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http://dx.doi.org/10.5483/BMBRep.2017.50.9.084

Heterogeneous interaction network of yeast prions and remodeling factors detected in live cells  

Pack, Chan-Gi (Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine)
Inoue, Yuji (Department of Biomolecular Engineering, Graduate School of Biosciences and Biotechnology, Tokyo Institute of Technology)
Higurashi, Takashi (Department of Biochemistry, University of Wisconsin)
Kawai-Noma, Shigeko (Department of Biomolecular Engineering, Graduate School of Biosciences and Biotechnology, Tokyo Institute of Technology)
Hayashi, Daigo (Department of Biomolecular Engineering, Graduate School of Biosciences and Biotechnology, Tokyo Institute of Technology)
Craig, Elizabeth (Department of Biochemistry, University of Wisconsin)
Taguchi, Hideki (Department of Biomolecular Engineering, Graduate School of Biosciences and Biotechnology, Tokyo Institute of Technology)
Publication Information
BMB Reports / v.50, no.9, 2017 , pp. 478-483 More about this Journal
Abstract
Budding yeast has dozens of prions, which are mutually dependent on each other for the de novo prion formation. In addition to the interactions among prions, transmissions of prions are strictly dependent on two chaperone systems: the Hsp104 and the Hsp70/Hsp40 (J-protein) systems, both of which cooperatively remodel the prion aggregates to ensure the multiplication of prion entities. Since it has been postulated that prions and the remodeling factors constitute complex networks in cells, a quantitative approach to describe the interactions in live cells would be required. Here, the researchers applied dual-color fluorescence cross-correlation spectroscopy to investigate the molecular network of interaction in single live cells. The findings demonstrate that yeast prions and remodeling factors constitute a network through heterogeneous protein-protein interactions.
Keywords
Fluorescence cross-correlation spectroscopy; Live cell; Protein interaction; Remodeling factors; Yeast prions;
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