Background : Chlamydia pneumoniae is one of common causes in upper and lower respiratory infections. Isolating C. pneumoniae from clinical specimens is very difficult due to the characteristics of the organism. Recently, we succeeded in isolating C. pneumoniae from a Korean patient, who suffered from acute pharyngitis. This is the first isolate from a clinical specimen in Korea. Methods : We attained a nasopharyngeal swab from a 22-year-old female patient, and inoculated it on a monolayer of the Hep-2 cell line. After 8 passages, we found the inclusion bodies of C. pneumoniae by an immunofluorescence(IF) test. The species-specific monoclonal antibody IF staining and species-specific PCR were done to confirm the species of the isolate, and electron microscopy was used to characterize the morphology. Results : The isolated was confirmed to be C. pneumoniae by species-specific IF and PCR, and the strain was named LKK-1. The shape of the elementary body was round and with a narrow periplasmic space, as shown by electron microscopy, which is similar to the Japanese strain, but not the Western strain. Conclusion : We succeeded in isolating C. pneumoniae from a 22-year-old patient with acute pharyngitis, which is the first isolate in Korea. In the future, this Korean strain will be useful to the study of C. pneumoniae.
A traveltime tomography has been carried out by transforming electromagnetic data in frequency domain to wave-like domain. The transform uniquely relates a field satisfying a diffusion equation to an integral of the corresponding wavefield. But direct transform of frequency domain magnetic fields to wave-field domain is ill-posed problem because the kernel of the integral transform is highly damped. In this study, instead of solving such an unstable problem, it is assumed that wave-fields in transformed domain can be approximated by sum of ray series. And for further simplicity, reflection and refraction energy compared to that of direct wave is weak enough to be neglected. Then first arrival can be approximated by calculating the traveltime of direct wave only. But these assumptions are valid when the conductivity contrast between background medium and the target anomalous body is low enough. So this approach can only be applied to the models with low conductivity contrast. To verify the algorithm, traveltime calculated by this approach was compared to that of direct transform method and exact traveltime, calculated analytically, for homogeneous whole space. The error in first arrival picked by this study was less than that of direct transformation method, especially when the number of frequency samples is less than 10, or when the data are noisy. Layered earth model with varying conductivity contrasts and inclined dyke model have been successfully imaged by applying nonlinear traveltime tomography in 30 iterations within three CPU minutes on a IBM Pentium Pro 200 MHz.
Objectives : There have been several studies documenting the changing level of the conus throughout infancy and childhood, but there is only a little detailed study that documents the range of conus positions in a living adult population, especially in Korean, without spinal deformity. Methods : we made a sequential study of magnetic resonance images of the lumbar spine to determine the variation in position of the conus medullaris in 650 living korean adults population without spinal deformity who checked MRI to identify the cause of low back pain. The study population consisted of patients over the age of 16 years. A T1-weighted, midline, sagittal image was reviewed for identifying the postion of conus. This location was recorded in relation to the upper, middle, or lower third of the adjacent vertebral body or the adjacent intervertebral disc. Results : The study group consisted of 305 men(47%) and 345 women(53%) with a mean age 45.9 years(range, 16-79 years). The conus existed commonly at the middle third of L1(131cases, 20.2%), at the L1-2 intervertebral space(129cases, 19.8%), and the lower third of L1(123cases, 18.9%). The mean position of conus was the lower third of L1(range, middle third of T12 to middle third of L3). Conclusions : The mean position of conus was at the lower third of L1(range, middle third of T12 to middle third of L3). This results was same as that of foreign study. Our results of living korean adult population could allow for safe clinical procedures such as lumbar puncture, spinal anesthesia, and help to explain the differences among observed neurologic injuries from fracture-dislocation at the thoracolumbar junction.
KIPS Transactions on Computer and Communication Systems
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v.3
no.5
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pp.141-150
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2014
In recent years, because of the development of ubiquitous technology in healthcare research is actively progress. Especially, healthcare service area is change to home for the elderly or patients from hospital. The technology to identify residents in a home is crucial for smart home application services. However, existing researches for resident identification have several problems. In this case, residents are needed to attach of various sensors on their body. Also relating private life, it is difficult to apply to resident's environment. In this paper, we used constraint-free sensor and unconscious sensor to solve these problems and we limited using of sensor and indoor environment in the way of working economical price systems. The way of multi-resident identification using only these limited sensors, we selected elements of personal identifications and suggested the methods in giving the weight to apply these elements to systems. And we designed the SABA mechanism to tract their location and identify the residents. It mechanism can distinguish residents through the sensors located each space and can finally identify them by using the records of their behaviors occurred before. And we applied the mechanism designed for applications to approve this location tracking system. We verified to the location tracking system performance according to the scenario.
Park, Il-Dong;Ji, Kil-Ryong;Imm, Sung-bin;Kum, Ki-Jung
Journal of Korean Society of Transportation
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v.22
no.4
s.75
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pp.97-106
/
2004
It is a Known fact that driving through long tunnel increases possibility of traffic accident because of psychological feeling of insecurity and dispersion of drivers' concentration since driving in narrow and limited space for a longtime. It, therefore, results in raising transportation and environment problems, such as traffic accident difficult to be properly dealt with and ventilation. This study aims at proposing a method of augmenting driving amenity by improving the internal lighting facilities in the tunnel. The study is conducted by investigating internal landscapes of tunnels by lighting colors, which are currently being operated. The Color Planning System (CPS), developed by SHARP Co. Ltd, is exploited for selecting adjective that express the sensitivity image on lighting colors. The CPS is an example that applies to sensitivity of human body for products design development. The CPS takes the following process to define the color : 1) expressing "Pvoduct's Image" as "A Word (adjective)" and 2) referring "A Word" to "Image Scale", and 3) determining the color through this "Image Panel". The study is processed by making a questionnaire using the semantic differential (SD) scale, grasping the consciousness structure of experimental persons through the Factor Analysis, and building a model in which dependent variable is "Degree of Preference" about internal landscape in tunnel using LISREL(LInear Structural RELations).
To produce reconstituted rabbit embryos with fetal fibroblasts, the present study was evaluated the efficiencies of the different fusion and activation conditions as assessments of subsequent development and chromosome in the embryos. New Zealand White rabbits were used throughout the study. Fetal fibroblasts collected from 22-d of fetuses were cultured in DMEM + 10% FBS in 5% $CO_2$ in air. The culture was maintained for 10 passages. In every passage half of cell suspension were kept In frozen. From rabbits treated with FSH in 30% PVP solution and hCG, oocytes were surgically collected from oviducts at 14 h post-hCG injection and stripped off their cumulus cells by re-pipetting in a 300 IU hyaluronidase solution. Oocytes with an extruded first polar body and dense cytoplasm were enucleated by micromanipulation in Ham's F-10 medium+7.5 g/$m\ell$ cytochalasin B. Euncleation was confirmed under a fluorescence microscope after staining with 5 g/$m\ell$ bisbenzimide for 2 min. Each enucleated oocyte was injected with a fetal fibroblast into a perivitelline space. Reconstructed eggs were compared fusion rates either at 2.0 ㎸/cm or 1.6 ㎸/cm(60 sec, double pulses). After fusion, all eggs were activated with the combination of 5 M ionomycin (5 min) and 10 g/$m\ell$ cycloheximide (CHX, 3h), and cultured in CRlaa medium and transferred into TCM199+10% FBS on day 3. Although there was not significantly differ in fusion rate between treatments (60%, 2.0 ㎸/cm vs. 79.4%, 1.6 ㎸/cm), none of them in the eggs fused with 2.0 ㎸/cm developed to blastocyst. In comparison of development and chromosome status between different activation treatments (Group 1; 5 M ionomycin/10 g/$m\ell$ CHX, Group 2; 5 M ionomycin/5 g/$m\ell$ CHX + 2 mM DMAP after fusion with 1.6 ㎸/cm), there were not differ in cleavage and development rates (67.3% and 28.9% in Group 1; 67% and 33% in Group 2). All out of 8 embryos evaluated in Group 1 appeared a normal diploid chromosome sets and mean number of cells (Mean SEM) on day 4.5 of culture was 141.5 23.15 (n=8). It can be concluded that the use of cycloheximide has not happened in chromosome abnormalities, and fetal fibroblasts can be used for cloning in rabbit.
In order to know the effects of ginseng on the stress, histochemical and electron microsbopic observations were carried out of the adrenal cortex on the stressed rats. The experiments were carried out as follow : non-stressed after saline injection (S-NS-AD), stressed after saline injection (S-S-AD), non-stressed after ginseng injection (G-NS-AD), stressed after ginseng injection (G-S-AD). Saline and ginseng extract were treated by peritoneal injection for 30 days. The results obtained are summarized as follows : Histologically a number of small lipid droplets are found more in S-S-AD group than in S-NS-AD group, whereas in ginseng treated groups numerous large lipid droplets are found. Histochemically cholesterol and its ester, glycolipid, phospholipid and glycogen are highly found in ginseng-treated groups. On the other hand, glycogen and cholesterol are also observed in S-S-AD group. Electron microscopically a numbers of lipid droplets are observed in S-S-AD, G-NS-AD and G-S-AD group, especially large lipid droplets and moderate glycogen are found in G-NS-AD and G-S-AD group. In ginseng-treated groups and S-S-AD group, dilated SER, extended intercellular space and dense bodies are observed, and the filopodiae are found in G-S-AD group. These data suggest that ginseng was activate the metabolism and synthesis of the adrenal cortical cells on the stress.
Journal of the Korea Academia-Industrial cooperation Society
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v.19
no.7
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pp.283-304
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2018
mankind has evolved to stand upright a million years ago. However entering an industrial society, much has changed in only a few decades. Human beings spend more time sitting down and working, which negatively affects their bodies. Leading medical studies have shown that the prolonged working conditions have significantly affected the causes of adult illnesses that we have known. Human beings have evolved in a moving direction. So, like modern societies, sitting or standing in one space for long periods of time without movement brings various side effects to the body. Reflecting this background, this study has led to think of work in an environment that is taking the evolutionary biology perspective from a traditional standing, standing work environment. They also plan to conduct an experiment to handle work with smartphones in the hopes of expanding their mobile office environment to use smartphones in the field, which is a type of smart work. The study could help build and establish a mobile office environment, a type of smart work. In addition to using smartphones, a working environment can be created that works by moving around. Furthermore, the paradigm of the work environment of businesses and students could be changed.
Lee, Jong Un;Park, Ki Jeoung;Kim, Ki Hong;Choi, Man Kyu;Lee, Young Hwan;Kim, Dae-Hyun
Journal of Korean Neurosurgical Society
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v.63
no.5
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pp.614-622
/
2020
Objective : The method of approach during transforaminal endoscopic lumbar discectomy (TELD) has been the subject of repeated study. However, the ideal entry point during TELD has not been studied in detail. Therefore, this study investigated the ideal entry point for avoiding complications using computed tomography (CT) scans obtained from patients in the prone position. Methods : Using CT scans obtained from patients in the prone position, we checked for retroperitoneal or visceral violations and measured the angles of approach with five conventional approach lines drawn on axial CT scans at each disc space level (L2-3, L3-4, and L4-5). We also determined the ideal entry point distance and approach angles for avoiding retroperitoneal or visceral violations. Correlation analysis was performed to identify the patient characteristics related to the ideal entry point properties. Results : We found that the far lateral approach at the L2-3 level resulted in high rates of visceral violation. However, rates of visceral violation at the L3-4 and L4-5 levels were remarkably low or absent. The ideal angles of approach decreased moving caudally along the spine, and the ideal entry point distances increased moving caudally along the spine. Weight, body mass index (BMI), and the depth of the posterior vertebral line from the skin were positively associated with the distance of the ideal entry point from the midline. Conclusion : We reviewed the risk of the extreme lateral approach by analyzing rates of retroperitoneal and visceral violations during well-known methods of approach. We suggested an ideal entry point at each level of the lumbar spine and found a positive correlation between the distance of the entry point to the midline and patient characteristics such as BMI, weight, and the depth of the posterior vertebral line from the skin.
3-D ore modeling was performed to understand the configuration of ore bodies by integrated analysis of borehole and geophysical data in iron-mine area. Five representative indices of rocks were designated, which were obtained from geological survey and borehole. The five indices of rocks were geostatistically simulated by Sequential Indicator Simulation method to delineate boundary of the ore bodies. And Ordinary Kriging and Sequential Gaussian Simulation was applied to make secondary information using resistivity data from magnetotellurics and DC resistivity survey, and this information was used for simple kriging with local varying means, one of integrated kriging techniques. From the correlation analysis between each properties, it was found that high grade of ore is characterized by increased density, whereas the electrical resistivity decreases. With the integrated results of geophysical and borehole data, it was also found that the real configuration of ore body was similar to the modeled result and information about ore grade in 3-D space was obtained.
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