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http://dx.doi.org/10.5010/JPB.2009.36.3.224

Development of transgenic sweet potato producing human lactoferrin  

Min, Sung-Ran (Plant Systems Engineering Research Center)
Kim, Jae-Wha (Medical Genomics Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Jeong, Won-Joong (Plant Systems Engineering Research Center)
Lee, Young-Bok (Dept. of Horticulture, Chungnam National University)
Liu, Jang R. (Plant Systems Engineering Research Center)
Publication Information
Journal of Plant Biotechnology / v.36, no.3, 2009 , pp. 224-229 More about this Journal
Abstract
Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.
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Times Cited By KSCI : 7  (Citation Analysis)
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