• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3793-3797
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• 2015

Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder of pluripotent stem cells, caused by reciprocal translocation between the long arms of chromosomes 9 and 22, t(9;22)(q34;q11), known as the Philadelphia chromosome. Materials and Methods: A total of 51 CML patients were recruited in this study. Complete blood counts of all CML patients were performed to find out their total leukocytes, hemoglobin and platelets. FISH was performed for the detection of BCR-ABL fusion and cryptogenic tests using bone marrow samples were performed for the conformation of Ph (9;22)(q34;q11) and variant translocation mechanisms. Results: In cytogenetic analysis we observed that out of 51 CML patients 40 (88.9%) were Ph positive and 4 (8.88%) had Ph negative chromosomes. Mean values of WBC 134.5 $10^3/{\mu}l$, hemoglobin 10.44 mg/dl, and platelets 288.6 $10^3/{\mu}l$ were observed in this study. Conclusions: In this study, Ph positive translocation between chromosome (9:22)(q34;q11) were observed in 40 (88.9%) CML patients.

• 공업화학
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• 제25권3호
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• pp.324-329
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• 2014

미세조류 Nannochloropsis oculata (N. oculata)와 Chlorella vulgaris (C. vulgaris)는 다른 미세조류에 비해 높은 중성지질 함유량으로 인해 바이오 디젤 생산에 중요한 자원이다. 나노기포 산소수, 수소수를 이용해 만든 배지에서 미세조류를 배양하였다. N. oculata 배양 결과 세포 성장은 대조군과 비슷하였으며 지질 함량은 대조군에 미치지 못하는 것으로 나타났다. C. vulgaris 역시 N. oculata와 비슷한 경향을 보였다. 미세조류의 광합성으로 인한 색소의 함량 변화를 알아보기 위해 클로로필과 카로티노이드 함량을 측정하였다. 클로로필 함량은 나노 기포산소수와 수소수에서 배양한 N. oculata가 대조군 보다 약 54%, 30% 증가하였으며 카로티노이드 함량은 각각 21%, 25%씩 증가함을 확인하였다. C. vulgaris의 클로로필 함량은 기포산소수와 수소수에서 대조군보다 59%, 39% 증가하였으며 카로티노이드 함량도 49%, 29% 증가하였다.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.224-229
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• 2009

Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.

• 한국환경성돌연변이발암원학회지
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• 제26권2호
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• pp.48-52
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• 2006

Our investigation of the seaweed extracts, Ulva lactuca. The biological activities antioxidant, antimicrobial activity, antifungal and haemolytic activity of ethy-ether and ethyl-acetate extracts from the seaweed, Ulva lactuca were investigated. They were separately extracted using ethyl-ether and ethyl-acetate from dried samples at room temperature and freeze dried. Seaweed extracts were found to cause significant free radical scavenging effects on DPPH (1,1-diphenyl-2-picrylhydrazyl). Seaweed extracts had not significant haemolytic activity against human erythrocyte. This extracts exhibited in vitro broad-spectrum antimicrobial activity of gram-negative, gram-positive bacteria and without antifungal activity.

• 한국생물공학회소식지
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• 제17권1호
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• pp.8-19
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• 2010

원문이미지 참조

• Journal of Microbiology and Biotechnology
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• 제23권11호
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• pp.1544-1553
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• 2013

Despite the importance of acetate kinase in the metabolism of bacteria, limited structural studies have been carried out on this enzyme. In this study, a three-dimensional structure of the Escherichia coli acetate kinase was constructed by use of molecular modeling methods. In the next stage, by considering the structure of the catalytic intermediate, trifluoroethanol (TFE) and trifluoroethyl butyrate were proposed as potential inhibitors of the enzyme. The putative binding mode of these compounds was studied with the use of a docking program, which revealed that they can fit well into the enzyme. To study the role of these potential enzyme inhibitors in the metabolic pathway of E. coli, their effects on the growth of this bacterium were studied. The results showed that growth was considerably reduced in the presence of these inhibitors. Changes in the profile of the metabolic products were studied by proton nuclear magnetic resonance spectroscopy. Remarkable changes were observed in the quantity of acetate, but other products were less altered. In this study, inhibition of growth by the two inhibitors as reflected by a change in the metabolism of E. coli suggests the potential use of these compounds (particularly TFE) as bacteriostatic agents.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.595-600
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• 1998

In order to investigate a relationship of the structure-antifungal and hemolytic activities between cecropin A(1-8)-magainin 2(1-12) and cecropin A(1-8)-melittin(1-12) hybrid peptides, several analogues with amino acid substitution at positions 10 (Ile) and 16 (Ser) were designed and synthesized. The increase of the hydrophobicity by substituting with Leu, Phe, and Trp at position 16 in cecropin A(1-8)-magainin 2(1-12) did not have a significant effect on antifungal activity but caused a remarkable increase in hemolytic activity. These results indicate that the hydrophobic property at position 16 of cecropin A(1-8)-magainin 2(1-12) is more correlated to hemolytic activity than to antifungal activity. Replacement with Pro at position 10 of cecropin A(1-8)- magainin 2(1-12) and cecropin A(1-8)-melittin (1-12) caused a remarkable decrease in a-helical contents in the 50% TFE solution and induced a reduction in lytic activity against Aspergillus flavus, and Aspergillus fumigatus. These results demonstrate that flexibility at the central hinge region is essential for lytic activity against fungal cells and $\alpha$-helicity of the peptides.

• 공업화학
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• 제23권6호
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• pp.594-598
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• 2012

본 연구에서는 해조류 다시마를 바이오매스로 이용하는 혐기성 소화로부터 바이오가스를 생산하였다. 최적의 혐기성 배양조건을 확립하기 위해 슬러지는 $-70^{\circ}C$에서 20 min 동안 냉동 전처리를 하였다. 대조군과 비교했을 때 수소와 메탄가스는 각각 2.7배와 3.4배 증가한 667.28 mL/L와 3420.24 mL/L를 생산하였다. 냉동 전처리한 슬러지의 초기 pH 최적 조건은 7.0이었고, 다시마 바이오매스의 초기 pH 최적 조건은 8.0이었으며 알칼리 조건에서 보다 산성 조건에서 바이오가스 생산량이 크게 감소하는 것을 확인할 수 있었다. 슬러지의 냉동 전처리와 바이오매스 및 슬러지의 최적 pH 조건에서 최대 643.73 mL/L의 수소와 4291.6 mL/L의 메탄가스를 생산하였다. 이는 대조군과 비교했을 때 각각 2.6배와 4.3배 증가한 생산량이었다. 또한 최적 조건의 5-L 회분식 혐기성 배양에서 바이오가스의 생산량을 측정한 결과 기질에 포함된 다시마에 의해 생산될 수 있는 최대 생산량은 수소 1605.03 mL/L와 메탄가스 4593.71 mL/L로 확인되었다.

• BMB Reports
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• 제50권9호
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• pp.478-483
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• 2017

Budding yeast has dozens of prions, which are mutually dependent on each other for the de novo prion formation. In addition to the interactions among prions, transmissions of prions are strictly dependent on two chaperone systems: the Hsp104 and the Hsp70/Hsp40 (J-protein) systems, both of which cooperatively remodel the prion aggregates to ensure the multiplication of prion entities. Since it has been postulated that prions and the remodeling factors constitute complex networks in cells, a quantitative approach to describe the interactions in live cells would be required. Here, the researchers applied dual-color fluorescence cross-correlation spectroscopy to investigate the molecular network of interaction in single live cells. The findings demonstrate that yeast prions and remodeling factors constitute a network through heterogeneous protein-protein interactions.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.386-387
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• 2011