrB-restriction fragment length polymorphism analysis. Mem Inst Oswaldo Cruz 99 (7): 745-748   DOI   ScienceOn
6 Byun HS, Lee HJ, Lee SM, et al. 2007. Bovine tuberculosis found at slaughtered Korean indigenous cattles. Korean J Vet Serv 30(3): 407-414   과학기술학회마을
7 Niemann S, Richter E, Rsch-Gerdes S. 2000. Differentiation among members of the Mycobacterium tuberculosis complex by molecular and biochemical features: evidence for two pyrazinamide-susceptible subtypes of M. bovis. J Clin Microbiol 38 (1): 152-157
8 Chan CM, Yuen KY, Chan KS, et al. 1996. Single-tube nested PCR in the diagnosis of tuberculosis. J Clin Pathol 49 (4): 290- 294   DOI
9 Erwin PC, Bemis DA, McCombs SB, et al. 2004. Mycobacterium tuberculosis transmission from human to canine. Emerg Infect Dis 10(12): 2258-2310   DOI
10 Altman DG. 1999, Practical statistics for medical research. Chapman & Hall/ CRC Press, Boca Raton/London/ New York/Washington, DC
11 Kim YH, Al Haddawi MH, Cho HS, et al. 2001. Multiplex PCR for differential diagnosis of Mycobacterium species from bovine clinical samples. Korean J Vet Res 41 (4): 535-542
12 Grange JM. 2001. Mycobacterium bovis infection in human beings. Tuberculosis 81(1-2): 71-77   DOI   ScienceOn
13 Barouni AS, Augusto CJ, Lopes MT, et al. 2004. A pncA polymorphism to differentiate between Mycobacterium bovis and Mycobacterium tuberculosis. Mol Cell Probes 18 (3): 167 -170   DOI   ScienceOn
14 Chun JY, Kim KJ, Hwang IT, et al. 2007. Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene. Nucleic Acids Res 35 (6): e40   DOI   ScienceOn
15 Cosivi O, Grange JM, Daborn CJ, et al. 1998. Zoonotic tuberculosis due to Mycobacterium bovis in developing countries. Emerg Infect Dis 4(1): 59-70   DOI   ScienceOn
16 Sreevatsan S, Escalante P, Pan X, et al. 1996. Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. J Clin Microbiol 34 (8): 2007-2010